Molecular interplay between linc01134 and YY1 dictates hepatocellular carcinoma progression
Abstract Background Revealing the mechanical role of long non-coding RNAs (lncRNAs) in tumorigenesis can contribute to novel therapeutic target for cancers. The regulatory role of linc01134 in hepatocellular carcinoma (HCC) has not been studied yet. Materials and methods qRT-PCR and western blot wer...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-04-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13046-020-01551-9 |
| _version_ | 1818061707407261696 |
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| author | Zhonghou Rong Zhiyi Wang Xinxing Wang Chengkun Qin Wenmao Geng |
| author_facet | Zhonghou Rong Zhiyi Wang Xinxing Wang Chengkun Qin Wenmao Geng |
| author_sort | Zhonghou Rong |
| collection | DOAJ |
| description | Abstract Background Revealing the mechanical role of long non-coding RNAs (lncRNAs) in tumorigenesis can contribute to novel therapeutic target for cancers. The regulatory role of linc01134 in hepatocellular carcinoma (HCC) has not been studied yet. Materials and methods qRT-PCR and western blot were conducted to measure relevant RNA and protein expressions. CCK-8, colony formation, EdU, flow cytometry, wound-healing, transwell assays and xenograft experiments were performed to determine the role of linc01134 in HCC. ChIP and luciferase reporter assays were performed to analyze the effects of Yin Yang-1 (YY1) on linc01134 transcription activity. Relevant mechanical experiments were performed to verify interaction between relative genes. Results YY1 enhanced linc01134 transcription by interacting with linc01134 promoter. Knockdown of linc01134 inhibited proliferation, migration and epithelial-mesenchymal transition (EMT), yet promoting apoptosis in HCC cells. Mechanically, linc01134 acted as miR-324-5p sponge and interacted with insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1) to increase the stability of YY1 mRNA expression. Up-regulated YY1 continuously stimulated linc01134 expression by enhancing linc01134 promoter activity, forming a positive feedback loop. Conclusion Linc01134/miR-324-5p/IGF2BP1/YY1 feedback loop mediates HCC progression, which possibly provide prognosis and treatment target of HCC. |
| first_indexed | 2024-12-10T13:52:35Z |
| format | Article |
| id | doaj.art-c2737337089e4c01b32a5a689ad811d3 |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-12-10T13:52:35Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-c2737337089e4c01b32a5a689ad811d32022-12-22T01:46:09ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662020-04-0139111510.1186/s13046-020-01551-9Molecular interplay between linc01134 and YY1 dictates hepatocellular carcinoma progressionZhonghou Rong0Zhiyi Wang1Xinxing Wang2Chengkun Qin3Wenmao Geng4Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong UniversityDepartment of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong UniversityDepartment of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong UniversityDepartment of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong UniversityDepartment of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong UniversityAbstract Background Revealing the mechanical role of long non-coding RNAs (lncRNAs) in tumorigenesis can contribute to novel therapeutic target for cancers. The regulatory role of linc01134 in hepatocellular carcinoma (HCC) has not been studied yet. Materials and methods qRT-PCR and western blot were conducted to measure relevant RNA and protein expressions. CCK-8, colony formation, EdU, flow cytometry, wound-healing, transwell assays and xenograft experiments were performed to determine the role of linc01134 in HCC. ChIP and luciferase reporter assays were performed to analyze the effects of Yin Yang-1 (YY1) on linc01134 transcription activity. Relevant mechanical experiments were performed to verify interaction between relative genes. Results YY1 enhanced linc01134 transcription by interacting with linc01134 promoter. Knockdown of linc01134 inhibited proliferation, migration and epithelial-mesenchymal transition (EMT), yet promoting apoptosis in HCC cells. Mechanically, linc01134 acted as miR-324-5p sponge and interacted with insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1) to increase the stability of YY1 mRNA expression. Up-regulated YY1 continuously stimulated linc01134 expression by enhancing linc01134 promoter activity, forming a positive feedback loop. Conclusion Linc01134/miR-324-5p/IGF2BP1/YY1 feedback loop mediates HCC progression, which possibly provide prognosis and treatment target of HCC.http://link.springer.com/article/10.1186/s13046-020-01551-9YY1linc01134IGF2BP1Hepatocellular carcinoma |
| spellingShingle | Zhonghou Rong Zhiyi Wang Xinxing Wang Chengkun Qin Wenmao Geng Molecular interplay between linc01134 and YY1 dictates hepatocellular carcinoma progression Journal of Experimental & Clinical Cancer Research YY1 linc01134 IGF2BP1 Hepatocellular carcinoma |
| title | Molecular interplay between linc01134 and YY1 dictates hepatocellular carcinoma progression |
| title_full | Molecular interplay between linc01134 and YY1 dictates hepatocellular carcinoma progression |
| title_fullStr | Molecular interplay between linc01134 and YY1 dictates hepatocellular carcinoma progression |
| title_full_unstemmed | Molecular interplay between linc01134 and YY1 dictates hepatocellular carcinoma progression |
| title_short | Molecular interplay between linc01134 and YY1 dictates hepatocellular carcinoma progression |
| title_sort | molecular interplay between linc01134 and yy1 dictates hepatocellular carcinoma progression |
| topic | YY1 linc01134 IGF2BP1 Hepatocellular carcinoma |
| url | http://link.springer.com/article/10.1186/s13046-020-01551-9 |
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