Tiansi Liquid Modulates Gut Microbiota Composition and Tryptophan–Kynurenine Metabolism in Rats with Hydrocortisone-Induced Depression
Tiansi Liquid is a traditional Chinese herbal medicine used to treat depression; however, the underlying mechanisms remain unclear. Here, we examined the effect of Tiansi Liquid in a rat model of hydrocortisone-induced depression using behavioral testing, 16S rRNA high-throughput pyrosequencing and...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-10-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/23/11/2832 |
| _version_ | 1828325428436140032 |
|---|---|
| author | Dan Cheng Hongsheng Chang Suya Ma Jian Guo Gaimei She Feilong Zhang Lingling Li Xinjie Li Yi Lu |
| author_facet | Dan Cheng Hongsheng Chang Suya Ma Jian Guo Gaimei She Feilong Zhang Lingling Li Xinjie Li Yi Lu |
| author_sort | Dan Cheng |
| collection | DOAJ |
| description | Tiansi Liquid is a traditional Chinese herbal medicine used to treat depression; however, the underlying mechanisms remain unclear. Here, we examined the effect of Tiansi Liquid in a rat model of hydrocortisone-induced depression using behavioral testing, 16S rRNA high-throughput pyrosequencing and high-performance liquid chromatography-mass spectrometry-based metabolomics of the tryptophan (TRP)⁻kynurenine (KYN) pathway. Tiansi Liquid significantly improved the sucrose preference and exploratory behavior of the depressive rats. The richness of intestinal mucosa samples from the model (depressive) group tended to be higher than that from the control group, while the richness was higher in the Tiansi Liquid-treated group than in the model group. Tiansi Liquid increased the relative abundance of some microbiota (<i>Ruminococcaceae</i>, <i>Lactococcus</i>, <i>Lactobacillus</i>, <i>Lachnospiraceae_NK4A136_group</i>). Metabolomics showed that Tiansi Liquid reduced the levels of tryptophan 2,3 dioxygenase, indoleamine 2,3-dioxygenase, quinoline and the KYN/TRP ratio, while increasing kynurenic acid and 5-HT levels. Correlation analysis revealed a negative relationship between the relative abundance of the <i>Lachnospiraceae_NK4A136_group</i> and quinoline content. Collectively, these findings suggest that Tiansi Liquid ameliorates depressive symptoms in rats by modulating the gut microbiota composition and metabolites in the TRP⁻KYN pathway. |
| first_indexed | 2024-04-13T19:21:43Z |
| format | Article |
| id | doaj.art-c265f2b0c0ca4540963613a276d11a16 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T19:21:43Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c265f2b0c0ca4540963613a276d11a162022-12-22T02:33:30ZengMDPI AGMolecules1420-30492018-10-012311283210.3390/molecules23112832molecules23112832Tiansi Liquid Modulates Gut Microbiota Composition and Tryptophan–Kynurenine Metabolism in Rats with Hydrocortisone-Induced DepressionDan Cheng0Hongsheng Chang1Suya Ma2Jian Guo3Gaimei She4Feilong Zhang5Lingling Li6Xinjie Li7Yi Lu8School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaTiansi Liquid is a traditional Chinese herbal medicine used to treat depression; however, the underlying mechanisms remain unclear. Here, we examined the effect of Tiansi Liquid in a rat model of hydrocortisone-induced depression using behavioral testing, 16S rRNA high-throughput pyrosequencing and high-performance liquid chromatography-mass spectrometry-based metabolomics of the tryptophan (TRP)⁻kynurenine (KYN) pathway. Tiansi Liquid significantly improved the sucrose preference and exploratory behavior of the depressive rats. The richness of intestinal mucosa samples from the model (depressive) group tended to be higher than that from the control group, while the richness was higher in the Tiansi Liquid-treated group than in the model group. Tiansi Liquid increased the relative abundance of some microbiota (<i>Ruminococcaceae</i>, <i>Lactococcus</i>, <i>Lactobacillus</i>, <i>Lachnospiraceae_NK4A136_group</i>). Metabolomics showed that Tiansi Liquid reduced the levels of tryptophan 2,3 dioxygenase, indoleamine 2,3-dioxygenase, quinoline and the KYN/TRP ratio, while increasing kynurenic acid and 5-HT levels. Correlation analysis revealed a negative relationship between the relative abundance of the <i>Lachnospiraceae_NK4A136_group</i> and quinoline content. Collectively, these findings suggest that Tiansi Liquid ameliorates depressive symptoms in rats by modulating the gut microbiota composition and metabolites in the TRP⁻KYN pathway.https://www.mdpi.com/1420-3049/23/11/2832Tiansi Liquidantidepressant effects16S rRNA high-throughput pyrosequencinggut microbiotahigh-performance liquid chromatography-mass spectrometrytryptophan–kynurenine pathway |
| spellingShingle | Dan Cheng Hongsheng Chang Suya Ma Jian Guo Gaimei She Feilong Zhang Lingling Li Xinjie Li Yi Lu Tiansi Liquid Modulates Gut Microbiota Composition and Tryptophan–Kynurenine Metabolism in Rats with Hydrocortisone-Induced Depression Molecules Tiansi Liquid antidepressant effects 16S rRNA high-throughput pyrosequencing gut microbiota high-performance liquid chromatography-mass spectrometry tryptophan–kynurenine pathway |
| title | Tiansi Liquid Modulates Gut Microbiota Composition and Tryptophan–Kynurenine Metabolism in Rats with Hydrocortisone-Induced Depression |
| title_full | Tiansi Liquid Modulates Gut Microbiota Composition and Tryptophan–Kynurenine Metabolism in Rats with Hydrocortisone-Induced Depression |
| title_fullStr | Tiansi Liquid Modulates Gut Microbiota Composition and Tryptophan–Kynurenine Metabolism in Rats with Hydrocortisone-Induced Depression |
| title_full_unstemmed | Tiansi Liquid Modulates Gut Microbiota Composition and Tryptophan–Kynurenine Metabolism in Rats with Hydrocortisone-Induced Depression |
| title_short | Tiansi Liquid Modulates Gut Microbiota Composition and Tryptophan–Kynurenine Metabolism in Rats with Hydrocortisone-Induced Depression |
| title_sort | tiansi liquid modulates gut microbiota composition and tryptophan kynurenine metabolism in rats with hydrocortisone induced depression |
| topic | Tiansi Liquid antidepressant effects 16S rRNA high-throughput pyrosequencing gut microbiota high-performance liquid chromatography-mass spectrometry tryptophan–kynurenine pathway |
| url | https://www.mdpi.com/1420-3049/23/11/2832 |
| work_keys_str_mv | AT dancheng tiansiliquidmodulatesgutmicrobiotacompositionandtryptophankynureninemetabolisminratswithhydrocortisoneinduceddepression AT hongshengchang tiansiliquidmodulatesgutmicrobiotacompositionandtryptophankynureninemetabolisminratswithhydrocortisoneinduceddepression AT suyama tiansiliquidmodulatesgutmicrobiotacompositionandtryptophankynureninemetabolisminratswithhydrocortisoneinduceddepression AT jianguo tiansiliquidmodulatesgutmicrobiotacompositionandtryptophankynureninemetabolisminratswithhydrocortisoneinduceddepression AT gaimeishe tiansiliquidmodulatesgutmicrobiotacompositionandtryptophankynureninemetabolisminratswithhydrocortisoneinduceddepression AT feilongzhang tiansiliquidmodulatesgutmicrobiotacompositionandtryptophankynureninemetabolisminratswithhydrocortisoneinduceddepression AT linglingli tiansiliquidmodulatesgutmicrobiotacompositionandtryptophankynureninemetabolisminratswithhydrocortisoneinduceddepression AT xinjieli tiansiliquidmodulatesgutmicrobiotacompositionandtryptophankynureninemetabolisminratswithhydrocortisoneinduceddepression AT yilu tiansiliquidmodulatesgutmicrobiotacompositionandtryptophankynureninemetabolisminratswithhydrocortisoneinduceddepression |