Association of FCγRIIA (CD32) polymorphism with susceptibility to brucellosis
Background: Brucellosis is the major bacterial zoonoses of global importance caused by Brucella spps. FCγRIIA receptor plays a central role in phagocytosis of IgG2-opsonized bacteria. FCγRIIA exhibits allelic polymorphisms with different capacities for binding IgG2 and phagocytosis. Cells expressing...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences and Health Services
2014-08-01
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| Series: | Research in Molecular Medicine |
| Subjects: | |
| Online Access: | http://rmm.mazums.ac.ir/browse.php?a_code=A-10-25-3&slc_lang=en&sid=1 |
| _version_ | 1818278251160666112 |
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| author | Zahra Hosseini khah Zohreh Bahadori Alireza Rafiei Mehrdad Hajilooi |
| author_facet | Zahra Hosseini khah Zohreh Bahadori Alireza Rafiei Mehrdad Hajilooi |
| author_sort | Zahra Hosseini khah |
| collection | DOAJ |
| description | Background: Brucellosis is the major bacterial zoonoses of global importance caused by Brucella spps. FCγRIIA receptor plays a central role in phagocytosis of IgG2-opsonized bacteria. FCγRIIA exhibits allelic polymorphisms with different capacities for binding IgG2 and phagocytosis. Cells expressing Fc γ RIIa-H131, bind more efficiently to complexes of IgG2 than those expressing the Fc γ RII A -R131 variant. The purpose of this study was to evaluate the association of FCγRIIA polymorphisms with susceptibility to or severity of brucellosis. Materials and Methods: In this study, we evaluated FCγRIIA polymorphisms (R/R131, R/H131, H/H131) in 67 patients with brucellosis and 67 age, sex and geographical matched healthy volunteers. FCγRIIA genotyping was performed by using a sequence-specific primer polymerase chain reaction (SSP-PCR). Results: The comparison of the FCγRIIA genotypes distribution in patients with brucellosis and controls showed a higher frequency in FCγRIIA-R/R131 homozygosity in patients than controls (47.8% vs. 28.4%). Logistic regression analysis showed that there is a significant correlation between R/R131 genotype and brucellosis (OR=2.3, 95%CI=1.3-4.2, P=0.04). Although the frequency of the FCγRIIA-R/R131 was higher in patients with chronic brucellosis compared with acute brucellosis, we did not find any statistically significant differences (53.8% vs. 46.3%, P=0.65). Conclusion: The result of this study showed that the homozygous genotype of FCγRIIA-R/R131 in patients with brucellosis may be associated with susceptibility to brucellosis as a genetic risk factor. |
| first_indexed | 2024-12-12T23:14:28Z |
| format | Article |
| id | doaj.art-c27b05d5cf834c5aa4a8d41e2486c295 |
| institution | Directory Open Access Journal |
| issn | 2322-1348 2322-133X |
| language | English |
| last_indexed | 2024-12-12T23:14:28Z |
| publishDate | 2014-08-01 |
| publisher | Mazandaran University of Medical Sciences and Health Services |
| record_format | Article |
| series | Research in Molecular Medicine |
| spelling | doaj.art-c27b05d5cf834c5aa4a8d41e2486c2952022-12-22T00:08:31ZengMazandaran University of Medical Sciences and Health ServicesResearch in Molecular Medicine2322-13482322-133X2014-08-01231723Association of FCγRIIA (CD32) polymorphism with susceptibility to brucellosisZahra Hosseini khah0Zohreh Bahadori1Alireza Rafiei2Mehrdad Hajilooi3 1Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Mazandaran, Iran 2Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Tehran, Iran 1Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Mazandaran, Iran 3Department of Immunology, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran Background: Brucellosis is the major bacterial zoonoses of global importance caused by Brucella spps. FCγRIIA receptor plays a central role in phagocytosis of IgG2-opsonized bacteria. FCγRIIA exhibits allelic polymorphisms with different capacities for binding IgG2 and phagocytosis. Cells expressing Fc γ RIIa-H131, bind more efficiently to complexes of IgG2 than those expressing the Fc γ RII A -R131 variant. The purpose of this study was to evaluate the association of FCγRIIA polymorphisms with susceptibility to or severity of brucellosis. Materials and Methods: In this study, we evaluated FCγRIIA polymorphisms (R/R131, R/H131, H/H131) in 67 patients with brucellosis and 67 age, sex and geographical matched healthy volunteers. FCγRIIA genotyping was performed by using a sequence-specific primer polymerase chain reaction (SSP-PCR). Results: The comparison of the FCγRIIA genotypes distribution in patients with brucellosis and controls showed a higher frequency in FCγRIIA-R/R131 homozygosity in patients than controls (47.8% vs. 28.4%). Logistic regression analysis showed that there is a significant correlation between R/R131 genotype and brucellosis (OR=2.3, 95%CI=1.3-4.2, P=0.04). Although the frequency of the FCγRIIA-R/R131 was higher in patients with chronic brucellosis compared with acute brucellosis, we did not find any statistically significant differences (53.8% vs. 46.3%, P=0.65). Conclusion: The result of this study showed that the homozygous genotype of FCγRIIA-R/R131 in patients with brucellosis may be associated with susceptibility to brucellosis as a genetic risk factor.http://rmm.mazums.ac.ir/browse.php?a_code=A-10-25-3&slc_lang=en&sid=1Brucellosis FCγRIIA Polymorphism |
| spellingShingle | Zahra Hosseini khah Zohreh Bahadori Alireza Rafiei Mehrdad Hajilooi Association of FCγRIIA (CD32) polymorphism with susceptibility to brucellosis Research in Molecular Medicine Brucellosis FCγRIIA Polymorphism |
| title | Association of FCγRIIA (CD32) polymorphism with susceptibility to brucellosis |
| title_full | Association of FCγRIIA (CD32) polymorphism with susceptibility to brucellosis |
| title_fullStr | Association of FCγRIIA (CD32) polymorphism with susceptibility to brucellosis |
| title_full_unstemmed | Association of FCγRIIA (CD32) polymorphism with susceptibility to brucellosis |
| title_short | Association of FCγRIIA (CD32) polymorphism with susceptibility to brucellosis |
| title_sort | association of fcγriia cd32 polymorphism with susceptibility to brucellosis |
| topic | Brucellosis FCγRIIA Polymorphism |
| url | http://rmm.mazums.ac.ir/browse.php?a_code=A-10-25-3&slc_lang=en&sid=1 |
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