Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR)
Q fever is a widespread zoonotic disease that is caused by obligate intracellular bacteria, Coxiella burnetii. This study was planned to determine the prevalence of C. burnetii in cows’, goats’, and ewes’ bulk milk (BM) samples using PCR and to confirm positive results by DNA sequencing. A total of...
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| Format: | Article |
| Language: | English |
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Croatian Dairy Union
2015-02-01
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| Series: | Mljekarstvo |
| Online Access: | http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=197153 |
| _version_ | 1811329354395811840 |
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| author | Hayriye Yesim Can Mehmet Elmalı Alper Karagöz |
| author_facet | Hayriye Yesim Can Mehmet Elmalı Alper Karagöz |
| author_sort | Hayriye Yesim Can |
| collection | DOAJ |
| description | Q fever is a widespread zoonotic disease that is caused by obligate intracellular bacteria, Coxiella burnetii. This study was planned to determine the prevalence of C. burnetii in cows’, goats’, and ewes’ bulk milk (BM) samples using PCR and to confirm positive results by DNA sequencing. A total of 150 BM samples (50 samples of each cows’, goats’, and ewes’ milk) collected from 15 randomly selected dairy farms in Hatay province were analyzed. The BM samples were taken between January 2012 and July 2013. Bacterial DNA was extracted directly from milk samples. Nine of the BM samples (6 %) were PCR positive; five from cows’ BM, two from ewes’ BM, and two from goats’ BM. Bacterial DNA was detected in 3 of 15 (20 %) dairy farms. Positive results were confirmed by DNA sequencing. The sequencing results of Coxiella DNA extracted from cows’, goats’, and ewes’ milk samples were consistent with the reference strain with 100 %, 99.7 %, 99.8 % homology, respectively. BM samples were found to be contaminated with C. burnetii; therefore, taking hygienic measures is necessary for food safety and public health. |
| first_indexed | 2024-04-13T15:42:28Z |
| format | Article |
| id | doaj.art-c259f207574845da8ddc7f4cacd4dc4d |
| institution | Directory Open Access Journal |
| issn | 0026-704X 1846-4025 |
| language | English |
| last_indexed | 2024-04-13T15:42:28Z |
| publishDate | 2015-02-01 |
| publisher | Croatian Dairy Union |
| record_format | Article |
| series | Mljekarstvo |
| spelling | doaj.art-c259f207574845da8ddc7f4cacd4dc4d2022-12-22T02:41:06ZengCroatian Dairy UnionMljekarstvo0026-704X1846-40252015-02-01651263110.15567/mljekarstvo.2015.0104 Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR)Hayriye Yesim Can0Mehmet Elmalı1Alper Karagöz 2Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mustafa Kemal University, 31034 Hatay, Turkey Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mustafa Kemal University, 31034 Hatay, Turkey Microbiology Reference Laboratories Department, Public Health Institutes of Turkey, 06100 Ankara, Turkey Q fever is a widespread zoonotic disease that is caused by obligate intracellular bacteria, Coxiella burnetii. This study was planned to determine the prevalence of C. burnetii in cows’, goats’, and ewes’ bulk milk (BM) samples using PCR and to confirm positive results by DNA sequencing. A total of 150 BM samples (50 samples of each cows’, goats’, and ewes’ milk) collected from 15 randomly selected dairy farms in Hatay province were analyzed. The BM samples were taken between January 2012 and July 2013. Bacterial DNA was extracted directly from milk samples. Nine of the BM samples (6 %) were PCR positive; five from cows’ BM, two from ewes’ BM, and two from goats’ BM. Bacterial DNA was detected in 3 of 15 (20 %) dairy farms. Positive results were confirmed by DNA sequencing. The sequencing results of Coxiella DNA extracted from cows’, goats’, and ewes’ milk samples were consistent with the reference strain with 100 %, 99.7 %, 99.8 % homology, respectively. BM samples were found to be contaminated with C. burnetii; therefore, taking hygienic measures is necessary for food safety and public health.http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=197153 |
| spellingShingle | Hayriye Yesim Can Mehmet Elmalı Alper Karagöz Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR) Mljekarstvo |
| title | Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR) |
| title_full | Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR) |
| title_fullStr | Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR) |
| title_full_unstemmed | Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR) |
| title_short | Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR) |
| title_sort | detection of coxiella burnetii in cows goats and ewes bulk milk samples using polymerase chain reaction pcr |
| url | http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=197153 |
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