Multiple σEcfG and NepR Proteins Are Involved in the General Stress Response in Methylobacterium extorquens.
In Alphaproteobacteria, the general stress response (GSR) is controlled by a conserved partner switch composed of the sigma factor σ(EcfG), its anti-sigma factor NepR and the anti-sigma factor antagonist PhyR. Many species possess paralogues of one or several components of the system, but their role...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4814048?pdf=render |
| _version_ | 1811206999156719616 |
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| author | Anne Francez-Charlot Julia Frunzke Judith Zingg Andreas Kaczmarczyk Julia A Vorholt |
| author_facet | Anne Francez-Charlot Julia Frunzke Judith Zingg Andreas Kaczmarczyk Julia A Vorholt |
| author_sort | Anne Francez-Charlot |
| collection | DOAJ |
| description | In Alphaproteobacteria, the general stress response (GSR) is controlled by a conserved partner switch composed of the sigma factor σ(EcfG), its anti-sigma factor NepR and the anti-sigma factor antagonist PhyR. Many species possess paralogues of one or several components of the system, but their roles remain largely elusive. Among Alphaproteobacteria that have been genome-sequenced so far, the genus Methylobacterium possesses the largest number of σ(EcfG) proteins. Here, we analyzed the six σ(EcfG) paralogues of Methylobacterium extorquens AM1. We show that these sigma factors are not truly redundant, but instead exhibit major and minor contributions to stress resistance and GSR target gene expression. We identify distinct levels of regulation for the different sigma factors, as well as two NepR paralogues that interact with PhyR. Our results suggest that in M. extorquens AM1, ecfG and nepR paralogues have diverged in order to assume new roles that might allow integration of positive and negative feedback loops in the regulatory system. Comparison of the core elements of the GSR regulatory network in Methylobacterium species provides evidence for high plasticity and rapid evolution of the GSR core network in this genus. |
| first_indexed | 2024-04-12T03:57:35Z |
| format | Article |
| id | doaj.art-c25c7316414342b4bb8953d862e3b8a5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T03:57:35Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c25c7316414342b4bb8953d862e3b8a52022-12-22T03:48:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015251910.1371/journal.pone.0152519Multiple σEcfG and NepR Proteins Are Involved in the General Stress Response in Methylobacterium extorquens.Anne Francez-CharlotJulia FrunzkeJudith ZinggAndreas KaczmarczykJulia A VorholtIn Alphaproteobacteria, the general stress response (GSR) is controlled by a conserved partner switch composed of the sigma factor σ(EcfG), its anti-sigma factor NepR and the anti-sigma factor antagonist PhyR. Many species possess paralogues of one or several components of the system, but their roles remain largely elusive. Among Alphaproteobacteria that have been genome-sequenced so far, the genus Methylobacterium possesses the largest number of σ(EcfG) proteins. Here, we analyzed the six σ(EcfG) paralogues of Methylobacterium extorquens AM1. We show that these sigma factors are not truly redundant, but instead exhibit major and minor contributions to stress resistance and GSR target gene expression. We identify distinct levels of regulation for the different sigma factors, as well as two NepR paralogues that interact with PhyR. Our results suggest that in M. extorquens AM1, ecfG and nepR paralogues have diverged in order to assume new roles that might allow integration of positive and negative feedback loops in the regulatory system. Comparison of the core elements of the GSR regulatory network in Methylobacterium species provides evidence for high plasticity and rapid evolution of the GSR core network in this genus.http://europepmc.org/articles/PMC4814048?pdf=render |
| spellingShingle | Anne Francez-Charlot Julia Frunzke Judith Zingg Andreas Kaczmarczyk Julia A Vorholt Multiple σEcfG and NepR Proteins Are Involved in the General Stress Response in Methylobacterium extorquens. PLoS ONE |
| title | Multiple σEcfG and NepR Proteins Are Involved in the General Stress Response in Methylobacterium extorquens. |
| title_full | Multiple σEcfG and NepR Proteins Are Involved in the General Stress Response in Methylobacterium extorquens. |
| title_fullStr | Multiple σEcfG and NepR Proteins Are Involved in the General Stress Response in Methylobacterium extorquens. |
| title_full_unstemmed | Multiple σEcfG and NepR Proteins Are Involved in the General Stress Response in Methylobacterium extorquens. |
| title_short | Multiple σEcfG and NepR Proteins Are Involved in the General Stress Response in Methylobacterium extorquens. |
| title_sort | multiple σecfg and nepr proteins are involved in the general stress response in methylobacterium extorquens |
| url | http://europepmc.org/articles/PMC4814048?pdf=render |
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