PKCβII-induced upregulation of PGP9.5 and VEGF in postoperative persistent pain in rats
Xiang Zhu,* Yuxi Liu,* Hongfang Huang, Yonghua Zhang, Saisai Huang, Weiwei Zhou, Xiaocui Bian, Shiren Shen, Su Cao Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, People’s Republic of China *These authors contributed equally to this work Pu...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2018-09-01
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| Series: | Journal of Pain Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/pkcbetaii-induced-upregulation-of-pgp95-and-vegf-in-postoperative-pers-peer-reviewed-article-JPR |
| _version_ | 1828301701883363328 |
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| author | Zhu X Liu Y Huang H Zhang Y Huang S Zhou W Bian X Shen S Cao S |
| author_facet | Zhu X Liu Y Huang H Zhang Y Huang S Zhou W Bian X Shen S Cao S |
| author_sort | Zhu X |
| collection | DOAJ |
| description | Xiang Zhu,* Yuxi Liu,* Hongfang Huang, Yonghua Zhang, Saisai Huang, Weiwei Zhou, Xiaocui Bian, Shiren Shen, Su Cao Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, People’s Republic of China *These authors contributed equally to this work Purpose: Postoperative pain is a common clinical problem. In this study, we aimed to investigate the role of protein kinase C bII (PKCβII) in the progression of postoperative pain following skin/muscle incision and retraction (SMIR) surgery. Materials and methods: SMIR postoperative pain model was established in rats, akin to a clinical procedure. The expression level and location of p-PKCβII were observed in dorsal root ganglion (DRG) or spinal cord from SMIR-operated rats by Western blotting and immunofluorescence. In addition, the effects of PKCβII on the expression of protein gene product 9.5 (PGP9.5) or vascular endothelial growth factor (VEGF) were assessed by using pharmacological activator and inhibitor of PKCβII. Moreover, mechanical withdrawal threshold (MWT) was assessed before or after SMIR-operated rats were treated with inhibitor or activator of PKCβII. Results: The expression of PKCβII in DRG and spinal cord was significantly increased after SMIR surgery (P < 0.001, P < 0.01) and expression of PKCβII was located in the neurons of the spinal cord, and magnocellular neurons, non-peptide neurons, and peptide neurons in DRG. Besides, compared with skin/muscle incision group, retraction caused a marked increase in the expression of PKCβII and a significant decrease of MWT (P < 0.001, P < 0.05). The activator of PKCβII greatly increased the expression of PGP9.5 and VEGF (P < 0.05, P < 0.01) and enhanced MWT (P < 0.001), while inhibitor of PKCβII decreased the expression of PGP9.5 and VEGF and attenuated MWT (P < 0.05, P < 0.01, P < 0.001). Conclusion: Activation of PKCβII signaling pathways might be an important mechanism in the progression of postoperative pain. Keywords: postoperative persistent pain, neurons, PKCβII, PGP9.5, VEGF |
| first_indexed | 2024-04-13T13:26:30Z |
| format | Article |
| id | doaj.art-c2881754dc9142f1b6ab3034c89937a2 |
| institution | Directory Open Access Journal |
| issn | 1178-7090 |
| language | English |
| last_indexed | 2024-04-13T13:26:30Z |
| publishDate | 2018-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Pain Research |
| spelling | doaj.art-c2881754dc9142f1b6ab3034c89937a22022-12-22T02:45:08ZengDove Medical PressJournal of Pain Research1178-70902018-09-01Volume 112095210640949PKCβII-induced upregulation of PGP9.5 and VEGF in postoperative persistent pain in ratsZhu XLiu YHuang HZhang YHuang SZhou WBian XShen SCao SXiang Zhu,* Yuxi Liu,* Hongfang Huang, Yonghua Zhang, Saisai Huang, Weiwei Zhou, Xiaocui Bian, Shiren Shen, Su Cao Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, People’s Republic of China *These authors contributed equally to this work Purpose: Postoperative pain is a common clinical problem. In this study, we aimed to investigate the role of protein kinase C bII (PKCβII) in the progression of postoperative pain following skin/muscle incision and retraction (SMIR) surgery. Materials and methods: SMIR postoperative pain model was established in rats, akin to a clinical procedure. The expression level and location of p-PKCβII were observed in dorsal root ganglion (DRG) or spinal cord from SMIR-operated rats by Western blotting and immunofluorescence. In addition, the effects of PKCβII on the expression of protein gene product 9.5 (PGP9.5) or vascular endothelial growth factor (VEGF) were assessed by using pharmacological activator and inhibitor of PKCβII. Moreover, mechanical withdrawal threshold (MWT) was assessed before or after SMIR-operated rats were treated with inhibitor or activator of PKCβII. Results: The expression of PKCβII in DRG and spinal cord was significantly increased after SMIR surgery (P < 0.001, P < 0.01) and expression of PKCβII was located in the neurons of the spinal cord, and magnocellular neurons, non-peptide neurons, and peptide neurons in DRG. Besides, compared with skin/muscle incision group, retraction caused a marked increase in the expression of PKCβII and a significant decrease of MWT (P < 0.001, P < 0.05). The activator of PKCβII greatly increased the expression of PGP9.5 and VEGF (P < 0.05, P < 0.01) and enhanced MWT (P < 0.001), while inhibitor of PKCβII decreased the expression of PGP9.5 and VEGF and attenuated MWT (P < 0.05, P < 0.01, P < 0.001). Conclusion: Activation of PKCβII signaling pathways might be an important mechanism in the progression of postoperative pain. Keywords: postoperative persistent pain, neurons, PKCβII, PGP9.5, VEGFhttps://www.dovepress.com/pkcbetaii-induced-upregulation-of-pgp95-and-vegf-in-postoperative-pers-peer-reviewed-article-JPRpostoperative persistent painneuronsPKCβIIPGP9.5VEGF |
| spellingShingle | Zhu X Liu Y Huang H Zhang Y Huang S Zhou W Bian X Shen S Cao S PKCβII-induced upregulation of PGP9.5 and VEGF in postoperative persistent pain in rats Journal of Pain Research postoperative persistent pain neurons PKCβII PGP9.5 VEGF |
| title | PKCβII-induced upregulation of PGP9.5 and VEGF in postoperative persistent pain in rats |
| title_full | PKCβII-induced upregulation of PGP9.5 and VEGF in postoperative persistent pain in rats |
| title_fullStr | PKCβII-induced upregulation of PGP9.5 and VEGF in postoperative persistent pain in rats |
| title_full_unstemmed | PKCβII-induced upregulation of PGP9.5 and VEGF in postoperative persistent pain in rats |
| title_short | PKCβII-induced upregulation of PGP9.5 and VEGF in postoperative persistent pain in rats |
| title_sort | pkc beta ii induced upregulation of pgp9 5 and vegf in postoperative persistent pain in rats |
| topic | postoperative persistent pain neurons PKCβII PGP9.5 VEGF |
| url | https://www.dovepress.com/pkcbetaii-induced-upregulation-of-pgp95-and-vegf-in-postoperative-pers-peer-reviewed-article-JPR |
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