One-Step Induction of Neurons from Mouse Embryonic Stem Cells in Serum-Free Media Containing Vitamin B and Heparin

One-Step Induction of Neurons from Mouse Embryonic Stem Cells in Serum-Free Media Containing Vitamin B and Heparin

We present a simple method for neural cell fate specification directly from mouse embryonic stem cells (ES cells) in serum-free conditions in the absence of embryoid body formation. Dissociated ES cells were cultured in serum-free media supplemented with vitamin B 12 and heparin, but without any exp...

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Bibliographic Details
Main Authors: Hironori Yamazoe, Masato Kobori, Yoshinobu Murakami, Keiichi Yano, Mitsuo Satoh, Kenji Mizuseki, Yoshiki Sasai, Hiroo Iwata
Format: Article
Language:English
Published: SAGE Publishing 2006-02-01
Series:Cell Transplantation
Online Access:https://doi.org/10.3727/000000006783982061

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