Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study
Introduction: Root canal sealers with good sealing ability and antimicrobial activity are desired to entomb the surviving microorganisms. Aim: Aim of the present study is to evaluate the antimicrobial effect of different sealers mixed with antibiotics. Materials and Methods: Sixty extracted pre...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2016-04-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/7645/17228_CE(EK)_F(AK)_PF1(EKAK)_PFA(AK)_PF2(PAG).pdf |
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| author | Amulya Vanapatla Harikumar Vemisetty Rajani Punna Chandrasekhar Veeramachineni Ravichandra Polavarapu Venkata Jaya Nagendra Krishna Muppala Ramkiran Dandolu |
| author_facet | Amulya Vanapatla Harikumar Vemisetty Rajani Punna Chandrasekhar Veeramachineni Ravichandra Polavarapu Venkata Jaya Nagendra Krishna Muppala Ramkiran Dandolu |
| author_sort | Amulya Vanapatla |
| collection | DOAJ |
| description | Introduction: Root canal sealers with good sealing ability
and antimicrobial activity are desired to entomb the surviving
microorganisms.
Aim: Aim of the present study is to evaluate the antimicrobial
effect of different sealers mixed with antibiotics.
Materials and Methods: Sixty extracted premolars were taken
and were cut coronally & apically such that 7mm of tooth
specimen was prepared. They were sterilized by autoclaving,
inoculated with E.faecalis and incubated for three weeks. The
specimens were divided into six groups of 10 each. Group 1 –
ZnOE, Group 2 – ZnOE +TAM, Group 3 – Apexit Plus, Group 4 –
Apexit Plus +TAM, Group 5 – AH Plus, Group 6 – AH Plus + TAM.
Bacterial growth in the each specimen was calculated before &
after sealer application and noted as initial & final colony count.
Antimicrobial effect of each sealer was measured by calculating
the Percentage Reduction in Colony Count (%RCC). One way
analysis of variance and post hoc tests were used for statistical
analysis.
Results: ZnOE + TAM group showed maximum antibacterial
effect among the sealers tested and AH Plus sealer showed
least antimicrobial effect.
Conclusion: When the sealers were mixed with triple antibiotic
mixture the antimicrobial effect was increased significantly. |
| first_indexed | 2024-12-19T19:23:37Z |
| format | Article |
| id | doaj.art-c26419c1124246629f41651de1bdec86 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-19T19:23:37Z |
| publishDate | 2016-04-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-c26419c1124246629f41651de1bdec862022-12-21T20:08:54ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-04-01104ZC69ZC7210.7860/JCDR/2016/17228.7645Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro StudyAmulya Vanapatla0Harikumar Vemisetty1Rajani Punna2Chandrasekhar Veeramachineni3Ravichandra Polavarapu Venkata4Jaya Nagendra Krishna Muppala5Ramkiran Dandolu6Senior Lecturer, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences, Narketpally, Nalgonda, Telangana, India. Professor and HOD, Department of Conservative dentistry and Endodontics, Kamineni Institute of Dental Sciences, Narketpally, Nalgonda, Telangana, India.Senior Lecturer, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences, Narketpally, Nalgonda, Telangana, India.Professor and HOD, Department of Conservative Dentistry and Endodontics, Mamata Dental College, Khammam, Telangana, India.Professor, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences, Narketpally, Nalgonda, Telangana, India.Reader, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences, Narketpally, Nalgonda, Telangana, India.Reader, Department of Conservative Dentistry and Endodontics, Kamineni Institute of Dental Sciences, Narketpally, Nalgonda, Telangana, India.Introduction: Root canal sealers with good sealing ability and antimicrobial activity are desired to entomb the surviving microorganisms. Aim: Aim of the present study is to evaluate the antimicrobial effect of different sealers mixed with antibiotics. Materials and Methods: Sixty extracted premolars were taken and were cut coronally & apically such that 7mm of tooth specimen was prepared. They were sterilized by autoclaving, inoculated with E.faecalis and incubated for three weeks. The specimens were divided into six groups of 10 each. Group 1 – ZnOE, Group 2 – ZnOE +TAM, Group 3 – Apexit Plus, Group 4 – Apexit Plus +TAM, Group 5 – AH Plus, Group 6 – AH Plus + TAM. Bacterial growth in the each specimen was calculated before & after sealer application and noted as initial & final colony count. Antimicrobial effect of each sealer was measured by calculating the Percentage Reduction in Colony Count (%RCC). One way analysis of variance and post hoc tests were used for statistical analysis. Results: ZnOE + TAM group showed maximum antibacterial effect among the sealers tested and AH Plus sealer showed least antimicrobial effect. Conclusion: When the sealers were mixed with triple antibiotic mixture the antimicrobial effect was increased significantly.https://jcdr.net/articles/PDF/7645/17228_CE(EK)_F(AK)_PF1(EKAK)_PFA(AK)_PF2(PAG).pdfapexit plus sealerah plus sealertriple antibiotic mixturezinc oxide eugenol sealer |
| spellingShingle | Amulya Vanapatla Harikumar Vemisetty Rajani Punna Chandrasekhar Veeramachineni Ravichandra Polavarapu Venkata Jaya Nagendra Krishna Muppala Ramkiran Dandolu Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study Journal of Clinical and Diagnostic Research apexit plus sealer ah plus sealer triple antibiotic mixture zinc oxide eugenol sealer |
| title | Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study |
| title_full | Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study |
| title_fullStr | Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study |
| title_full_unstemmed | Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study |
| title_short | Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study |
| title_sort | comparative evaluation of antimicrobial effect of three endodontic sealers with and without antibiotics an in vitro study |
| topic | apexit plus sealer ah plus sealer triple antibiotic mixture zinc oxide eugenol sealer |
| url | https://jcdr.net/articles/PDF/7645/17228_CE(EK)_F(AK)_PF1(EKAK)_PFA(AK)_PF2(PAG).pdf |
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