Role of Daptomycin in the Induction and Persistence of the Viable but Non-Culturable State of Staphylococcus Aureus Biofilms
We have recently demonstrated that antibiotic pressure can induce the viable but non-culturable (VBNC) state in Staphylococcus aureus biofilms. Since dormant bacterial cells can undermine anti-infective therapy, a greater understanding of the role of antibiotics of last resort, including daptomycin,...
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MDPI AG
2014-09-01
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| Series: | Pathogens |
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| Online Access: | http://www.mdpi.com/2076-0817/3/3/759 |
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| author | Sonia Pasquaroli Barbara Citterio Andrea Di Cesare Mehdi Amiri Anita Manti Claudia Vuotto Francesca Biavasco |
| author_facet | Sonia Pasquaroli Barbara Citterio Andrea Di Cesare Mehdi Amiri Anita Manti Claudia Vuotto Francesca Biavasco |
| author_sort | Sonia Pasquaroli |
| collection | DOAJ |
| description | We have recently demonstrated that antibiotic pressure can induce the viable but non-culturable (VBNC) state in Staphylococcus aureus biofilms. Since dormant bacterial cells can undermine anti-infective therapy, a greater understanding of the role of antibiotics of last resort, including daptomycin, is crucial. Methicillin-resistant S. aureus 10850 biofilms were maintained on non-nutrient (NN) agar in the presence or absence of the MIC of daptomycin until loss of culturability. Viable cells were monitored by epifluorescence microscopy and flow cytometry for 150 days. All biofilms reached non-culturability at 40 days and showed a similar amount of viable cells; however, in biofilms exposed to daptomycin, their number remained unchanged throughout the experiment, whereas in those maintained on NN agar alone, no viable cells were detected after 150 days. Gene expression assays showed that after achievement of non-culturability, 16S rDNA and mecA were expressed by all biofilms, whereas glt expression was found only in daptomycin-exposed biofilms. Our findings suggest that low daptomycin concentrations, such as those that are likely to obtain within biofilms, can influence the viability and gene expression of non-culturable S. aureus cells. Resuscitation experiments are needed to establish the VBNC state of daptomycin-exposed biofilms. |
| first_indexed | 2024-04-11T13:17:28Z |
| format | Article |
| id | doaj.art-c290260249b640b8973a5149b6786e7c |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-11T13:17:28Z |
| publishDate | 2014-09-01 |
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| series | Pathogens |
| spelling | doaj.art-c290260249b640b8973a5149b6786e7c2022-12-22T04:22:22ZengMDPI AGPathogens2076-08172014-09-013375976810.3390/pathogens3030759pathogens3030759Role of Daptomycin in the Induction and Persistence of the Viable but Non-Culturable State of Staphylococcus Aureus BiofilmsSonia Pasquaroli0Barbara Citterio1Andrea Di Cesare2Mehdi Amiri3Anita Manti4Claudia Vuotto5Francesca Biavasco6Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona 60131, ItalyDepartment of Biomolecular Sciences, Sect. Toxicological, Hygiene, and Environmental Sciences, University of Urbino Carlo Bo, Urbino 61029, ItalyDepartment of Life and Environmental Sciences, Polytechnic University of Marche, Ancona 60131, ItalyDepartment of Life and Environmental Sciences, Polytechnic University of Marche, Ancona 60131, ItalyDepartment of Earth, Life and Environmental Sciences, University of Urbino Carlo Bo, Urbino 61029, ItalyMicrobial Biofilm Laboratory, IRCCS Fondazione Santa Lucia, Rome 00179, ItalyDepartment of Life and Environmental Sciences, Polytechnic University of Marche, Ancona 60131, ItalyWe have recently demonstrated that antibiotic pressure can induce the viable but non-culturable (VBNC) state in Staphylococcus aureus biofilms. Since dormant bacterial cells can undermine anti-infective therapy, a greater understanding of the role of antibiotics of last resort, including daptomycin, is crucial. Methicillin-resistant S. aureus 10850 biofilms were maintained on non-nutrient (NN) agar in the presence or absence of the MIC of daptomycin until loss of culturability. Viable cells were monitored by epifluorescence microscopy and flow cytometry for 150 days. All biofilms reached non-culturability at 40 days and showed a similar amount of viable cells; however, in biofilms exposed to daptomycin, their number remained unchanged throughout the experiment, whereas in those maintained on NN agar alone, no viable cells were detected after 150 days. Gene expression assays showed that after achievement of non-culturability, 16S rDNA and mecA were expressed by all biofilms, whereas glt expression was found only in daptomycin-exposed biofilms. Our findings suggest that low daptomycin concentrations, such as those that are likely to obtain within biofilms, can influence the viability and gene expression of non-culturable S. aureus cells. Resuscitation experiments are needed to establish the VBNC state of daptomycin-exposed biofilms.http://www.mdpi.com/2076-0817/3/3/759Staphylococcus aureus biofilmVBNCdaptomycin |
| spellingShingle | Sonia Pasquaroli Barbara Citterio Andrea Di Cesare Mehdi Amiri Anita Manti Claudia Vuotto Francesca Biavasco Role of Daptomycin in the Induction and Persistence of the Viable but Non-Culturable State of Staphylococcus Aureus Biofilms Pathogens Staphylococcus aureus biofilm VBNC daptomycin |
| title | Role of Daptomycin in the Induction and Persistence of the Viable but Non-Culturable State of Staphylococcus Aureus Biofilms |
| title_full | Role of Daptomycin in the Induction and Persistence of the Viable but Non-Culturable State of Staphylococcus Aureus Biofilms |
| title_fullStr | Role of Daptomycin in the Induction and Persistence of the Viable but Non-Culturable State of Staphylococcus Aureus Biofilms |
| title_full_unstemmed | Role of Daptomycin in the Induction and Persistence of the Viable but Non-Culturable State of Staphylococcus Aureus Biofilms |
| title_short | Role of Daptomycin in the Induction and Persistence of the Viable but Non-Culturable State of Staphylococcus Aureus Biofilms |
| title_sort | role of daptomycin in the induction and persistence of the viable but non culturable state of staphylococcus aureus biofilms |
| topic | Staphylococcus aureus biofilm VBNC daptomycin |
| url | http://www.mdpi.com/2076-0817/3/3/759 |
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