Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
An enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated syst...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2001-02-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200018 |
| _version_ | 1797712560864100352 |
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| author | Ma.Teresa Illnait Juan C Vilaseca Carlos M Fernández Gerardo F Martínez |
| author_facet | Ma.Teresa Illnait Juan C Vilaseca Carlos M Fernández Gerardo F Martínez |
| author_sort | Ma.Teresa Illnait |
| collection | DOAJ |
| description | An enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated system is highly sensitive and specific, and when it was compared with latex agglutination there were not significant differences. A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples. |
| first_indexed | 2024-03-12T07:23:35Z |
| format | Article |
| id | doaj.art-c2593c65cede44268d6e1ee491c35626 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:23:35Z |
| publishDate | 2001-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-c2593c65cede44268d6e1ee491c356262023-09-02T22:15:01ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602001-02-0196224124510.1590/S0074-02762001000200018Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigenMa.Teresa IllnaitJuan C VilasecaCarlos M FernándezGerardo F MartínezAn enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated system is highly sensitive and specific, and when it was compared with latex agglutination there were not significant differences. A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200018cryptococcal antigenantigen quantificationantigen detectionenzyme-linked immunosorbent assay-ELISA |
| spellingShingle | Ma.Teresa Illnait Juan C Vilaseca Carlos M Fernández Gerardo F Martínez Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen Memorias do Instituto Oswaldo Cruz cryptococcal antigen antigen quantification antigen detection enzyme-linked immunosorbent assay-ELISA |
| title | Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen |
| title_full | Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen |
| title_fullStr | Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen |
| title_full_unstemmed | Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen |
| title_short | Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen |
| title_sort | enzyme linked immunosorbent assay for detection and quantification of cryptococcus neoformans antigen |
| topic | cryptococcal antigen antigen quantification antigen detection enzyme-linked immunosorbent assay-ELISA |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200018 |
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