Disruption of Adipokinetic Hormone Mediated Energy Homeostasis Has Subtle Effects on Physiology, Behavior and Lipid Status During Aging in Drosophila
The impact of disruption of adipokinetic hormone (AKH) signaling was studied during aging in Drosophila in a sexually dimorphic manner. A mutant (Akh1) producing a non-functional AKH peptide was compared with isogenized wild-type controls (w1118), and Akh-rescue line where AKH was ectopically expres...
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2018.00949/full |
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| author | Andrea Bednářová Andrea Bednářová Aleš Tomčala Michaela Mochanová Michaela Mochanová Dalibor Kodrík Dalibor Kodrík Natraj Krishnan |
| author_facet | Andrea Bednářová Andrea Bednářová Aleš Tomčala Michaela Mochanová Michaela Mochanová Dalibor Kodrík Dalibor Kodrík Natraj Krishnan |
| author_sort | Andrea Bednářová |
| collection | DOAJ |
| description | The impact of disruption of adipokinetic hormone (AKH) signaling was studied during aging in Drosophila in a sexually dimorphic manner. A mutant (Akh1) producing a non-functional AKH peptide was compared with isogenized wild-type controls (w1118), and Akh-rescue line where AKH was ectopically expressed in the mutant background (EE-Akh). Longevity, fecundity, and locomotor activity rhythms remained unaffected by lack of AKH signaling. While the strength of rhythms declined in general with age across all fly lines tested this was more so in case of Akh1 flies. Negative geotaxis was significantly impaired in Akh1 flies. Only young Akh1 flies of both sexes and old Akh1 females showed significantly higher body weight compared to age-matched iso-control flies (except in case of EE-Akh). Expression of genes involved in energy homeostasis and aging indicated that dTOR and Akt expression were elevated in Akh1 flies compared to other genotypes, whereas AMPK and dFoxO expression levels were significantly reduced. Multivariate analysis of the distribution of lipid species revealed a significant accumulation of specific diglyceride (DG) and triglyceride (TG) lipid species, irrespective of sex, attributable in part due to lack of AKH. Moreover, irrespective of lack of AKH, older flies of all genotypes accumulated TGs. Taken together, the results strongly suggest that disruption of AKH has very subtle effects on physiology, behavior and lipid status during aging. |
| first_indexed | 2024-04-12T08:57:30Z |
| format | Article |
| id | doaj.art-c27f2071cec6479984eeab469dc87dcb |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-12T08:57:30Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-c27f2071cec6479984eeab469dc87dcb2022-12-22T03:39:21ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-07-01910.3389/fphys.2018.00949393327Disruption of Adipokinetic Hormone Mediated Energy Homeostasis Has Subtle Effects on Physiology, Behavior and Lipid Status During Aging in DrosophilaAndrea Bednářová0Andrea Bednářová1Aleš Tomčala2Michaela Mochanová3Michaela Mochanová4Dalibor Kodrík5Dalibor Kodrík6Natraj Krishnan7Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, MS, United StatesBiology Centre, Institute of Entomology, Academy of Sciences, České Budějovice, CzechiaBiology Centre, Institute of Parasitology, Academy of Sciences, České Budějovice, CzechiaBiology Centre, Institute of Entomology, Academy of Sciences, České Budějovice, CzechiaFaculty of Science, University of South Bohemia, České Budějovice, CzechiaBiology Centre, Institute of Entomology, Academy of Sciences, České Budějovice, CzechiaFaculty of Science, University of South Bohemia, České Budějovice, CzechiaDepartment of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, MS, United StatesThe impact of disruption of adipokinetic hormone (AKH) signaling was studied during aging in Drosophila in a sexually dimorphic manner. A mutant (Akh1) producing a non-functional AKH peptide was compared with isogenized wild-type controls (w1118), and Akh-rescue line where AKH was ectopically expressed in the mutant background (EE-Akh). Longevity, fecundity, and locomotor activity rhythms remained unaffected by lack of AKH signaling. While the strength of rhythms declined in general with age across all fly lines tested this was more so in case of Akh1 flies. Negative geotaxis was significantly impaired in Akh1 flies. Only young Akh1 flies of both sexes and old Akh1 females showed significantly higher body weight compared to age-matched iso-control flies (except in case of EE-Akh). Expression of genes involved in energy homeostasis and aging indicated that dTOR and Akt expression were elevated in Akh1 flies compared to other genotypes, whereas AMPK and dFoxO expression levels were significantly reduced. Multivariate analysis of the distribution of lipid species revealed a significant accumulation of specific diglyceride (DG) and triglyceride (TG) lipid species, irrespective of sex, attributable in part due to lack of AKH. Moreover, irrespective of lack of AKH, older flies of all genotypes accumulated TGs. Taken together, the results strongly suggest that disruption of AKH has very subtle effects on physiology, behavior and lipid status during aging.https://www.frontiersin.org/article/10.3389/fphys.2018.00949/fulladipokinetic hormoneagingAKH signalinglipid statussenescenceenergy homeostasis |
| spellingShingle | Andrea Bednářová Andrea Bednářová Aleš Tomčala Michaela Mochanová Michaela Mochanová Dalibor Kodrík Dalibor Kodrík Natraj Krishnan Disruption of Adipokinetic Hormone Mediated Energy Homeostasis Has Subtle Effects on Physiology, Behavior and Lipid Status During Aging in Drosophila Frontiers in Physiology adipokinetic hormone aging AKH signaling lipid status senescence energy homeostasis |
| title | Disruption of Adipokinetic Hormone Mediated Energy Homeostasis Has Subtle Effects on Physiology, Behavior and Lipid Status During Aging in Drosophila |
| title_full | Disruption of Adipokinetic Hormone Mediated Energy Homeostasis Has Subtle Effects on Physiology, Behavior and Lipid Status During Aging in Drosophila |
| title_fullStr | Disruption of Adipokinetic Hormone Mediated Energy Homeostasis Has Subtle Effects on Physiology, Behavior and Lipid Status During Aging in Drosophila |
| title_full_unstemmed | Disruption of Adipokinetic Hormone Mediated Energy Homeostasis Has Subtle Effects on Physiology, Behavior and Lipid Status During Aging in Drosophila |
| title_short | Disruption of Adipokinetic Hormone Mediated Energy Homeostasis Has Subtle Effects on Physiology, Behavior and Lipid Status During Aging in Drosophila |
| title_sort | disruption of adipokinetic hormone mediated energy homeostasis has subtle effects on physiology behavior and lipid status during aging in drosophila |
| topic | adipokinetic hormone aging AKH signaling lipid status senescence energy homeostasis |
| url | https://www.frontiersin.org/article/10.3389/fphys.2018.00949/full |
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