Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo
Equine herpesvirus type 1 (EHV-1) poses a global threat to equines. The anticancer agent berbamine (BBM), a bioactive alkaloid, has been shown to inhibit viral infection. However, whether BBM can inhibit EHV-1 infection remains unclear. This study investigated the effect of BBM treatment on EHV-1 in...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1163780/full |
| _version_ | 1797821037849608192 |
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| author | Zeyu Li Yuanxiu He Lijuan Ge Ran Quan Junzhen Chen Yue Hu Ruixue Sa Jianhua Liu Duoliang Ran Qiang Fu Huijun Shi |
| author_facet | Zeyu Li Yuanxiu He Lijuan Ge Ran Quan Junzhen Chen Yue Hu Ruixue Sa Jianhua Liu Duoliang Ran Qiang Fu Huijun Shi |
| author_sort | Zeyu Li |
| collection | DOAJ |
| description | Equine herpesvirus type 1 (EHV-1) poses a global threat to equines. The anticancer agent berbamine (BBM), a bioactive alkaloid, has been shown to inhibit viral infection. However, whether BBM can inhibit EHV-1 infection remains unclear. This study investigated the effect of BBM treatment on EHV-1 infection. Quantitative PCR (qPCR), immunoblotting, the Reed-Muench method, and pathological examination were employed to study the ability of BBM to inhibit EHV-1 infection, viral DNA replication, viral protein production, virion secretion, and cytopathogenesis in vitro and in vivo. The in vitro studies revealed that 10 μM BBM effectively suppressed EHV-1 viral entry into cells, viral DNA replication, and virion secretion, while the in vivo studies verified the ability of BBM to suppress EHV-1-induced damage of brain and lung tissues and animal mortality. These findings strongly suggest that BBM could be a serious contender in the therapeutic control of EHV-1 infection of equines. |
| first_indexed | 2024-03-13T09:46:51Z |
| format | Article |
| id | doaj.art-c28c1b5bdbe542edad8d694996dcd2ab |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-13T09:46:51Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-c28c1b5bdbe542edad8d694996dcd2ab2023-05-25T04:13:36ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-05-011010.3389/fvets.2023.11637801163780Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivoZeyu LiYuanxiu HeLijuan GeRan QuanJunzhen ChenYue HuRuixue SaJianhua LiuDuoliang RanQiang FuHuijun ShiEquine herpesvirus type 1 (EHV-1) poses a global threat to equines. The anticancer agent berbamine (BBM), a bioactive alkaloid, has been shown to inhibit viral infection. However, whether BBM can inhibit EHV-1 infection remains unclear. This study investigated the effect of BBM treatment on EHV-1 infection. Quantitative PCR (qPCR), immunoblotting, the Reed-Muench method, and pathological examination were employed to study the ability of BBM to inhibit EHV-1 infection, viral DNA replication, viral protein production, virion secretion, and cytopathogenesis in vitro and in vivo. The in vitro studies revealed that 10 μM BBM effectively suppressed EHV-1 viral entry into cells, viral DNA replication, and virion secretion, while the in vivo studies verified the ability of BBM to suppress EHV-1-induced damage of brain and lung tissues and animal mortality. These findings strongly suggest that BBM could be a serious contender in the therapeutic control of EHV-1 infection of equines.https://www.frontiersin.org/articles/10.3389/fvets.2023.1163780/fullberbamineequine herpesvirus-1 (EHV-1)Syrian hamstersvirus replicationtissue injury |
| spellingShingle | Zeyu Li Yuanxiu He Lijuan Ge Ran Quan Junzhen Chen Yue Hu Ruixue Sa Jianhua Liu Duoliang Ran Qiang Fu Huijun Shi Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo Frontiers in Veterinary Science berbamine equine herpesvirus-1 (EHV-1) Syrian hamsters virus replication tissue injury |
| title | Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo |
| title_full | Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo |
| title_fullStr | Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo |
| title_full_unstemmed | Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo |
| title_short | Berbamine, a bioactive alkaloid, suppresses equine herpesvirus type 1 in vitro and in vivo |
| title_sort | berbamine a bioactive alkaloid suppresses equine herpesvirus type 1 in vitro and in vivo |
| topic | berbamine equine herpesvirus-1 (EHV-1) Syrian hamsters virus replication tissue injury |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1163780/full |
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