Inhibitory Activity of Levofloxacin against MDR Staphylococcus aureus and Pseudomonas aeruginosa Clinical Isolates
Introduction: Staphylococcus aureus and Pseudomonas aeruginosa are the most dangerous and important species among their genus. These bacteria are often resistant to many classes of antimicrobial agents; which make difficulties in selecting appropriate drug to treat infections. Mult...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Indonesian |
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Faculty of Pharmacy, Universitas Airlangga
2019-07-01
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| Series: | Jurnal Farmasi dan Ilmu Kefarmasian Indonesia |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/JFIKI/article/view/13024 |
| _version_ | 1818871069874847744 |
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| author | Lisa Nathalie Lindawati Alimsardjono Isnaeni Isnaeni |
| author_facet | Lisa Nathalie Lindawati Alimsardjono Isnaeni Isnaeni |
| author_sort | Lisa Nathalie |
| collection | DOAJ |
| description | Introduction: Staphylococcus aureus and Pseudomonas aeruginosa are the most dangerous and important species among their genus. These bacteria are often resistant to many classes of antimicrobial agents; which make difficulties in selecting appropriate drug to treat infections. Multidrug-resistance occurs readily in hospitals for which antimicrobials agents were used widely. Objective: The aims of this study was to determine minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) of levofloxacin against 22 multidrug resistant- clinical (MDR) strains of Staphylococcus aureus and Pseudomonas aeruginosa isolated from patients pus and urine in hospital. Methods: Determination of the MIC was performed by macro-dilution broth assay as recommended by Clinical and Laboratory Standards Institute (CLSI), while the MBC was determined one-step further after the MIC determination. Results: It was found that MIC of the levofloxacin were (0.3 ± 0.0) - >0.5 µg/mL and (0.2 ± 0.1) - (1.0 ±0.0)µg/mL against S. aureus from pus and urine, respectively. In addition, higher MICs were yielded against P. aeruginosa, (1.0 ± 0.0) - >8.0 µg/mL and (0.7 ± 0.3) - (3.0 ± 1.2) µg/mL for pus and urine isolates respectively. Similar to MICs, the MBCs against P. aeruginosa were higher than S. aureus, (0.6 ± 0.0) - > 4.0 µg/mL and (0.3 ± 0.0) - >8.0 µg/mL isolated from pus and urine respectively, (2.0 ± 0.6) - > 8.0 µg/mL and (3.0 ± 1.2) - >7.0 µg/mL against P. aeruginosa from pus and urine respectively. Conclusion: The levofloxacin was still susceptible as bacteriostatic against isolates from both body fluids, but not bactericidal towards all isolates. |
| first_indexed | 2024-12-19T12:17:04Z |
| format | Article |
| id | doaj.art-c2682ff1955a4d89aa4a2196f306f1db |
| institution | Directory Open Access Journal |
| issn | 2406-9388 2580-8303 |
| language | Indonesian |
| last_indexed | 2024-12-19T12:17:04Z |
| publishDate | 2019-07-01 |
| publisher | Faculty of Pharmacy, Universitas Airlangga |
| record_format | Article |
| series | Jurnal Farmasi dan Ilmu Kefarmasian Indonesia |
| spelling | doaj.art-c2682ff1955a4d89aa4a2196f306f1db2022-12-21T20:21:56ZindFaculty of Pharmacy, Universitas AirlanggaJurnal Farmasi dan Ilmu Kefarmasian Indonesia2406-93882580-83032019-07-0161253110.20473/jfiki.v6i12019.25-317117Inhibitory Activity of Levofloxacin against MDR Staphylococcus aureus and Pseudomonas aeruginosa Clinical IsolatesLisa Nathalie0Lindawati Alimsardjono1Isnaeni Isnaeni2Departemen Kimia Farmasi, Fakultas Farmasi, Universitas AirlanggaDepartemen Mikrobiologi, Fakultas Kedokteran, Universitas AirlanggaDepartemen Kimia Farmasi, Fakultas Farmasi, Universitas AirlanggaIntroduction: Staphylococcus aureus and Pseudomonas aeruginosa are the most dangerous and important species among their genus. These bacteria are often resistant to many classes of antimicrobial agents; which make difficulties in selecting appropriate drug to treat infections. Multidrug-resistance occurs readily in hospitals for which antimicrobials agents were used widely. Objective: The aims of this study was to determine minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) of levofloxacin against 22 multidrug resistant- clinical (MDR) strains of Staphylococcus aureus and Pseudomonas aeruginosa isolated from patients pus and urine in hospital. Methods: Determination of the MIC was performed by macro-dilution broth assay as recommended by Clinical and Laboratory Standards Institute (CLSI), while the MBC was determined one-step further after the MIC determination. Results: It was found that MIC of the levofloxacin were (0.3 ± 0.0) - >0.5 µg/mL and (0.2 ± 0.1) - (1.0 ±0.0)µg/mL against S. aureus from pus and urine, respectively. In addition, higher MICs were yielded against P. aeruginosa, (1.0 ± 0.0) - >8.0 µg/mL and (0.7 ± 0.3) - (3.0 ± 1.2) µg/mL for pus and urine isolates respectively. Similar to MICs, the MBCs against P. aeruginosa were higher than S. aureus, (0.6 ± 0.0) - > 4.0 µg/mL and (0.3 ± 0.0) - >8.0 µg/mL isolated from pus and urine respectively, (2.0 ± 0.6) - > 8.0 µg/mL and (3.0 ± 1.2) - >7.0 µg/mL against P. aeruginosa from pus and urine respectively. Conclusion: The levofloxacin was still susceptible as bacteriostatic against isolates from both body fluids, but not bactericidal towards all isolates.https://e-journal.unair.ac.id/JFIKI/article/view/13024levofloxacin, mdr, pseudomonas aeruginosa, staphylococcus aureus |
| spellingShingle | Lisa Nathalie Lindawati Alimsardjono Isnaeni Isnaeni Inhibitory Activity of Levofloxacin against MDR Staphylococcus aureus and Pseudomonas aeruginosa Clinical Isolates Jurnal Farmasi dan Ilmu Kefarmasian Indonesia levofloxacin, mdr, pseudomonas aeruginosa, staphylococcus aureus |
| title | Inhibitory Activity of Levofloxacin against MDR Staphylococcus aureus and Pseudomonas aeruginosa Clinical Isolates |
| title_full | Inhibitory Activity of Levofloxacin against MDR Staphylococcus aureus and Pseudomonas aeruginosa Clinical Isolates |
| title_fullStr | Inhibitory Activity of Levofloxacin against MDR Staphylococcus aureus and Pseudomonas aeruginosa Clinical Isolates |
| title_full_unstemmed | Inhibitory Activity of Levofloxacin against MDR Staphylococcus aureus and Pseudomonas aeruginosa Clinical Isolates |
| title_short | Inhibitory Activity of Levofloxacin against MDR Staphylococcus aureus and Pseudomonas aeruginosa Clinical Isolates |
| title_sort | inhibitory activity of levofloxacin against mdr staphylococcus aureus and pseudomonas aeruginosa clinical isolates |
| topic | levofloxacin, mdr, pseudomonas aeruginosa, staphylococcus aureus |
| url | https://e-journal.unair.ac.id/JFIKI/article/view/13024 |
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