Combined effect of the entomopathogenic fungus Metarhizium robertsii and avermectins on the survival and immune response of Aedes aegypti larvae
Combination of insect pathogenic fungi and microbial metabolites is a prospective method for mosquito control. The effect of the entomopathogenic fungus Metarhizium robertsii J.F. Bischoff, S.A. Rehner & Humber and avermectins on the survival and physiological parameters of Aedes aegypti (Linnae...
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PeerJ Inc.
2019-10-01
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| author | Yuriy A. Noskov Olga V. Polenogova Olga N. Yaroslavtseva Olga E. Belevich Yuriy A. Yurchenko Ekaterina A. Chertkova Natalya A. Kryukova Vadim Yu Kryukov Viktor V. Glupov |
| author_facet | Yuriy A. Noskov Olga V. Polenogova Olga N. Yaroslavtseva Olga E. Belevich Yuriy A. Yurchenko Ekaterina A. Chertkova Natalya A. Kryukova Vadim Yu Kryukov Viktor V. Glupov |
| author_sort | Yuriy A. Noskov |
| collection | DOAJ |
| description | Combination of insect pathogenic fungi and microbial metabolites is a prospective method for mosquito control. The effect of the entomopathogenic fungus Metarhizium robertsii J.F. Bischoff, S.A. Rehner & Humber and avermectins on the survival and physiological parameters of Aedes aegypti (Linnaeus, 1762) larvae (dopamine concentration, glutathione S-transferase (GST), nonspecific esterases (EST), acid proteases, lysozyme-like, phenoloxidase (PO) activities) was studied. It is shown that the combination of these agents leads to a synergistic effect on mosquito mortality. Colonization of Ae. aegypti larvae by hyphal bodies following water inoculation with conidia is shown for the first time. The larvae affected by fungi are characterized by a decrease in PO and dopamine levels. In the initial stages of toxicosis and/or fungal infection (12 h posttreatment), increases in the activity of insect detoxifying enzymes (GST and EST) and acid proteases are observed after monotreatments, and these increases are suppressed after combined treatment with the fungus and avermectins. Lysozyme-like activity is also most strongly suppressed under combined treatment with the fungus and avermectins in the early stages posttreatment (12 h). Forty-eight hours posttreatment, we observe increases in GST, EST, acid proteases, and lysozyme-like activities under the influence of the fungus and/or avermectins. The larvae affected by avermectins accumulate lower levels of conidia than avermectin-free larvae. On the other hand, a burst of bacterial CFUs is observed under treatment with both the fungus and avermectins. We suggest that disturbance of the responses of the immune and detoxifying systems under the combined treatment and the development of opportunistic bacteria may be among the causes of the synergistic effect. |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:40:39Z |
| publishDate | 2019-10-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-c262c3eefa104c0ca639a7d649d9a3052023-12-03T10:51:37ZengPeerJ Inc.PeerJ2167-83592019-10-017e793110.7717/peerj.7931Combined effect of the entomopathogenic fungus Metarhizium robertsii and avermectins on the survival and immune response of Aedes aegypti larvaeYuriy A. Noskov0Olga V. Polenogova1Olga N. Yaroslavtseva2Olga E. Belevich3Yuriy A. Yurchenko4Ekaterina A. Chertkova5Natalya A. Kryukova6Vadim Yu Kryukov7Viktor V. Glupov8Institute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk, RussiaCombination of insect pathogenic fungi and microbial metabolites is a prospective method for mosquito control. The effect of the entomopathogenic fungus Metarhizium robertsii J.F. Bischoff, S.A. Rehner & Humber and avermectins on the survival and physiological parameters of Aedes aegypti (Linnaeus, 1762) larvae (dopamine concentration, glutathione S-transferase (GST), nonspecific esterases (EST), acid proteases, lysozyme-like, phenoloxidase (PO) activities) was studied. It is shown that the combination of these agents leads to a synergistic effect on mosquito mortality. Colonization of Ae. aegypti larvae by hyphal bodies following water inoculation with conidia is shown for the first time. The larvae affected by fungi are characterized by a decrease in PO and dopamine levels. In the initial stages of toxicosis and/or fungal infection (12 h posttreatment), increases in the activity of insect detoxifying enzymes (GST and EST) and acid proteases are observed after monotreatments, and these increases are suppressed after combined treatment with the fungus and avermectins. Lysozyme-like activity is also most strongly suppressed under combined treatment with the fungus and avermectins in the early stages posttreatment (12 h). Forty-eight hours posttreatment, we observe increases in GST, EST, acid proteases, and lysozyme-like activities under the influence of the fungus and/or avermectins. The larvae affected by avermectins accumulate lower levels of conidia than avermectin-free larvae. On the other hand, a burst of bacterial CFUs is observed under treatment with both the fungus and avermectins. We suggest that disturbance of the responses of the immune and detoxifying systems under the combined treatment and the development of opportunistic bacteria may be among the causes of the synergistic effect.https://peerj.com/articles/7931.pdfSynergismLysozyme-like activityMosquitoAcid proteasesPhenoloxidaseDopamine |
| spellingShingle | Yuriy A. Noskov Olga V. Polenogova Olga N. Yaroslavtseva Olga E. Belevich Yuriy A. Yurchenko Ekaterina A. Chertkova Natalya A. Kryukova Vadim Yu Kryukov Viktor V. Glupov Combined effect of the entomopathogenic fungus Metarhizium robertsii and avermectins on the survival and immune response of Aedes aegypti larvae PeerJ Synergism Lysozyme-like activity Mosquito Acid proteases Phenoloxidase Dopamine |
| title | Combined effect of the entomopathogenic fungus Metarhizium robertsii and avermectins on the survival and immune response of Aedes aegypti larvae |
| title_full | Combined effect of the entomopathogenic fungus Metarhizium robertsii and avermectins on the survival and immune response of Aedes aegypti larvae |
| title_fullStr | Combined effect of the entomopathogenic fungus Metarhizium robertsii and avermectins on the survival and immune response of Aedes aegypti larvae |
| title_full_unstemmed | Combined effect of the entomopathogenic fungus Metarhizium robertsii and avermectins on the survival and immune response of Aedes aegypti larvae |
| title_short | Combined effect of the entomopathogenic fungus Metarhizium robertsii and avermectins on the survival and immune response of Aedes aegypti larvae |
| title_sort | combined effect of the entomopathogenic fungus metarhizium robertsii and avermectins on the survival and immune response of aedes aegypti larvae |
| topic | Synergism Lysozyme-like activity Mosquito Acid proteases Phenoloxidase Dopamine |
| url | https://peerj.com/articles/7931.pdf |
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