Invasive phenotype in triple negative breast cancer is inhibited by blocking SIN3A–PF1 interaction through KLF9 mediated repression of ITGA6 and ITGB1
SIN3A, a scaffold protein has regulatory functions in tumor biology. Through its Paired amphipathic helix (PAH2) domain, SIN3A interacts with PHF12 (PF1), a protein with SIN3 interaction domain (SID) that forms a complex with MRG15 and KDM5A/B. These components are often overexpressed in cancer. In...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-02-01
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| Series: | Translational Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523321003119 |
| _version_ | 1798035667660308480 |
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| author | Rama Kadamb Boris A Leibovitch Eduardo F Farias Nisha Dahiya Hemant Suryawanshi Nidhi Bansal Samuel Waxman |
| author_facet | Rama Kadamb Boris A Leibovitch Eduardo F Farias Nisha Dahiya Hemant Suryawanshi Nidhi Bansal Samuel Waxman |
| author_sort | Rama Kadamb |
| collection | DOAJ |
| description | SIN3A, a scaffold protein has regulatory functions in tumor biology. Through its Paired amphipathic helix (PAH2) domain, SIN3A interacts with PHF12 (PF1), a protein with SIN3 interaction domain (SID) that forms a complex with MRG15 and KDM5A/B. These components are often overexpressed in cancer. In the present study, we evaluated the role of SIN3A and its interacting partner PF1 in mediating inhibition of tumor growth and invasion in triple negative breast cancer (TNBC). We found profound inhibition of invasion, migration, and induction of cellular senescence by specific disruption of the PF1/SIN3A PAH2 domain interaction in TNBC cells expressing PF1-SID transcript or peptide treatment. Genome-wide transcriptomic analysis by RNA-seq revealed that PF1-SID downregulates several gene sets and pathways linked to invasion and migration. Integrin α6 (ITGA6) and integrin ß1 (ITGB1) and their downstream target proteins were downregulated in PF1-SID cells. We further determined increased presence of SIN3A and transcriptional repressor, KLF9, on promoters of ITGA6 and ITGB1 in PF1-SID cells. Knockdown of KLF9 leads to re-expression of ITGA6 and ITGB1 and restoration of the invasive phenotype, functionally linking KLF9 to this process. Overall, these data demonstrate that specific disruption of PF1/SIN3A, inhibits tumor growth, migration, and invasion. Also, PF1-SID not only inhibits tumor growth by senescence induction and reduced proliferation, but it also targets cancer stem cell gene expression and blocks mammosphere formation. Overall, these data demonstrate a mechanism whereby invasion and metastasis of TNBC can be suppressed by inhibiting SIN3A-PF1 interaction and enhancing KLF9 mediated suppression of ITGA6 and ITGB1. |
| first_indexed | 2024-04-11T21:00:29Z |
| format | Article |
| id | doaj.art-c27e9a15035544499057071060a93669 |
| institution | Directory Open Access Journal |
| issn | 1936-5233 |
| language | English |
| last_indexed | 2024-04-11T21:00:29Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj.art-c27e9a15035544499057071060a936692022-12-22T04:03:31ZengElsevierTranslational Oncology1936-52332022-02-0116101320Invasive phenotype in triple negative breast cancer is inhibited by blocking SIN3A–PF1 interaction through KLF9 mediated repression of ITGA6 and ITGB1Rama Kadamb0Boris A Leibovitch1Eduardo F Farias2Nisha Dahiya3Hemant Suryawanshi4Nidhi Bansal5Samuel Waxman6The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Corresponding authors.The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USAThe Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USAThe Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USAColumbia University Irving Medical Center, New York, NY, USAThe Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USAThe Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Corresponding authors.SIN3A, a scaffold protein has regulatory functions in tumor biology. Through its Paired amphipathic helix (PAH2) domain, SIN3A interacts with PHF12 (PF1), a protein with SIN3 interaction domain (SID) that forms a complex with MRG15 and KDM5A/B. These components are often overexpressed in cancer. In the present study, we evaluated the role of SIN3A and its interacting partner PF1 in mediating inhibition of tumor growth and invasion in triple negative breast cancer (TNBC). We found profound inhibition of invasion, migration, and induction of cellular senescence by specific disruption of the PF1/SIN3A PAH2 domain interaction in TNBC cells expressing PF1-SID transcript or peptide treatment. Genome-wide transcriptomic analysis by RNA-seq revealed that PF1-SID downregulates several gene sets and pathways linked to invasion and migration. Integrin α6 (ITGA6) and integrin ß1 (ITGB1) and their downstream target proteins were downregulated in PF1-SID cells. We further determined increased presence of SIN3A and transcriptional repressor, KLF9, on promoters of ITGA6 and ITGB1 in PF1-SID cells. Knockdown of KLF9 leads to re-expression of ITGA6 and ITGB1 and restoration of the invasive phenotype, functionally linking KLF9 to this process. Overall, these data demonstrate that specific disruption of PF1/SIN3A, inhibits tumor growth, migration, and invasion. Also, PF1-SID not only inhibits tumor growth by senescence induction and reduced proliferation, but it also targets cancer stem cell gene expression and blocks mammosphere formation. Overall, these data demonstrate a mechanism whereby invasion and metastasis of TNBC can be suppressed by inhibiting SIN3A-PF1 interaction and enhancing KLF9 mediated suppression of ITGA6 and ITGB1.http://www.sciencedirect.com/science/article/pii/S1936523321003119SIN3APF1SIDPAH2 domainKLF9ITGB1 |
| spellingShingle | Rama Kadamb Boris A Leibovitch Eduardo F Farias Nisha Dahiya Hemant Suryawanshi Nidhi Bansal Samuel Waxman Invasive phenotype in triple negative breast cancer is inhibited by blocking SIN3A–PF1 interaction through KLF9 mediated repression of ITGA6 and ITGB1 Translational Oncology SIN3A PF1 SID PAH2 domain KLF9 ITGB1 |
| title | Invasive phenotype in triple negative breast cancer is inhibited by blocking SIN3A–PF1 interaction through KLF9 mediated repression of ITGA6 and ITGB1 |
| title_full | Invasive phenotype in triple negative breast cancer is inhibited by blocking SIN3A–PF1 interaction through KLF9 mediated repression of ITGA6 and ITGB1 |
| title_fullStr | Invasive phenotype in triple negative breast cancer is inhibited by blocking SIN3A–PF1 interaction through KLF9 mediated repression of ITGA6 and ITGB1 |
| title_full_unstemmed | Invasive phenotype in triple negative breast cancer is inhibited by blocking SIN3A–PF1 interaction through KLF9 mediated repression of ITGA6 and ITGB1 |
| title_short | Invasive phenotype in triple negative breast cancer is inhibited by blocking SIN3A–PF1 interaction through KLF9 mediated repression of ITGA6 and ITGB1 |
| title_sort | invasive phenotype in triple negative breast cancer is inhibited by blocking sin3a pf1 interaction through klf9 mediated repression of itga6 and itgb1 |
| topic | SIN3A PF1 SID PAH2 domain KLF9 ITGB1 |
| url | http://www.sciencedirect.com/science/article/pii/S1936523321003119 |
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