Improving Magnetofection of Magnetic Polyethylenimine Nanoparticles into MG-63 Osteoblasts Using a Novel Uniform Magnetic Field
Abstract This study aimed to improve the magnetofection of MG-63 osteoblasts by integrating the use of a novel uniform magnetic field with low molecular weight polyethylenimine modified superparamagnetic iron oxide nanoparticles (PEI-SPIO-NPs). The excellent characteristics of PEI-SPIO-NPs such as s...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-03-01
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| Series: | Nanoscale Research Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s11671-019-2882-5 |
| _version_ | 1827887802221592576 |
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| author | Chaode Cen Jun Wu Yong Zhang Cong Luo Lina Xie Xin Zhang Xiaolan Yang Ming Li Yang Bi Tingyu Li Tongchuan He |
| author_facet | Chaode Cen Jun Wu Yong Zhang Cong Luo Lina Xie Xin Zhang Xiaolan Yang Ming Li Yang Bi Tingyu Li Tongchuan He |
| author_sort | Chaode Cen |
| collection | DOAJ |
| description | Abstract This study aimed to improve the magnetofection of MG-63 osteoblasts by integrating the use of a novel uniform magnetic field with low molecular weight polyethylenimine modified superparamagnetic iron oxide nanoparticles (PEI-SPIO-NPs). The excellent characteristics of PEI-SPIO-NPs such as size, zeta potential, the pDNA binding and protective ability were determined to be suitable for gene delivery. The novel uniform magnetic field enabled polyethylenimine-modified superparamagnetic iron oxide nanoparticles/pDNA complexes (PEI-SPIO-NPs/pDNA complexes) to rapidly and uniformly distribute on the surface of MG-63 cells, averting local transfection and decreasing disruption of the membrane caused by the centralization of positively charged PEI-SPIO-NPs, thereby increasing the effective coverage of magnetic gene carriers during transfection, and improving magnetofection efficiency. This innovative uniform magnetic field can be used to determine the optimal amount between PEI-SPIO-NPs and pDNA, as well as screen for the optimal formulation design of magnetic gene carrier under the homogenous conditions. Most importantly, the novel uniform magnetic field facilitates the transfection of PEI-SPIO-NPs/pDNA into osteoblasts, thereby providing a novel approach for the targeted delivery of therapeutic genes to osteosarcoma tissues as well as a reference for the treatment of other tumors. |
| first_indexed | 2024-03-12T20:23:46Z |
| format | Article |
| id | doaj.art-c28ae5c8e430410c906ea88889c29354 |
| institution | Directory Open Access Journal |
| issn | 1931-7573 1556-276X |
| language | English |
| last_indexed | 2024-03-12T20:23:46Z |
| publishDate | 2019-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-c28ae5c8e430410c906ea88889c293542023-08-02T00:43:21ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2019-03-0114111410.1186/s11671-019-2882-5Improving Magnetofection of Magnetic Polyethylenimine Nanoparticles into MG-63 Osteoblasts Using a Novel Uniform Magnetic FieldChaode Cen0Jun Wu1Yong Zhang2Cong Luo3Lina Xie4Xin Zhang5Xiaolan Yang6Ming Li7Yang Bi8Tingyu Li9Tongchuan He10Department of Orthopedics, Guizhou Provincial Orthopedics HospitalDepartment of Orthopedics, Laboratory of Orthopedic Biomaterials, Children’s Hospital of Chongqing Medical UniversityDepartment of Gynaecology, The First People’s Hospital of GuiyangDepartment of Orthopedics, Laboratory of Orthopedic Biomaterials, Children’s Hospital of Chongqing Medical UniversityDepartment of Orthopedics, Laboratory of Orthopedic Biomaterials, Children’s Hospital of Chongqing Medical UniversityDepartment of Orthopedics, Laboratory of Orthopedic Biomaterials, Children’s Hospital of Chongqing Medical UniversityMinistry of Education Key Laboratory of Clinical Diagnostics, Department of Chemistry, Chongqing Medical UniversityDepartment of Orthopedics, Laboratory of Orthopedic Biomaterials, Children’s Hospital of Chongqing Medical UniversityMinistry of Education Key Laboratory of Child Development and Disorders, Chongqing Engineering Research Center of Stem Cell Therapy, China International Science and Technology Cooperation base of Child Development and Critical DisordersMinistry of Education Key Laboratory of Child Development and Disorders, Chongqing Engineering Research Center of Stem Cell Therapy, China International Science and Technology Cooperation base of Child Development and Critical DisordersLaboratory of Molecular Oncology, Department of Surgery/Orthopedics Center, The University of Chicago Medical CenterAbstract This study aimed to improve the magnetofection of MG-63 osteoblasts by integrating the use of a novel uniform magnetic field with low molecular weight polyethylenimine modified superparamagnetic iron oxide nanoparticles (PEI-SPIO-NPs). The excellent characteristics of PEI-SPIO-NPs such as size, zeta potential, the pDNA binding and protective ability were determined to be suitable for gene delivery. The novel uniform magnetic field enabled polyethylenimine-modified superparamagnetic iron oxide nanoparticles/pDNA complexes (PEI-SPIO-NPs/pDNA complexes) to rapidly and uniformly distribute on the surface of MG-63 cells, averting local transfection and decreasing disruption of the membrane caused by the centralization of positively charged PEI-SPIO-NPs, thereby increasing the effective coverage of magnetic gene carriers during transfection, and improving magnetofection efficiency. This innovative uniform magnetic field can be used to determine the optimal amount between PEI-SPIO-NPs and pDNA, as well as screen for the optimal formulation design of magnetic gene carrier under the homogenous conditions. Most importantly, the novel uniform magnetic field facilitates the transfection of PEI-SPIO-NPs/pDNA into osteoblasts, thereby providing a novel approach for the targeted delivery of therapeutic genes to osteosarcoma tissues as well as a reference for the treatment of other tumors.http://link.springer.com/article/10.1186/s11671-019-2882-5MagnetofectionMagnetic nanoparticlesUniform magnetic fieldPolyethylenimineNon-viral gene delivery |
| spellingShingle | Chaode Cen Jun Wu Yong Zhang Cong Luo Lina Xie Xin Zhang Xiaolan Yang Ming Li Yang Bi Tingyu Li Tongchuan He Improving Magnetofection of Magnetic Polyethylenimine Nanoparticles into MG-63 Osteoblasts Using a Novel Uniform Magnetic Field Nanoscale Research Letters Magnetofection Magnetic nanoparticles Uniform magnetic field Polyethylenimine Non-viral gene delivery |
| title | Improving Magnetofection of Magnetic Polyethylenimine Nanoparticles into MG-63 Osteoblasts Using a Novel Uniform Magnetic Field |
| title_full | Improving Magnetofection of Magnetic Polyethylenimine Nanoparticles into MG-63 Osteoblasts Using a Novel Uniform Magnetic Field |
| title_fullStr | Improving Magnetofection of Magnetic Polyethylenimine Nanoparticles into MG-63 Osteoblasts Using a Novel Uniform Magnetic Field |
| title_full_unstemmed | Improving Magnetofection of Magnetic Polyethylenimine Nanoparticles into MG-63 Osteoblasts Using a Novel Uniform Magnetic Field |
| title_short | Improving Magnetofection of Magnetic Polyethylenimine Nanoparticles into MG-63 Osteoblasts Using a Novel Uniform Magnetic Field |
| title_sort | improving magnetofection of magnetic polyethylenimine nanoparticles into mg 63 osteoblasts using a novel uniform magnetic field |
| topic | Magnetofection Magnetic nanoparticles Uniform magnetic field Polyethylenimine Non-viral gene delivery |
| url | http://link.springer.com/article/10.1186/s11671-019-2882-5 |
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