Minced Calf Lung Surfactant Extract Peripheral Blood Mononuclear Cells to Release IFN-γ and TGF-β: A Regulation Response for Lung
BACKGROUND: Inflammatory reactions in pathophysiologic conditions of lung are a critical problem in the treatment process, which in some cases lead to death, particularly in neonate. Exogenous lung surfactant has been considered as a candidate to treatment of inflammation in the lungs. OBJECTIVES: T...
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| Format: | Article |
| Language: | English |
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University of Tehran
2019-12-01
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| Series: | Iranian Journal of Veterinary Medicine |
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| Online Access: | https://ijvm.ut.ac.ir/article_73998_6b3b20804ec5f05f0ecd3244856033ec.pdf |
| _version_ | 1818896906850402304 |
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| author | Zohre Eftekhari Mohammd Reza Mokhber Dezfouli Babak Beikzadeh |
| author_facet | Zohre Eftekhari Mohammd Reza Mokhber Dezfouli Babak Beikzadeh |
| author_sort | Zohre Eftekhari |
| collection | DOAJ |
| description | BACKGROUND: Inflammatory reactions in pathophysiologic conditions of lung are a critical problem in the treatment process, which in some cases lead to death, particularly in neonate. Exogenous lung surfactant has been considered as a candidate to treatment of inflammation in the lungs. OBJECTIVES: The aim of this study is to examine the efficacy of this substance in vivo and in vitro. METHODS: Calf lung surfactant extract (CLSE) was obtained from freshly slaughtered calves’ minced isolates. For in vivo study: the New Zealand white rabbits as appropriate animal model were treated with formulated CLSE, then peripheral blood mononuclear cells (PBMC) were collected and the level and gene expression of IL-10, IL-6, IL-1β, IFN-γ and TGF-β were assessed before and after surfactant treatment for 30 days. In vitro study: four different formulated drug concentrations were added to rabbit PBMC and cytokines level and gene expression were evaluated. RESULTS: Our results indicate that IFN-γ and TGF-β increased at 24, 48 and 72 h which were statistically significant compared to baseline. While, IL-6 and IL-1β also started to decrease, IFN-γ and TGF-β increased due to surfactant therapy which reached its maximum expression after 7 days. CONCLUSIONS: This study suggested that CLSE could contribute in reducing pathology effects of pro-inflammatory cytokines by inducing regulatory response in lung which can be used as auxiliary and protective drug in respiratory diseases. |
| first_indexed | 2024-12-19T19:07:44Z |
| format | Article |
| id | doaj.art-c27b3dff9be44cdba723ab0933b0d92e |
| institution | Directory Open Access Journal |
| issn | 2251-8894 2252-0554 |
| language | English |
| last_indexed | 2024-12-19T19:07:44Z |
| publishDate | 2019-12-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Iranian Journal of Veterinary Medicine |
| spelling | doaj.art-c27b3dff9be44cdba723ab0933b0d92e2022-12-21T20:09:24ZengUniversity of TehranIranian Journal of Veterinary Medicine2251-88942252-05542019-12-0113434335210.22059/ijvm.2019.279096.100497973998Minced Calf Lung Surfactant Extract Peripheral Blood Mononuclear Cells to Release IFN-γ and TGF-β: A Regulation Response for LungZohre Eftekhari0Mohammd Reza Mokhber Dezfouli1Babak Beikzadeh2Quality Control Department, Research and Production Complex, Pasteur Institute of Iran, Tehran-IranDepartment of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran Institute of Biomedical Research, University of Tehran, Tehran, IranDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran. Academic Center for Education, Culture and Research (ACECR), Tehran Organization, Tehran, Iran.BACKGROUND: Inflammatory reactions in pathophysiologic conditions of lung are a critical problem in the treatment process, which in some cases lead to death, particularly in neonate. Exogenous lung surfactant has been considered as a candidate to treatment of inflammation in the lungs. OBJECTIVES: The aim of this study is to examine the efficacy of this substance in vivo and in vitro. METHODS: Calf lung surfactant extract (CLSE) was obtained from freshly slaughtered calves’ minced isolates. For in vivo study: the New Zealand white rabbits as appropriate animal model were treated with formulated CLSE, then peripheral blood mononuclear cells (PBMC) were collected and the level and gene expression of IL-10, IL-6, IL-1β, IFN-γ and TGF-β were assessed before and after surfactant treatment for 30 days. In vitro study: four different formulated drug concentrations were added to rabbit PBMC and cytokines level and gene expression were evaluated. RESULTS: Our results indicate that IFN-γ and TGF-β increased at 24, 48 and 72 h which were statistically significant compared to baseline. While, IL-6 and IL-1β also started to decrease, IFN-γ and TGF-β increased due to surfactant therapy which reached its maximum expression after 7 days. CONCLUSIONS: This study suggested that CLSE could contribute in reducing pathology effects of pro-inflammatory cytokines by inducing regulatory response in lung which can be used as auxiliary and protective drug in respiratory diseases.https://ijvm.ut.ac.ir/article_73998_6b3b20804ec5f05f0ecd3244856033ec.pdfcalf lung surfactants extractcytokines levelexogenous lung surfactantregulatory response |
| spellingShingle | Zohre Eftekhari Mohammd Reza Mokhber Dezfouli Babak Beikzadeh Minced Calf Lung Surfactant Extract Peripheral Blood Mononuclear Cells to Release IFN-γ and TGF-β: A Regulation Response for Lung Iranian Journal of Veterinary Medicine calf lung surfactants extract cytokines level exogenous lung surfactant regulatory response |
| title | Minced Calf Lung Surfactant Extract Peripheral Blood Mononuclear Cells to Release IFN-γ and TGF-β: A Regulation Response for Lung |
| title_full | Minced Calf Lung Surfactant Extract Peripheral Blood Mononuclear Cells to Release IFN-γ and TGF-β: A Regulation Response for Lung |
| title_fullStr | Minced Calf Lung Surfactant Extract Peripheral Blood Mononuclear Cells to Release IFN-γ and TGF-β: A Regulation Response for Lung |
| title_full_unstemmed | Minced Calf Lung Surfactant Extract Peripheral Blood Mononuclear Cells to Release IFN-γ and TGF-β: A Regulation Response for Lung |
| title_short | Minced Calf Lung Surfactant Extract Peripheral Blood Mononuclear Cells to Release IFN-γ and TGF-β: A Regulation Response for Lung |
| title_sort | minced calf lung surfactant extract peripheral blood mononuclear cells to release ifn γ and tgf β a regulation response for lung |
| topic | calf lung surfactants extract cytokines level exogenous lung surfactant regulatory response |
| url | https://ijvm.ut.ac.ir/article_73998_6b3b20804ec5f05f0ecd3244856033ec.pdf |
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