Protein signatures linking history of miscarriages and metabolic syndrome: a proteomic study among North Indian women
Background Metabolic syndrome (MeS), a constellation of metabolic adversities, and history of miscarriage make women at a higher risk for cardiovascular diseases (CVDs). However, molecular evidence indicating a link between the two phenotypes (history of miscarriage and MeS) among women would offer...
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PeerJ Inc.
2019-02-01
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| Loạt: | PeerJ |
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| Truy cập trực tuyến: | https://peerj.com/articles/6321.pdf |
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| author | Saurabh Sharma Suniti Yadav Ketaki Chandiok Radhey Shyam Sharma Vandana Mishra Kallur Nava Saraswathy |
| author_facet | Saurabh Sharma Suniti Yadav Ketaki Chandiok Radhey Shyam Sharma Vandana Mishra Kallur Nava Saraswathy |
| author_sort | Saurabh Sharma |
| collection | DOAJ |
| description | Background Metabolic syndrome (MeS), a constellation of metabolic adversities, and history of miscarriage make women at a higher risk for cardiovascular diseases (CVDs). However, molecular evidence indicating a link between the two phenotypes (history of miscarriage and MeS) among women would offer an opportunity to predict the risk factor for CVDs at an early stage. Thus, the present retrospective study attempts to identify the proteins signatures (if any) to understand the connection between the history of miscarriage and MeS. Methods Age-matched 80 pre-menopausal women who were not on any medical intervention or drugs were recruited from a Mendelian population of the same gene pool. Recruited women were classified into four groups—(a) Group A—absolute cases with history of miscarriage and MeS, (b) Group B—absolute controls without any history of miscarriage and MeS, (c) Group C—cases with MeS but lack any history of miscarriage, (d) Group D—cases with history of miscarriage but lack MeS. Differentially expressed proteins in plasma samples of women from four groups were identified using 2-D gel electrophoresis and mass spectrometry. Results Three case groups (A, C, and D) showed 18 differentially expressed proteins. Nearly 60% of proteins (11/18) were commonly dysregulated in Group C (only with MeS) and Group D (only with miscarriage history). Nearly 40% of proteins (7/18) were commonly dysregulated in the three case groups (Groups A, C, and D), indicating a shared pathophysiology. Four proteins were exclusive but shared by case groups C and D indicating the independent routes for CVDs through MeS or miscarriages. In absolute cases, transthyretin (TTR) showed exclusive upregulation, which was further validated by Western blotting and ELISA. Networking analyses showed the strong association of TTR with haptoglobin, transferrin and ApoA1 hinting toward a cross-talk among these proteins which could be a cause or an effect of TTR upregulation. Conclusion The study provides evidence for molecular link between the history of miscarriage and MeS through a putative role of TTR. However, longitudinal follow-up studies with larger sample size would further help to demonstrate the significance of TTR and other targeted proteins in risk stratification and the onset of CVDs. |
| first_indexed | 2024-03-09T06:45:40Z |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:45:40Z |
| publishDate | 2019-02-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-c27a8624e3a74f5aba758e1621a266db2023-12-03T10:36:12ZengPeerJ Inc.PeerJ2167-83592019-02-017e632110.7717/peerj.6321Protein signatures linking history of miscarriages and metabolic syndrome: a proteomic study among North Indian womenSaurabh Sharma0Suniti Yadav1Ketaki Chandiok2Radhey Shyam Sharma3Vandana Mishra4Kallur Nava Saraswathy5Bioresources & Environmental Biotechnology Laboratory, Department of Environmental Studies, University of Delhi, Delhi, IndiaMolecular Anthropology Laboratory, Department of Anthropology, University of Delhi, Delhi, IndiaMolecular Anthropology Laboratory, Department of Anthropology, University of Delhi, Delhi, IndiaBioresources & Environmental Biotechnology Laboratory, Department of Environmental Studies, University of Delhi, Delhi, IndiaBioresources & Environmental Biotechnology Laboratory, Department of Environmental Studies, University of Delhi, Delhi, IndiaMolecular Anthropology Laboratory, Department of Anthropology, University of Delhi, Delhi, IndiaBackground Metabolic syndrome (MeS), a constellation of metabolic adversities, and history of miscarriage make women at a higher risk for cardiovascular diseases (CVDs). However, molecular evidence indicating a link between the two phenotypes (history of miscarriage and MeS) among women would offer an opportunity to predict the risk factor for CVDs at an early stage. Thus, the present retrospective study attempts to identify the proteins signatures (if any) to understand the connection between the history of miscarriage and MeS. Methods Age-matched 80 pre-menopausal women who were not on any medical intervention or drugs were recruited from a Mendelian population of the same gene pool. Recruited women were classified into four groups—(a) Group A—absolute cases with history of miscarriage and MeS, (b) Group B—absolute controls without any history of miscarriage and MeS, (c) Group C—cases with MeS but lack any history of miscarriage, (d) Group D—cases with history of miscarriage but lack MeS. Differentially expressed proteins in plasma samples of women from four groups were identified using 2-D gel electrophoresis and mass spectrometry. Results Three case groups (A, C, and D) showed 18 differentially expressed proteins. Nearly 60% of proteins (11/18) were commonly dysregulated in Group C (only with MeS) and Group D (only with miscarriage history). Nearly 40% of proteins (7/18) were commonly dysregulated in the three case groups (Groups A, C, and D), indicating a shared pathophysiology. Four proteins were exclusive but shared by case groups C and D indicating the independent routes for CVDs through MeS or miscarriages. In absolute cases, transthyretin (TTR) showed exclusive upregulation, which was further validated by Western blotting and ELISA. Networking analyses showed the strong association of TTR with haptoglobin, transferrin and ApoA1 hinting toward a cross-talk among these proteins which could be a cause or an effect of TTR upregulation. Conclusion The study provides evidence for molecular link between the history of miscarriage and MeS through a putative role of TTR. However, longitudinal follow-up studies with larger sample size would further help to demonstrate the significance of TTR and other targeted proteins in risk stratification and the onset of CVDs.https://peerj.com/articles/6321.pdfMetabolic syndromeProteomicsHistory of miscarriageAdverse pregnancy outcomeCardiovascular disease |
| spellingShingle | Saurabh Sharma Suniti Yadav Ketaki Chandiok Radhey Shyam Sharma Vandana Mishra Kallur Nava Saraswathy Protein signatures linking history of miscarriages and metabolic syndrome: a proteomic study among North Indian women PeerJ Metabolic syndrome Proteomics History of miscarriage Adverse pregnancy outcome Cardiovascular disease |
| title | Protein signatures linking history of miscarriages and metabolic syndrome: a proteomic study among North Indian women |
| title_full | Protein signatures linking history of miscarriages and metabolic syndrome: a proteomic study among North Indian women |
| title_fullStr | Protein signatures linking history of miscarriages and metabolic syndrome: a proteomic study among North Indian women |
| title_full_unstemmed | Protein signatures linking history of miscarriages and metabolic syndrome: a proteomic study among North Indian women |
| title_short | Protein signatures linking history of miscarriages and metabolic syndrome: a proteomic study among North Indian women |
| title_sort | protein signatures linking history of miscarriages and metabolic syndrome a proteomic study among north indian women |
| topic | Metabolic syndrome Proteomics History of miscarriage Adverse pregnancy outcome Cardiovascular disease |
| url | https://peerj.com/articles/6321.pdf |
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