Innovative multiplex and its evaluation for effective genotyping of wild cherry
Trees from the family Rosaceae play an important role in forest and agricultural ecosystems. Therefore, they are often an object of interest for both forest and horticultural tree breeders. Here, we present the utilization of an effective microsatellite (SSRs) genotyping method for wild cherry (Prun...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Finnish Society of Forest Science
2017-06-01
|
| Series: | Silva Fennica |
| Subjects: |
| _version_ | 1819052228444422144 |
|---|---|
| author | Jiří Korecký Jan Bílý Petr Sedlák Milan Lstibůrek |
| author_facet | Jiří Korecký Jan Bílý Petr Sedlák Milan Lstibůrek |
| author_sort | Jiří Korecký |
| collection | DOAJ |
| description | Trees from the family Rosaceae play an important role in forest and agricultural ecosystems. Therefore, they are often an object of interest for both forest and horticultural tree breeders. Here, we present the utilization of an effective microsatellite (SSRs) genotyping method for wild cherry (Prunus avium L.) and verified the discriminatory power of the presented multiplex by genotyping 48 genetically distinctive individuals (plus-trees). Concerned loci were previously proven to be cross-compatible among various cultivars of cherry, hence, the method could have a broader utilization beyond to the field of forestry. Our technique is based on post-PCR processing of 15 polymorphic SSRs loci amplified in three multiplex reactions with fluorescently labeled primers (6-FAM, VIC, PET and NED). All PCR products could be pooled and analyzed simultaneously (pseudo 15-plex). In order to make this approach feasible, we redefined sequences of several primers. Thus, utilizing modified primers provides non-overlapping amplicons of each fluorescent dye. |
| first_indexed | 2024-12-21T12:16:30Z |
| format | Article |
| id | doaj.art-c26bf5debbf8489a90cbdc56e4cb0441 |
| institution | Directory Open Access Journal |
| issn | 2242-4075 2242-4075 |
| language | English |
| last_indexed | 2024-12-21T12:16:30Z |
| publishDate | 2017-06-01 |
| publisher | Finnish Society of Forest Science |
| record_format | Article |
| series | Silva Fennica |
| spelling | doaj.art-c26bf5debbf8489a90cbdc56e4cb04412022-12-21T19:04:26ZengFinnish Society of Forest ScienceSilva Fennica2242-40752242-40752017-06-0151310.14214/sf.5644Innovative multiplex and its evaluation for effective genotyping of wild cherryJiří Korecký0https://orcid.org/0000-0001-7859-1750Jan Bílý1https://orcid.org/0000-0001-5794-0907Petr Sedlák2https://orcid.org/0000-0001-8016-8900Milan Lstibůrek3https://orcid.org/0000-0002-6304-6669Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Kamýcká 1176, 165 21, Prague, Czech RepublicFaculty of Forestry and Wood Sciences, Czech University of Life Sciences, Kamýcká 1176, 165 21, Prague, Czech RepublicFaculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Kamýcká 129, 165 21, Prague, Czech RepublicFaculty of Forestry and Wood Sciences, Czech University of Life Sciences, Kamýcká 1176, 165 21, Prague, Czech RepublicTrees from the family Rosaceae play an important role in forest and agricultural ecosystems. Therefore, they are often an object of interest for both forest and horticultural tree breeders. Here, we present the utilization of an effective microsatellite (SSRs) genotyping method for wild cherry (Prunus avium L.) and verified the discriminatory power of the presented multiplex by genotyping 48 genetically distinctive individuals (plus-trees). Concerned loci were previously proven to be cross-compatible among various cultivars of cherry, hence, the method could have a broader utilization beyond to the field of forestry. Our technique is based on post-PCR processing of 15 polymorphic SSRs loci amplified in three multiplex reactions with fluorescently labeled primers (6-FAM, VIC, PET and NED). All PCR products could be pooled and analyzed simultaneously (pseudo 15-plex). In order to make this approach feasible, we redefined sequences of several primers. Thus, utilizing modified primers provides non-overlapping amplicons of each fluorescent dye.prunus aviumssrs; microsatellitespseudo 15-plexone-run genotypinggenetic structure |
| spellingShingle | Jiří Korecký Jan Bílý Petr Sedlák Milan Lstibůrek Innovative multiplex and its evaluation for effective genotyping of wild cherry Silva Fennica prunus avium ssrs; microsatellites pseudo 15-plex one-run genotyping genetic structure |
| title | Innovative multiplex and its evaluation for effective genotyping of wild cherry |
| title_full | Innovative multiplex and its evaluation for effective genotyping of wild cherry |
| title_fullStr | Innovative multiplex and its evaluation for effective genotyping of wild cherry |
| title_full_unstemmed | Innovative multiplex and its evaluation for effective genotyping of wild cherry |
| title_short | Innovative multiplex and its evaluation for effective genotyping of wild cherry |
| title_sort | innovative multiplex and its evaluation for effective genotyping of wild cherry |
| topic | prunus avium ssrs; microsatellites pseudo 15-plex one-run genotyping genetic structure |
| work_keys_str_mv | AT jirikorecky innovativemultiplexanditsevaluationforeffectivegenotypingofwildcherry AT janbily innovativemultiplexanditsevaluationforeffectivegenotypingofwildcherry AT petrsedlak innovativemultiplexanditsevaluationforeffectivegenotypingofwildcherry AT milanlstiburek innovativemultiplexanditsevaluationforeffectivegenotypingofwildcherry |