Evaluation of Tnf-α and Il-6 mRNAs expressions in visceral and subcutaneous adipose tissues of polycystic ovarian rats and effects of resveratrol
Objective(s): Some studies suggest that chronic low-grade inflammation is involved in insulin resistance in polycystic ovary syndrome (PCOS). This study assessed possible involvement of alteration in expression of two pro-inflammatory factors, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2018-02-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_10031_eacda6285bacb04d43afe2538fdd614d.pdf |
| _version_ | 1818753342622400512 |
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| author | Mahnaz Ghowsi Homayoun Khazali Sajjad Sisakhtnezhad |
| author_facet | Mahnaz Ghowsi Homayoun Khazali Sajjad Sisakhtnezhad |
| author_sort | Mahnaz Ghowsi |
| collection | DOAJ |
| description | Objective(s): Some studies suggest that chronic low-grade inflammation is involved in insulin resistance in polycystic ovary syndrome (PCOS). This study assessed possible involvement of alteration in expression of two pro-inflammatory factors, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in adipose tissues of PCOS rats in the impairment of insulin actions. Also, effects of resveratrol as an anti-inflammatory agent were investigated. Materials and Methods: Fifteen female Wistar rats (21 days old) were divided into three groups (n=5): Ι) Control, П) PCO-model-saline: served as PCOS rats and to induce PCOS, received subcutaneously testosterone enanthate 1 mg/100 g body weight subcutaneously for 35 days, Ш) PCO-model-resveratrol, after receiving testosterone, received resveratrol 10 mg/kg intraperitoneally for 28 days. The expression of Tnf-α and Il-6 mRNAs in adipose tissues was determined by the qRT-PCR method. Results: The Il-6 mRNA expression in the visceral adipose tissue of PCOS rats was increased in comparison to controls (P |
| first_indexed | 2024-12-18T05:05:50Z |
| format | Article |
| id | doaj.art-c26b21131c1d420caf456798404595c6 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-18T05:05:50Z |
| publishDate | 2018-02-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-c26b21131c1d420caf456798404595c62022-12-21T21:20:00ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742018-02-0121216517410.22038/ijbms.2017.24801.616710031Evaluation of Tnf-α and Il-6 mRNAs expressions in visceral and subcutaneous adipose tissues of polycystic ovarian rats and effects of resveratrolMahnaz Ghowsi0Homayoun Khazali1Sajjad Sisakhtnezhad2Department of Physiology, Faculty of Biological Sciences and Technology, Shahid Beheshti University, Tehran, IranDepartment of Physiology, Faculty of Biological Sciences and Technology, Shahid Beheshti University, Tehran, IranDepartment of Biology, Faculty of Sciences, Razi University, Kermanshah, IranObjective(s): Some studies suggest that chronic low-grade inflammation is involved in insulin resistance in polycystic ovary syndrome (PCOS). This study assessed possible involvement of alteration in expression of two pro-inflammatory factors, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in adipose tissues of PCOS rats in the impairment of insulin actions. Also, effects of resveratrol as an anti-inflammatory agent were investigated. Materials and Methods: Fifteen female Wistar rats (21 days old) were divided into three groups (n=5): Ι) Control, П) PCO-model-saline: served as PCOS rats and to induce PCOS, received subcutaneously testosterone enanthate 1 mg/100 g body weight subcutaneously for 35 days, Ш) PCO-model-resveratrol, after receiving testosterone, received resveratrol 10 mg/kg intraperitoneally for 28 days. The expression of Tnf-α and Il-6 mRNAs in adipose tissues was determined by the qRT-PCR method. Results: The Il-6 mRNA expression in the visceral adipose tissue of PCOS rats was increased in comparison to controls (Phttp://ijbms.mums.ac.ir/article_10031_eacda6285bacb04d43afe2538fdd614d.pdfAdipose tissueIL-6Polycystic ovary –syndromeRatResveratrolTNF-α |
| spellingShingle | Mahnaz Ghowsi Homayoun Khazali Sajjad Sisakhtnezhad Evaluation of Tnf-α and Il-6 mRNAs expressions in visceral and subcutaneous adipose tissues of polycystic ovarian rats and effects of resveratrol Iranian Journal of Basic Medical Sciences Adipose tissue IL-6 Polycystic ovary – syndrome Rat Resveratrol TNF-α |
| title | Evaluation of Tnf-α and Il-6 mRNAs expressions in visceral and subcutaneous adipose tissues of polycystic ovarian rats and effects of resveratrol |
| title_full | Evaluation of Tnf-α and Il-6 mRNAs expressions in visceral and subcutaneous adipose tissues of polycystic ovarian rats and effects of resveratrol |
| title_fullStr | Evaluation of Tnf-α and Il-6 mRNAs expressions in visceral and subcutaneous adipose tissues of polycystic ovarian rats and effects of resveratrol |
| title_full_unstemmed | Evaluation of Tnf-α and Il-6 mRNAs expressions in visceral and subcutaneous adipose tissues of polycystic ovarian rats and effects of resveratrol |
| title_short | Evaluation of Tnf-α and Il-6 mRNAs expressions in visceral and subcutaneous adipose tissues of polycystic ovarian rats and effects of resveratrol |
| title_sort | evaluation of tnf α and il 6 mrnas expressions in visceral and subcutaneous adipose tissues of polycystic ovarian rats and effects of resveratrol |
| topic | Adipose tissue IL-6 Polycystic ovary – syndrome Rat Resveratrol TNF-α |
| url | http://ijbms.mums.ac.ir/article_10031_eacda6285bacb04d43afe2538fdd614d.pdf |
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