Association of leptin, toll-like receptor 4, and chemokine receptor of interleukin 8 C-X-C motif single nucleotide polymorphisms with fertility traits in Czech Fleckvieh cattle
Objective The use of genetic markers can help to enhance reproduction in cattle, which is a very important trait for profitability in dairy production systems. This study evaluated the association between genotypes of leptin (LEP), toll-like receptor 4 (TLR4), and chemokine receptor of interleukin 8...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2018-11-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-17-0900.pdf |
| _version_ | 1828837129271115776 |
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| author | Katerina Jecminkova Uwe Müller Jitka Kyselova Zuzana Sztankoova Ludmila Zavadilova Miloslava Stipkova Ivan Majzlik |
| author_facet | Katerina Jecminkova Uwe Müller Jitka Kyselova Zuzana Sztankoova Ludmila Zavadilova Miloslava Stipkova Ivan Majzlik |
| author_sort | Katerina Jecminkova |
| collection | DOAJ |
| description | Objective The use of genetic markers can help to enhance reproduction in cattle, which is a very important trait for profitability in dairy production systems. This study evaluated the association between genotypes of leptin (LEP), toll-like receptor 4 (TLR4), and chemokine receptor of interleukin 8 C-X-C motif (CXCR1) genes and fertility traits in Czech Fleckvieh cattle. Methods Phenotypic data from 786 Czech Fleckvieh cows raised on 5 farms in the Czech Republic were used, along with information from the 1st three parities. To determine genotype, the polymerase chain reaction– restriction fragment length polymorphism method was used. Results Except for LEP g.-963C>T, all studied genotype frequencies of single nucleotide polymorphisms (SNPs) were distributed according to the Hardy-Weinberg equilibrium. Two LEP SNPs (g.-963C>T and c.357C>T) were associated with the age at the 1st calving, days open (DO), pregnancy rate after 1st service (PR), and calving interval (CLI). In LEP g.-963C>T the TT genotype heifers firstly calved 24 days earlier than CC genotype and the CT genotype cow showed a tendency for shorter DO and higher PR. In LEP c.357C>T we observed longer CLI and DO period in TT cows. In general, we can propose the TT genotype of g.-963C>T as favorable and the TT genotype of c.357C>T as unfavorable for a cow’s fertility. Heterozygotes in TLR4 c.-226C>G were significantly associated with shorter CLI, and presented a nonsignificant tendency to be associated with higher PR. In CXCR1 c.777 C>G, we did not observe any relationship of this SNP with reproduction. Conclusion Overall, the results showed that LEP could be an effective marker for improving reproduction in Czech Fleckvieh cattle. This study also provides novel insights into the relationship between TLR4 and CXCR1 SNPs and reproduction in dual-purpose cattle. |
| first_indexed | 2024-12-12T18:35:52Z |
| format | Article |
| id | doaj.art-c271dc0fe3c64bc987705108ddc924a8 |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-12T18:35:52Z |
| publishDate | 2018-11-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-c271dc0fe3c64bc987705108ddc924a82022-12-22T00:15:48ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172018-11-0131111721172810.5713/ajas.17.090023982Association of leptin, toll-like receptor 4, and chemokine receptor of interleukin 8 C-X-C motif single nucleotide polymorphisms with fertility traits in Czech Fleckvieh cattleKaterina Jecminkova0Uwe Müller1Jitka Kyselova2Zuzana Sztankoova3Ludmila Zavadilova4Miloslava Stipkova5Ivan Majzlik6 Institute of Animal Science, Prague 10400, Czech Republic Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt-Universität zu Berlin, Berlin 100 99, Germany Institute of Animal Science, Prague 10400, Czech Republic Institute of Animal Science, Prague 10400, Czech Republic Institute of Animal Science, Prague 10400, Czech Republic Institute of Animal Science, Prague 10400, Czech Republic Faculty of Agrobiology Food and Natural Resources, Czech University of Life Sciences Prague, Prague 16500, Czech RepublicObjective The use of genetic markers can help to enhance reproduction in cattle, which is a very important trait for profitability in dairy production systems. This study evaluated the association between genotypes of leptin (LEP), toll-like receptor 4 (TLR4), and chemokine receptor of interleukin 8 C-X-C motif (CXCR1) genes and fertility traits in Czech Fleckvieh cattle. Methods Phenotypic data from 786 Czech Fleckvieh cows raised on 5 farms in the Czech Republic were used, along with information from the 1st three parities. To determine genotype, the polymerase chain reaction– restriction fragment length polymorphism method was used. Results Except for LEP g.-963C>T, all studied genotype frequencies of single nucleotide polymorphisms (SNPs) were distributed according to the Hardy-Weinberg equilibrium. Two LEP SNPs (g.-963C>T and c.357C>T) were associated with the age at the 1st calving, days open (DO), pregnancy rate after 1st service (PR), and calving interval (CLI). In LEP g.-963C>T the TT genotype heifers firstly calved 24 days earlier than CC genotype and the CT genotype cow showed a tendency for shorter DO and higher PR. In LEP c.357C>T we observed longer CLI and DO period in TT cows. In general, we can propose the TT genotype of g.-963C>T as favorable and the TT genotype of c.357C>T as unfavorable for a cow’s fertility. Heterozygotes in TLR4 c.-226C>G were significantly associated with shorter CLI, and presented a nonsignificant tendency to be associated with higher PR. In CXCR1 c.777 C>G, we did not observe any relationship of this SNP with reproduction. Conclusion Overall, the results showed that LEP could be an effective marker for improving reproduction in Czech Fleckvieh cattle. This study also provides novel insights into the relationship between TLR4 and CXCR1 SNPs and reproduction in dual-purpose cattle.http://www.ajas.info/upload/pdf/ajas-17-0900.pdfPolymorphismReproductionLeptinToll-like Receptor 4Chemokine Receptor of Interleukin 8 C-X-C MotifCow |
| spellingShingle | Katerina Jecminkova Uwe Müller Jitka Kyselova Zuzana Sztankoova Ludmila Zavadilova Miloslava Stipkova Ivan Majzlik Association of leptin, toll-like receptor 4, and chemokine receptor of interleukin 8 C-X-C motif single nucleotide polymorphisms with fertility traits in Czech Fleckvieh cattle Asian-Australasian Journal of Animal Sciences Polymorphism Reproduction Leptin Toll-like Receptor 4 Chemokine Receptor of Interleukin 8 C-X-C Motif Cow |
| title | Association of leptin, toll-like receptor 4, and chemokine receptor of interleukin 8 C-X-C motif single nucleotide polymorphisms with fertility traits in Czech Fleckvieh cattle |
| title_full | Association of leptin, toll-like receptor 4, and chemokine receptor of interleukin 8 C-X-C motif single nucleotide polymorphisms with fertility traits in Czech Fleckvieh cattle |
| title_fullStr | Association of leptin, toll-like receptor 4, and chemokine receptor of interleukin 8 C-X-C motif single nucleotide polymorphisms with fertility traits in Czech Fleckvieh cattle |
| title_full_unstemmed | Association of leptin, toll-like receptor 4, and chemokine receptor of interleukin 8 C-X-C motif single nucleotide polymorphisms with fertility traits in Czech Fleckvieh cattle |
| title_short | Association of leptin, toll-like receptor 4, and chemokine receptor of interleukin 8 C-X-C motif single nucleotide polymorphisms with fertility traits in Czech Fleckvieh cattle |
| title_sort | association of leptin toll like receptor 4 and chemokine receptor of interleukin 8 c x c motif single nucleotide polymorphisms with fertility traits in czech fleckvieh cattle |
| topic | Polymorphism Reproduction Leptin Toll-like Receptor 4 Chemokine Receptor of Interleukin 8 C-X-C Motif Cow |
| url | http://www.ajas.info/upload/pdf/ajas-17-0900.pdf |
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