Polyploidy induction in Physalis alkekengi
Physalis alkekengi is an ornamental plant that can also be used as a medicinal plant due to its anti-inflammatory, bactericidal, antitumor and fungicidal properties. Polyploidization can be an important tool in the genetic improvement of this species. The objective this work was to obtain tetraploid...
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Uberlândia
2020-02-01
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| Series: | Bioscience Journal |
| Subjects: | |
| Online Access: | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/47729 |
| _version_ | 1831724262502694912 |
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| author | Gustavo C Santos Felipe P Cardoso Adalvan Daniel Martins Filipe A Rodrigues Moacir Pasqual Paulo Cossani José Magno Queiroz Luz Renata A L S Rezende Joyce Dória Rodrigues Soares |
| author_facet | Gustavo C Santos Felipe P Cardoso Adalvan Daniel Martins Filipe A Rodrigues Moacir Pasqual Paulo Cossani José Magno Queiroz Luz Renata A L S Rezende Joyce Dória Rodrigues Soares |
| author_sort | Gustavo C Santos |
| collection | DOAJ |
| description | Physalis alkekengi is an ornamental plant that can also be used as a medicinal plant due to its anti-inflammatory, bactericidal, antitumor and fungicidal properties. Polyploidization can be an important tool in the genetic improvement of this species. The objective this work was to obtain tetraploids in vitro and to evaluate the phytotechnical traits of P. alkekengi. For this, nodal segments of P. alkekengi var. Franchettii were inoculated into petri dishes containing 100 ml of MS medium supplemented with colchicine at concentrations 0; 0.04; 0.08; 0.12; and 0.16% and kept in the dark for 24 and 48h. After the respective treatment periods with colchicine the segments were inoculated into test tubes. The tetraploids were identified by flow cytometry and classical cytogenetics. In vitro seedlings were measured: root length, nodal segment length, leaflet number and total leaf area. In the acclimatization phase, the area of the second leaf and total leaf, petiole radius, stem length, fruit weight with calyx, without calyx, fruit diameter, number of seeds and brix of the pulp were evaluated. Chlorophyll a, chlorophyll b, total chlorophyll, total carotenoid, total chlorophyll / total carotenoid ratio and chlorophyll a / b ratio were also estimated. The treatment that most produced tetraploid seedlings was with 0.08% colchicine per 24h. No significant difference was observed in 7 (seven) variables, these being all variables of photopigments, stem diameter (steam) and brix. In general, diploid (2x) plants were better in 9 (nine) while tetraploid seedlings were better in 6 (six) of the phytotechnical variables. It was concluded that the MS medium supplemented with 0.08% colchicine for 24 h allowed P. alkekengi tetraploides to be obtained with better phytotechnical qualities. |
| first_indexed | 2024-12-21T04:18:35Z |
| format | Article |
| id | doaj.art-c272c3bba1784c75b071d9e5eb97c81e |
| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-12-21T04:18:35Z |
| publishDate | 2020-02-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-c272c3bba1784c75b071d9e5eb97c81e2022-12-21T19:16:15ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632020-02-0136310.14393/BJ-v36n3a2020-4772947729Polyploidy induction in Physalis alkekengiGustavo C Santos0Felipe P Cardoso1Adalvan Daniel Martins2Filipe A RodriguesMoacir Pasqual3Paulo Cossani4José Magno Queiroz LuzRenata A L S Rezende5Joyce Dória Rodrigues Soares6Universidade Federal de LavrasUniversidade Federal de LavrasUniversidade Federal de LavrasUniversidade Federal de LavrasUniversidade Federal de LavrasUniversidade Federal de LavrasUniversidade Federal de LavrasPhysalis alkekengi is an ornamental plant that can also be used as a medicinal plant due to its anti-inflammatory, bactericidal, antitumor and fungicidal properties. Polyploidization can be an important tool in the genetic improvement of this species. The objective this work was to obtain tetraploids in vitro and to evaluate the phytotechnical traits of P. alkekengi. For this, nodal segments of P. alkekengi var. Franchettii were inoculated into petri dishes containing 100 ml of MS medium supplemented with colchicine at concentrations 0; 0.04; 0.08; 0.12; and 0.16% and kept in the dark for 24 and 48h. After the respective treatment periods with colchicine the segments were inoculated into test tubes. The tetraploids were identified by flow cytometry and classical cytogenetics. In vitro seedlings were measured: root length, nodal segment length, leaflet number and total leaf area. In the acclimatization phase, the area of the second leaf and total leaf, petiole radius, stem length, fruit weight with calyx, without calyx, fruit diameter, number of seeds and brix of the pulp were evaluated. Chlorophyll a, chlorophyll b, total chlorophyll, total carotenoid, total chlorophyll / total carotenoid ratio and chlorophyll a / b ratio were also estimated. The treatment that most produced tetraploid seedlings was with 0.08% colchicine per 24h. No significant difference was observed in 7 (seven) variables, these being all variables of photopigments, stem diameter (steam) and brix. In general, diploid (2x) plants were better in 9 (nine) while tetraploid seedlings were better in 6 (six) of the phytotechnical variables. It was concluded that the MS medium supplemented with 0.08% colchicine for 24 h allowed P. alkekengi tetraploides to be obtained with better phytotechnical qualities.http://www.seer.ufu.br/index.php/biosciencejournal/article/view/47729anti-mitotictetraploidmyxoplasiatissue culturephysalis |
| spellingShingle | Gustavo C Santos Felipe P Cardoso Adalvan Daniel Martins Filipe A Rodrigues Moacir Pasqual Paulo Cossani José Magno Queiroz Luz Renata A L S Rezende Joyce Dória Rodrigues Soares Polyploidy induction in Physalis alkekengi Bioscience Journal anti-mitotic tetraploid myxoplasia tissue culture physalis |
| title | Polyploidy induction in Physalis alkekengi |
| title_full | Polyploidy induction in Physalis alkekengi |
| title_fullStr | Polyploidy induction in Physalis alkekengi |
| title_full_unstemmed | Polyploidy induction in Physalis alkekengi |
| title_short | Polyploidy induction in Physalis alkekengi |
| title_sort | polyploidy induction in physalis alkekengi |
| topic | anti-mitotic tetraploid myxoplasia tissue culture physalis |
| url | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/47729 |
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