Effects of dietary bacitracin or Bacillus subtilis on the woody breast myopathy-associated gut microbiome of Eimeria spp. challenged and unchallenged broilers
ABSTRACT: Study suggested that dysbiosis of the gut microbiota may affect the etiology of woody breast (WB). In the current study, the cecal microbiota and WB in chickens fed three different diets were investigated. A total of 504 male chicks were used in a randomized complete block design with a 3...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122002528 |
| _version_ | 1818536122351878144 |
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| author | Linan Jia Chuan-Yu Hsu Xue Zhang Xiaofei Li M. Wes Schilling E. David Peebles Aaron S. Kiess Li Zhang |
| author_facet | Linan Jia Chuan-Yu Hsu Xue Zhang Xiaofei Li M. Wes Schilling E. David Peebles Aaron S. Kiess Li Zhang |
| author_sort | Linan Jia |
| collection | DOAJ |
| description | ABSTRACT: Study suggested that dysbiosis of the gut microbiota may affect the etiology of woody breast (WB). In the current study, the cecal microbiota and WB in chickens fed three different diets were investigated. A total of 504 male chicks were used in a randomized complete block design with a 3 (Diet) × 2 (Challenge) factorial arrangement of treatments with 6 replicates per treatment, 6 treatments per block, and 14 birds per treatment. The experimental diets were a control diet (corn-soybean meal basal diet), an antibiotic diet (basal diet + 6.075 mg bacitracin/kg feed), and a probiotic diet (basal diet + 2.2 × 108 CFU Bacillus subtilis PB6/kg feed). On d 14, birds that were assigned to the challenge treatment received a 20 × live cocci vaccine. On d 41, breast muscle hardness in live birds was palpated and grouped into normal (NB) and WB phenotypes. Cecal contents were collected and their bacterial compositions were analyzed and compared. The genomic DNA of the cecal contents was extracted and the V3 and V4 regions of 16S rRNA gene were amplified and sequenced via an Illumina MiSeq platform. There were no differences (P > 0.05) in Shannon and Chao 1 indexes between the challenges, diets, and phenotypes (NB vs. WB). However, there was a difference (P = 0.001) in the beta diversity of the samples between the challenged and nonchallenged groups. Relative bacterial abundance differed (false discovery rate, FDR < 0.05) between the challenge treatments, but there were no significant differences (FDR > 0.05) among the three diets or two phenotypes. Predicted energy metabolism, nucleotide metabolism, and amino acid and coenzyme biosynthesis activities only differed (q-value < 0.05) between challenged and nonchallenged groups. The cocci challenge altered the gut microbial composition on Butyricicoccus pullicaecorum, Sporobacter termitidis, and Subdoligranulum variabile, but the dietary antibiotic and probiotic treatments did not impact gut microbial composition. No strong association was found between WB myopathy and gut microbial composition in this study. |
| first_indexed | 2024-12-11T18:33:53Z |
| format | Article |
| id | doaj.art-c281addec3b449cfa1e81c576bb2783c |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-11T18:33:53Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-c281addec3b449cfa1e81c576bb2783c2022-12-22T00:54:49ZengElsevierPoultry Science0032-57912022-08-011018101960Effects of dietary bacitracin or Bacillus subtilis on the woody breast myopathy-associated gut microbiome of Eimeria spp. challenged and unchallenged broilersLinan Jia0Chuan-Yu Hsu1Xue Zhang2Xiaofei Li3M. Wes Schilling4E. David Peebles5Aaron S. Kiess6Li Zhang7Department of Poultry Science, Mississippi State University, Mississippi State, MS, 39762, USAInstitute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, MS, 39762, USADepartment of Food Science, Nutrition and Health Promotion, Mississippi State University, Mississippi State, MS, 39762, USADepartment of Agricultural Economics, Mississippi State University, Mississippi State, MS, 39762, USADepartment of Food Science, Nutrition and Health Promotion, Mississippi State University, Mississippi State, MS, 39762, USADepartment of Poultry Science, Mississippi State University, Mississippi State, MS, 39762, USAPrestage Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695, USADepartment of Poultry Science, Mississippi State University, Mississippi State, MS, 39762, USA; Corresponding author:ABSTRACT: Study suggested that dysbiosis of the gut microbiota may affect the etiology of woody breast (WB). In the current study, the cecal microbiota and WB in chickens fed three different diets were investigated. A total of 504 male chicks were used in a randomized complete block design with a 3 (Diet) × 2 (Challenge) factorial arrangement of treatments with 6 replicates per treatment, 6 treatments per block, and 14 birds per treatment. The experimental diets were a control diet (corn-soybean meal basal diet), an antibiotic diet (basal diet + 6.075 mg bacitracin/kg feed), and a probiotic diet (basal diet + 2.2 × 108 CFU Bacillus subtilis PB6/kg feed). On d 14, birds that were assigned to the challenge treatment received a 20 × live cocci vaccine. On d 41, breast muscle hardness in live birds was palpated and grouped into normal (NB) and WB phenotypes. Cecal contents were collected and their bacterial compositions were analyzed and compared. The genomic DNA of the cecal contents was extracted and the V3 and V4 regions of 16S rRNA gene were amplified and sequenced via an Illumina MiSeq platform. There were no differences (P > 0.05) in Shannon and Chao 1 indexes between the challenges, diets, and phenotypes (NB vs. WB). However, there was a difference (P = 0.001) in the beta diversity of the samples between the challenged and nonchallenged groups. Relative bacterial abundance differed (false discovery rate, FDR < 0.05) between the challenge treatments, but there were no significant differences (FDR > 0.05) among the three diets or two phenotypes. Predicted energy metabolism, nucleotide metabolism, and amino acid and coenzyme biosynthesis activities only differed (q-value < 0.05) between challenged and nonchallenged groups. The cocci challenge altered the gut microbial composition on Butyricicoccus pullicaecorum, Sporobacter termitidis, and Subdoligranulum variabile, but the dietary antibiotic and probiotic treatments did not impact gut microbial composition. No strong association was found between WB myopathy and gut microbial composition in this study.http://www.sciencedirect.com/science/article/pii/S0032579122002528broiler chickenwoody breastgut microbiomeEimeriaprobiotics |
| spellingShingle | Linan Jia Chuan-Yu Hsu Xue Zhang Xiaofei Li M. Wes Schilling E. David Peebles Aaron S. Kiess Li Zhang Effects of dietary bacitracin or Bacillus subtilis on the woody breast myopathy-associated gut microbiome of Eimeria spp. challenged and unchallenged broilers Poultry Science broiler chicken woody breast gut microbiome Eimeria probiotics |
| title | Effects of dietary bacitracin or Bacillus subtilis on the woody breast myopathy-associated gut microbiome of Eimeria spp. challenged and unchallenged broilers |
| title_full | Effects of dietary bacitracin or Bacillus subtilis on the woody breast myopathy-associated gut microbiome of Eimeria spp. challenged and unchallenged broilers |
| title_fullStr | Effects of dietary bacitracin or Bacillus subtilis on the woody breast myopathy-associated gut microbiome of Eimeria spp. challenged and unchallenged broilers |
| title_full_unstemmed | Effects of dietary bacitracin or Bacillus subtilis on the woody breast myopathy-associated gut microbiome of Eimeria spp. challenged and unchallenged broilers |
| title_short | Effects of dietary bacitracin or Bacillus subtilis on the woody breast myopathy-associated gut microbiome of Eimeria spp. challenged and unchallenged broilers |
| title_sort | effects of dietary bacitracin or bacillus subtilis on the woody breast myopathy associated gut microbiome of eimeria spp challenged and unchallenged broilers |
| topic | broiler chicken woody breast gut microbiome Eimeria probiotics |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122002528 |
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