ANKRD22 enhances breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expression
Breast cancer is one of the most prevalent malignancies in women worldwide. Although great advancements have been achieved in the diagnosis and treatment of breast cancer, the prognosis of patients with breast cancer is still poor due to distal recurrence and metastasis after surgery. This study ai...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2021-06-01
|
| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/4701 |
| _version_ | 1797261320249147392 |
|---|---|
| author | Yange Wu Hongxia Liu Yufeng Gong Bo Zhang Wenxiu Chen |
| author_facet | Yange Wu Hongxia Liu Yufeng Gong Bo Zhang Wenxiu Chen |
| author_sort | Yange Wu |
| collection | DOAJ |
| description |
Breast cancer is one of the most prevalent malignancies in women worldwide. Although great advancements have been achieved in the diagnosis and treatment of breast cancer, the prognosis of patients with breast cancer is still poor due to distal recurrence and metastasis after surgery. This study aimed to assess the role of ankyrin repeat domain 22 (ANKRD22) in the progression of breast cancer and investigate the molecular mechanism. Using immunohistochemistry, we demonstrated that the expression level of ANKRD22 in human breast cancer tissues was significantly higher than that in normal breast tissues. ANKRD22 knockdown inhibited the proliferation, invasion, and epithelial-mesenchymal transition (EMT) of breast cancer cells, as confirmed by BrdU, colony formation, transwell, and immunoblot assays. Immunoblot assays further indicated that ANKRD22 regulated the expression of nucleolar and spindle-associated protein 1 (NuSAP1) and then caused the activation of Wnt/β-catenin signaling pathway. Moreover, overexpression of NUSAP1 reversed the inhibitory effects of ANKRD22 knockdown on the proliferation, invasion, and EMT of breast cancer cells. In summary, this study demonstrated that ANKRD22 enhanced breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expression, which might shed light on new therapeutic approaches for breast cancer.
|
| first_indexed | 2024-04-24T23:39:20Z |
| format | Article |
| id | doaj.art-c2746fd789a849f591dd881569542633 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:39:20Z |
| publishDate | 2021-06-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-c2746fd789a849f591dd8815695426332024-03-15T13:44:22ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2021-06-0121310.17305/bjbms.2020.4701ANKRD22 enhances breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expressionYange Wu0https://orcid.org/0000-0002-5505-5514Hongxia Liu1https://orcid.org/0000-0002-0169-0168Yufeng Gong2https://orcid.org/0000-0002-2865-7229Bo Zhang3Wenxiu Chen4https://orcid.org/0000-0002-5407-1328Department of Pathology, Pingshan District People’s Hospital of Shenzhen, Pingshan General Hospital of Southern Medical University, Shenzhen, ChinaDepartment of Pathology, Pingshan District People’s Hospital of Shenzhen, Pingshan General Hospital of Southern Medical University, Shenzhen, ChinaDepartment of Pathology, Pingshan District People’s Hospital of Shenzhen, Pingshan General Hospital of Southern Medical University, Shenzhen, ChinaDepartment of Pathology, Pingshan District People’s Hospital of Shenzhen, Pingshan General Hospital of Southern Medical University, Shenzhen, ChinaDepartment of Pathology, Pingshan District People’s Hospital of Shenzhen, Pingshan General Hospital of Southern Medical University, Shenzhen, China Breast cancer is one of the most prevalent malignancies in women worldwide. Although great advancements have been achieved in the diagnosis and treatment of breast cancer, the prognosis of patients with breast cancer is still poor due to distal recurrence and metastasis after surgery. This study aimed to assess the role of ankyrin repeat domain 22 (ANKRD22) in the progression of breast cancer and investigate the molecular mechanism. Using immunohistochemistry, we demonstrated that the expression level of ANKRD22 in human breast cancer tissues was significantly higher than that in normal breast tissues. ANKRD22 knockdown inhibited the proliferation, invasion, and epithelial-mesenchymal transition (EMT) of breast cancer cells, as confirmed by BrdU, colony formation, transwell, and immunoblot assays. Immunoblot assays further indicated that ANKRD22 regulated the expression of nucleolar and spindle-associated protein 1 (NuSAP1) and then caused the activation of Wnt/β-catenin signaling pathway. Moreover, overexpression of NUSAP1 reversed the inhibitory effects of ANKRD22 knockdown on the proliferation, invasion, and EMT of breast cancer cells. In summary, this study demonstrated that ANKRD22 enhanced breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expression, which might shed light on new therapeutic approaches for breast cancer. https://www.bjbms.org/ojs/index.php/bjbms/article/view/4701Breast cancerMDA-MB-415ANKRD22NuSAP1Wnt/β-catenin pathway |
| spellingShingle | Yange Wu Hongxia Liu Yufeng Gong Bo Zhang Wenxiu Chen ANKRD22 enhances breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expression Biomolecules & Biomedicine Breast cancer MDA-MB-415 ANKRD22 NuSAP1 Wnt/β-catenin pathway |
| title | ANKRD22 enhances breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expression |
| title_full | ANKRD22 enhances breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expression |
| title_fullStr | ANKRD22 enhances breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expression |
| title_full_unstemmed | ANKRD22 enhances breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expression |
| title_short | ANKRD22 enhances breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expression |
| title_sort | ankrd22 enhances breast cancer cell malignancy by activating the wnt β catenin pathway via modulating nusap1 expression |
| topic | Breast cancer MDA-MB-415 ANKRD22 NuSAP1 Wnt/β-catenin pathway |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/4701 |
| work_keys_str_mv | AT yangewu ankrd22enhancesbreastcancercellmalignancybyactivatingthewntbcateninpathwayviamodulatingnusap1expression AT hongxialiu ankrd22enhancesbreastcancercellmalignancybyactivatingthewntbcateninpathwayviamodulatingnusap1expression AT yufenggong ankrd22enhancesbreastcancercellmalignancybyactivatingthewntbcateninpathwayviamodulatingnusap1expression AT bozhang ankrd22enhancesbreastcancercellmalignancybyactivatingthewntbcateninpathwayviamodulatingnusap1expression AT wenxiuchen ankrd22enhancesbreastcancercellmalignancybyactivatingthewntbcateninpathwayviamodulatingnusap1expression |