Mitochondria Load Degree in Hepatocytes of the Classical Hepatic Lobules in Chinchilla (Chinchilla lanigera)
Hepatocytes represent the majority of the liver cell population and are arranged in the form of cords placed in intimate contact with the sinusoidal capillaries. The functional complexity corroborated with the intensity of the activity of hepatocytes requires large amounts of energy. The organelles...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AcademicPres
2021-05-01
|
| Series: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine |
| Subjects: | |
| Online Access: | http://journals.usamvcluj.ro/index.php/veterinary/article/view/14136 |
| _version_ | 1818726388940668928 |
|---|---|
| author | Ioan Florin GHIURCO Aurel DAMIAN Vasile Florin RUS Cristian MARTONOS Maria Cătălina MATEI Victoria BUZA Laura Cristina ȘTEFĂNUȚ Călin LAȚIU Ion VLASIUC Sanda Maria ANDREI Viorel MICLĂUȘ Adrian Florin GAL |
| author_facet | Ioan Florin GHIURCO Aurel DAMIAN Vasile Florin RUS Cristian MARTONOS Maria Cătălina MATEI Victoria BUZA Laura Cristina ȘTEFĂNUȚ Călin LAȚIU Ion VLASIUC Sanda Maria ANDREI Viorel MICLĂUȘ Adrian Florin GAL |
| author_sort | Ioan Florin GHIURCO |
| collection | DOAJ |
| description | Hepatocytes represent the majority of the liver cell population and are arranged in the form of cords placed in intimate contact with the sinusoidal capillaries. The functional complexity corroborated with the intensity of the activity of hepatocytes requires large amounts of energy. The organelles involved in the production of chemical energy used in the activity of hepatocytes are the mitochondria. The purpose of this study was to verify the mitochondrial load of hepatocytes in all areas of the classical hepatic lobules, in order to indirectly assess the intensity of hepatocyte activity in each area. Materials and Methods Five fresh corpses of chinchilla (Chinchilla lanigera) from an independent breeder from Bistrița-Năsăud county were used. Liver fragments were harvested and fixated in Kolster’s solution for 24 hours, stained with Heidenhain ferric hematoxylin, and assessed using Olympus BX41 microscope. Fixation with Kolster's solution and the staining with Heidenhain's iron hematoxylin clearly shows the hepatocytic mitochondria in shades from gray to black. The liver lobules displayed an uneven distribution of mitochondria depending on the area. In zone 1 of the classical hepatic lobule, the degree of loading of hepatocytes with mitochondria is larger than in zone 2 and much larger than in zone 3. Morphological features of the hepatocytes, including the number and distribution of mitochondria in the hepatic lobules, should improve the understanding of the physiology and pathology of the liver. |
| first_indexed | 2024-12-17T21:57:25Z |
| format | Article |
| id | doaj.art-c278d8f9155c4c508e1c76c58f85e73b |
| institution | Directory Open Access Journal |
| issn | 1843-5270 1843-5378 |
| language | English |
| last_indexed | 2024-12-17T21:57:25Z |
| publishDate | 2021-05-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine |
| spelling | doaj.art-c278d8f9155c4c508e1c76c58f85e73b2022-12-21T21:31:04ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine1843-52701843-53782021-05-01781768110.15835/buasvmcn-m:2021.000411536Mitochondria Load Degree in Hepatocytes of the Classical Hepatic Lobules in Chinchilla (Chinchilla lanigera)Ioan Florin GHIURCO0Aurel DAMIAN1Vasile Florin RUS2Cristian MARTONOS3Maria Cătălina MATEI4Victoria BUZA5Laura Cristina ȘTEFĂNUȚ6Călin LAȚIU7Ion VLASIUC8Sanda Maria ANDREI9Viorel MICLĂUȘ10Adrian Florin GAL11Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaFaculty of Animal Sciences, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaUniversity of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur St., 400372 Cluj-NapocaFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur Street no 3-5, Cluj-Napoca, 400372University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur Street no 3-5, Cluj-Napoca, 400372Hepatocytes represent the majority of the liver cell population and are arranged in the form of cords placed in intimate contact with the sinusoidal capillaries. The functional complexity corroborated with the intensity of the activity of hepatocytes requires large amounts of energy. The organelles involved in the production of chemical energy used in the activity of hepatocytes are the mitochondria. The purpose of this study was to verify the mitochondrial load of hepatocytes in all areas of the classical hepatic lobules, in order to indirectly assess the intensity of hepatocyte activity in each area. Materials and Methods Five fresh corpses of chinchilla (Chinchilla lanigera) from an independent breeder from Bistrița-Năsăud county were used. Liver fragments were harvested and fixated in Kolster’s solution for 24 hours, stained with Heidenhain ferric hematoxylin, and assessed using Olympus BX41 microscope. Fixation with Kolster's solution and the staining with Heidenhain's iron hematoxylin clearly shows the hepatocytic mitochondria in shades from gray to black. The liver lobules displayed an uneven distribution of mitochondria depending on the area. In zone 1 of the classical hepatic lobule, the degree of loading of hepatocytes with mitochondria is larger than in zone 2 and much larger than in zone 3. Morphological features of the hepatocytes, including the number and distribution of mitochondria in the hepatic lobules, should improve the understanding of the physiology and pathology of the liver.http://journals.usamvcluj.ro/index.php/veterinary/article/view/14136atpchinchilla lanigeraliverlobulemitochondria. |
| spellingShingle | Ioan Florin GHIURCO Aurel DAMIAN Vasile Florin RUS Cristian MARTONOS Maria Cătălina MATEI Victoria BUZA Laura Cristina ȘTEFĂNUȚ Călin LAȚIU Ion VLASIUC Sanda Maria ANDREI Viorel MICLĂUȘ Adrian Florin GAL Mitochondria Load Degree in Hepatocytes of the Classical Hepatic Lobules in Chinchilla (Chinchilla lanigera) Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine atp chinchilla lanigera liver lobule mitochondria. |
| title | Mitochondria Load Degree in Hepatocytes of the Classical Hepatic Lobules in Chinchilla (Chinchilla lanigera) |
| title_full | Mitochondria Load Degree in Hepatocytes of the Classical Hepatic Lobules in Chinchilla (Chinchilla lanigera) |
| title_fullStr | Mitochondria Load Degree in Hepatocytes of the Classical Hepatic Lobules in Chinchilla (Chinchilla lanigera) |
| title_full_unstemmed | Mitochondria Load Degree in Hepatocytes of the Classical Hepatic Lobules in Chinchilla (Chinchilla lanigera) |
| title_short | Mitochondria Load Degree in Hepatocytes of the Classical Hepatic Lobules in Chinchilla (Chinchilla lanigera) |
| title_sort | mitochondria load degree in hepatocytes of the classical hepatic lobules in chinchilla chinchilla lanigera |
| topic | atp chinchilla lanigera liver lobule mitochondria. |
| url | http://journals.usamvcluj.ro/index.php/veterinary/article/view/14136 |
| work_keys_str_mv | AT ioanfloringhiurco mitochondrialoaddegreeinhepatocytesoftheclassicalhepaticlobulesinchinchillachinchillalanigera AT aureldamian mitochondrialoaddegreeinhepatocytesoftheclassicalhepaticlobulesinchinchillachinchillalanigera AT vasileflorinrus mitochondrialoaddegreeinhepatocytesoftheclassicalhepaticlobulesinchinchillachinchillalanigera AT cristianmartonos mitochondrialoaddegreeinhepatocytesoftheclassicalhepaticlobulesinchinchillachinchillalanigera AT mariacatalinamatei mitochondrialoaddegreeinhepatocytesoftheclassicalhepaticlobulesinchinchillachinchillalanigera AT victoriabuza mitochondrialoaddegreeinhepatocytesoftheclassicalhepaticlobulesinchinchillachinchillalanigera AT lauracristinastefanut mitochondrialoaddegreeinhepatocytesoftheclassicalhepaticlobulesinchinchillachinchillalanigera AT calinlatiu mitochondrialoaddegreeinhepatocytesoftheclassicalhepaticlobulesinchinchillachinchillalanigera AT ionvlasiuc mitochondrialoaddegreeinhepatocytesoftheclassicalhepaticlobulesinchinchillachinchillalanigera AT sandamariaandrei mitochondrialoaddegreeinhepatocytesoftheclassicalhepaticlobulesinchinchillachinchillalanigera AT viorelmiclaus mitochondrialoaddegreeinhepatocytesoftheclassicalhepaticlobulesinchinchillachinchillalanigera AT adrianfloringal mitochondrialoaddegreeinhepatocytesoftheclassicalhepaticlobulesinchinchillachinchillalanigera |