Wound healing activity of Tropaeolum tuberosum-based topical formulations in mice
Background and Aim: Tropaeolum tuberosum Ruiz and Pavón, a tuber native to South America, is characterized by its antioxidant, antimicrobial, and anti-inflammatory properties that contribute to wound healing. This study aimed to evaluate the healing effect of the topical T. tuberosum formulations (g...
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| Format: | Article |
| Language: | English |
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Veterinary World
2022-02-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.15/February-2022/20.pdf |
| _version_ | 1818296813776535552 |
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| author | Carmen R. Silva-Correa Greysi I. Pazo-Medina Víctor E. Villarreal-La Torre Abhel A. Calderón-Peña Cinthya L. Aspajo-Villalaz José L. Cruzado-Razco Jorge Del Rosario-Chávarri Anabel D. González-Siccha Luz M. Guerrero-Espino María V. González-Blas William A. Sagástegui-Guarniz César D. Gamarra-Sánchez Julio Hilario-Vargas |
| author_facet | Carmen R. Silva-Correa Greysi I. Pazo-Medina Víctor E. Villarreal-La Torre Abhel A. Calderón-Peña Cinthya L. Aspajo-Villalaz José L. Cruzado-Razco Jorge Del Rosario-Chávarri Anabel D. González-Siccha Luz M. Guerrero-Espino María V. González-Blas William A. Sagástegui-Guarniz César D. Gamarra-Sánchez Julio Hilario-Vargas |
| author_sort | Carmen R. Silva-Correa |
| collection | DOAJ |
| description | Background and Aim: Tropaeolum tuberosum Ruiz and Pavón, a tuber native to South America, is characterized by its antioxidant, antimicrobial, and anti-inflammatory properties that contribute to wound healing. This study aimed to evaluate the healing effect of the topical T. tuberosum formulations (gel and cream) on induced wounds in mice.
Materials and Methods: Here, an acidic ethanolic extract (1.5 N hydrochloric acid and 96% ethanol at the ratio of 15:85, v/v) was prepared with the tubers of T. tuberosum ecotype black and incorporated into topical cream and gel formulations at 1%. Thirty-twoBalb/c mice were divided into four experimental groups receiving daily topical treatments for 14 days: Group I (control; no treatment), Group II (a commercial ointment of neomycin, polymyxin B, and bacitracin), Group III (1% T. tuberosum gel), and Group IV (1% T. tuberosum cream). The wound closure in the mice was determined during the treatment; then, they were euthanized to obtain skin samples for histopathological analysis.
Results: Groups III and IV showed a higher percentage of wound closure from the 6th day of the treatment. From the 8th day, the effect was greater in Group III. The healing effect was confirmed by the histopathological changes.
Conclusion: This study concludes that the topical formulations of T. tuberosum demonstrate wound-healing activity in mice, and the most effective treatment is the 1% gel formulation. |
| first_indexed | 2024-12-13T04:09:30Z |
| format | Article |
| id | doaj.art-c28891c472364588af05feba5f0eca68 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-13T04:09:30Z |
| publishDate | 2022-02-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-c28891c472364588af05feba5f0eca682022-12-22T00:00:05ZengVeterinary WorldVeterinary World0972-89882231-09162022-02-0115239039610.14202/vetworld.2022.390-396Wound healing activity of Tropaeolum tuberosum-based topical formulations in miceCarmen R. Silva-Correa0https://orcid.org/0000-0001-9866-2739Greysi I. Pazo-Medina1https://orcid.org/0000-0002-4442-6471Víctor E. Villarreal-La Torre2https://orcid.org/0000-0003-3956-3559Abhel A. Calderón-Peña3https://orcid.org/0000-0001-7953-9874Cinthya L. Aspajo-Villalaz4https://orcid.org/0000-0002-7776-5488José L. Cruzado-Razco5https://orcid.org/0000-0001-8731-283XJorge Del Rosario-Chávarri6https://orcid.org/0000-0003-0794-1260Anabel D. González-Siccha7https://orcid.org/0000-0002-0934-1317Luz M. Guerrero-Espino8https://orcid.org/0000-0001-9331-6554María V. González-Blas9https://orcid.org/0000-0002-6168-3568William A. Sagástegui-Guarniz10https://orcid.org/0000-0002-4582-9547César D. Gamarra-Sánchez11https://orcid.org/0000-0003-4244-7460Julio Hilario-Vargas12https://orcid.org/0000-0002-8656-6843Department of Pharmacology, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Perú.Department of Pharmacology, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Perú.Department of Pharmacology, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Perú.Department of Biological Chemistry and Animal Physiology, School of Biological Science, National University of Trujillo, Trujillo, Perú.Department of Biological Chemistry and Animal Physiology, School of Biological Science, National University of Trujillo, Trujillo, Perú.Department of Pharmacology, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Perú.Department of Biological Chemistry and Animal Physiology, School of Biological Science, National University of Trujillo, Trujillo, Perú.Department of Biochemistry, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Perú.Department of Physiology, School of Medicine, National University of Trujillo, Trujillo, Perú.Department of Biochemistry, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Perú.Department of Pharmacology, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Perú.Department of Pharmacology, School of Pharmacy and Biochemistry, National University of Trujillo, Trujillo, Perú.Department of Physiology, School of Medicine, National University of Trujillo, Trujillo, Perú.Background and Aim: Tropaeolum tuberosum Ruiz and Pavón, a tuber native to South America, is characterized by its antioxidant, antimicrobial, and anti-inflammatory properties that contribute to wound healing. This study aimed to evaluate the healing effect of the topical T. tuberosum formulations (gel and cream) on induced wounds in mice. Materials and Methods: Here, an acidic ethanolic extract (1.5 N hydrochloric acid and 96% ethanol at the ratio of 15:85, v/v) was prepared with the tubers of T. tuberosum ecotype black and incorporated into topical cream and gel formulations at 1%. Thirty-twoBalb/c mice were divided into four experimental groups receiving daily topical treatments for 14 days: Group I (control; no treatment), Group II (a commercial ointment of neomycin, polymyxin B, and bacitracin), Group III (1% T. tuberosum gel), and Group IV (1% T. tuberosum cream). The wound closure in the mice was determined during the treatment; then, they were euthanized to obtain skin samples for histopathological analysis. Results: Groups III and IV showed a higher percentage of wound closure from the 6th day of the treatment. From the 8th day, the effect was greater in Group III. The healing effect was confirmed by the histopathological changes. Conclusion: This study concludes that the topical formulations of T. tuberosum demonstrate wound-healing activity in mice, and the most effective treatment is the 1% gel formulation.http://www.veterinaryworld.org/Vol.15/February-2022/20.pdfhistologyhistopathological changesskintopical formulationstropaeolum tuberosumwound healing |
| spellingShingle | Carmen R. Silva-Correa Greysi I. Pazo-Medina Víctor E. Villarreal-La Torre Abhel A. Calderón-Peña Cinthya L. Aspajo-Villalaz José L. Cruzado-Razco Jorge Del Rosario-Chávarri Anabel D. González-Siccha Luz M. Guerrero-Espino María V. González-Blas William A. Sagástegui-Guarniz César D. Gamarra-Sánchez Julio Hilario-Vargas Wound healing activity of Tropaeolum tuberosum-based topical formulations in mice Veterinary World histology histopathological changes skin topical formulations tropaeolum tuberosum wound healing |
| title | Wound healing activity of Tropaeolum tuberosum-based topical formulations in mice |
| title_full | Wound healing activity of Tropaeolum tuberosum-based topical formulations in mice |
| title_fullStr | Wound healing activity of Tropaeolum tuberosum-based topical formulations in mice |
| title_full_unstemmed | Wound healing activity of Tropaeolum tuberosum-based topical formulations in mice |
| title_short | Wound healing activity of Tropaeolum tuberosum-based topical formulations in mice |
| title_sort | wound healing activity of tropaeolum tuberosum based topical formulations in mice |
| topic | histology histopathological changes skin topical formulations tropaeolum tuberosum wound healing |
| url | http://www.veterinaryworld.org/Vol.15/February-2022/20.pdf |
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