Concomitant Infections With Canine Parvovirus Type 2 and Intracellular Tick-Borne Pathogens in Two Puppy Dogs

In this report the concomitant infection with canine parvovirus type 2 (CPV-2), Hepatozoon canis and Ehrlichia canis in two puppy dogs from Southern Italy is described. Dogs were referred to a veterinary university hospital for the acute onset of lethargy and gastrointestinal signs. A complete clini...

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Main Authors: Lorenza Urbani, Alessandro Tirolo, Andrea Balboni, Roberta Troia, Francesco Dondi, Mara Battilani
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-07-01
Series:Frontiers in Veterinary Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2022.964177/full
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author Lorenza Urbani
Alessandro Tirolo
Andrea Balboni
Roberta Troia
Francesco Dondi
Mara Battilani
author_facet Lorenza Urbani
Alessandro Tirolo
Andrea Balboni
Roberta Troia
Francesco Dondi
Mara Battilani
author_sort Lorenza Urbani
collection DOAJ
description In this report the concomitant infection with canine parvovirus type 2 (CPV-2), Hepatozoon canis and Ehrlichia canis in two puppy dogs from Southern Italy is described. Dogs were referred to a veterinary university hospital for the acute onset of lethargy and gastrointestinal signs. A complete clinical and clinicopathological evaluation was carried out and the multiple infection was confirmed by microscopic detection of inclusion bodies in peripheral blood smear, rapid immunoenzymatic tests, indirect fluorescent antibody tests, and molecular assays. Sequence analysis revealed that the CPV-2 identified belonged to the 2c variant and had amino acid residues in the predicted VP2 protein typical of “Asian-like” strains widespread in Asia and occasionally reported in Romania, Nigeria and Italy, particularly in the region of Sicily. Numerous monocytes were infected by both H. canis gamonts and E. canis morulae, suggesting that this co-infection is not accidental and that E. canis preferably infects those cells parasitized by H. canis. The clinical presentation of these animals was severe but supportive cares associated with early etiological therapy allowed a good prognosis. Movement of puppies from geographic areas where vector-borne pathogens are endemic must be carefully evaluated and core vaccinations and ectoparasite prevention treatments must be rigorously adopted.
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spelling doaj.art-c290ca7c3cf945948e3016255e38ddc02022-12-22T01:53:33ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-07-01910.3389/fvets.2022.964177964177Concomitant Infections With Canine Parvovirus Type 2 and Intracellular Tick-Borne Pathogens in Two Puppy DogsLorenza UrbaniAlessandro TiroloAndrea BalboniRoberta TroiaFrancesco DondiMara BattilaniIn this report the concomitant infection with canine parvovirus type 2 (CPV-2), Hepatozoon canis and Ehrlichia canis in two puppy dogs from Southern Italy is described. Dogs were referred to a veterinary university hospital for the acute onset of lethargy and gastrointestinal signs. A complete clinical and clinicopathological evaluation was carried out and the multiple infection was confirmed by microscopic detection of inclusion bodies in peripheral blood smear, rapid immunoenzymatic tests, indirect fluorescent antibody tests, and molecular assays. Sequence analysis revealed that the CPV-2 identified belonged to the 2c variant and had amino acid residues in the predicted VP2 protein typical of “Asian-like” strains widespread in Asia and occasionally reported in Romania, Nigeria and Italy, particularly in the region of Sicily. Numerous monocytes were infected by both H. canis gamonts and E. canis morulae, suggesting that this co-infection is not accidental and that E. canis preferably infects those cells parasitized by H. canis. The clinical presentation of these animals was severe but supportive cares associated with early etiological therapy allowed a good prognosis. Movement of puppies from geographic areas where vector-borne pathogens are endemic must be carefully evaluated and core vaccinations and ectoparasite prevention treatments must be rigorously adopted.https://www.frontiersin.org/articles/10.3389/fvets.2022.964177/fullco-infectiondogEhrlichia canisHepatozoon canisparvovirus enteritisvector-borne disease
spellingShingle Lorenza Urbani
Alessandro Tirolo
Andrea Balboni
Roberta Troia
Francesco Dondi
Mara Battilani
Concomitant Infections With Canine Parvovirus Type 2 and Intracellular Tick-Borne Pathogens in Two Puppy Dogs
Frontiers in Veterinary Science
co-infection
dog
Ehrlichia canis
Hepatozoon canis
parvovirus enteritis
vector-borne disease
title Concomitant Infections With Canine Parvovirus Type 2 and Intracellular Tick-Borne Pathogens in Two Puppy Dogs
title_full Concomitant Infections With Canine Parvovirus Type 2 and Intracellular Tick-Borne Pathogens in Two Puppy Dogs
title_fullStr Concomitant Infections With Canine Parvovirus Type 2 and Intracellular Tick-Borne Pathogens in Two Puppy Dogs
title_full_unstemmed Concomitant Infections With Canine Parvovirus Type 2 and Intracellular Tick-Borne Pathogens in Two Puppy Dogs
title_short Concomitant Infections With Canine Parvovirus Type 2 and Intracellular Tick-Borne Pathogens in Two Puppy Dogs
title_sort concomitant infections with canine parvovirus type 2 and intracellular tick borne pathogens in two puppy dogs
topic co-infection
dog
Ehrlichia canis
Hepatozoon canis
parvovirus enteritis
vector-borne disease
url https://www.frontiersin.org/articles/10.3389/fvets.2022.964177/full
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