DICER1 and PRKRA in Colon Adenocarcinoma
Differential microRNA expression in colon adenocarcinoma (CA) was previously reported. MicroRNA biogenesis and function requires a set of proteins designated as the microRNA machinery, which includes DICER1 and PRKRA. Loss of heterozygosity at 14q32.13 DICER1 locus was detected in up to 60% of CA ca...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2008-01-01
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| Series: | Biomarker Insights |
| Online Access: | https://doi.org/10.4137/BMI.S600 |
| _version_ | 1818553645630750720 |
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| author | S. Chiosea M. Acquafondata J. Luo SF. Kuan RR. Seethala |
| author_facet | S. Chiosea M. Acquafondata J. Luo SF. Kuan RR. Seethala |
| author_sort | S. Chiosea |
| collection | DOAJ |
| description | Differential microRNA expression in colon adenocarcinoma (CA) was previously reported. MicroRNA biogenesis and function requires a set of proteins designated as the microRNA machinery, which includes DICER1 and PRKRA. Loss of heterozygosity at 14q32.13 DICER1 locus was detected in up to 60% of CA cases. The in silico gene array analysis of CA showed down-regulation of DICER1 and an up-regulation of PRKRA . Immunohistochemically, DICER1 expression was abnormal in 65% of CA (95 of 147 cases). PRKRA was deregulated in 70% of CA (32 of 46 cases). Expression of DICER1 and PRKRA was correlated with clinicopathologic features of CA. DICER1 up-regulation was seen more commonly in women. Only 10 of 46 cases immunostained for both DICER1 and PRKRA showed normal levels of both DICER1 and PRKRA. Microsatellite status of 32 cases was determined. Microsatellite instable cases showed DICER1 up-regulation more commonly when compared to microsatellite stable cases; however, this trend was not statistically significant. Abnormal DICER1 and/or PRKRA expression might explain the observed changes in microRNA profile. The status of the endogenous DICER1 and PRKRA in CA may help to predict the response to future RNA interference-based therapy. |
| first_indexed | 2024-12-12T09:28:32Z |
| format | Article |
| id | doaj.art-c278b44b054245dcb7b36458c9140d7d |
| institution | Directory Open Access Journal |
| issn | 1177-2719 |
| language | English |
| last_indexed | 2024-12-12T09:28:32Z |
| publishDate | 2008-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Biomarker Insights |
| spelling | doaj.art-c278b44b054245dcb7b36458c9140d7d2022-12-22T00:28:56ZengSAGE PublishingBiomarker Insights1177-27192008-01-01310.4137/BMI.S600DICER1 and PRKRA in Colon AdenocarcinomaS. Chiosea0M. Acquafondata1J. Luo2SF. Kuan3RR. Seethala4Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, U.S.A.Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, U.S.A.Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, U.S.A.Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, U.S.A.Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, U.S.A.Differential microRNA expression in colon adenocarcinoma (CA) was previously reported. MicroRNA biogenesis and function requires a set of proteins designated as the microRNA machinery, which includes DICER1 and PRKRA. Loss of heterozygosity at 14q32.13 DICER1 locus was detected in up to 60% of CA cases. The in silico gene array analysis of CA showed down-regulation of DICER1 and an up-regulation of PRKRA . Immunohistochemically, DICER1 expression was abnormal in 65% of CA (95 of 147 cases). PRKRA was deregulated in 70% of CA (32 of 46 cases). Expression of DICER1 and PRKRA was correlated with clinicopathologic features of CA. DICER1 up-regulation was seen more commonly in women. Only 10 of 46 cases immunostained for both DICER1 and PRKRA showed normal levels of both DICER1 and PRKRA. Microsatellite status of 32 cases was determined. Microsatellite instable cases showed DICER1 up-regulation more commonly when compared to microsatellite stable cases; however, this trend was not statistically significant. Abnormal DICER1 and/or PRKRA expression might explain the observed changes in microRNA profile. The status of the endogenous DICER1 and PRKRA in CA may help to predict the response to future RNA interference-based therapy.https://doi.org/10.4137/BMI.S600 |
| spellingShingle | S. Chiosea M. Acquafondata J. Luo SF. Kuan RR. Seethala DICER1 and PRKRA in Colon Adenocarcinoma Biomarker Insights |
| title | DICER1 and PRKRA in Colon Adenocarcinoma |
| title_full | DICER1 and PRKRA in Colon Adenocarcinoma |
| title_fullStr | DICER1 and PRKRA in Colon Adenocarcinoma |
| title_full_unstemmed | DICER1 and PRKRA in Colon Adenocarcinoma |
| title_short | DICER1 and PRKRA in Colon Adenocarcinoma |
| title_sort | dicer1 and prkra in colon adenocarcinoma |
| url | https://doi.org/10.4137/BMI.S600 |
| work_keys_str_mv | AT schiosea dicer1andprkraincolonadenocarcinoma AT macquafondata dicer1andprkraincolonadenocarcinoma AT jluo dicer1andprkraincolonadenocarcinoma AT sfkuan dicer1andprkraincolonadenocarcinoma AT rrseethala dicer1andprkraincolonadenocarcinoma |