Effect of Scutellarin on Melanoma B16 in Vitro and in Vivo and Its Mechanism
The aim of the present study was to clarify the pathway and mechanism involved in the anti-melanona effect of Scutellarin (SCU). CCK-8 and Transwell assays were employed to evaluate the cell proliferation and migratory abilities of B16 in vitro. Then, a B16 tumor-bearing mouse model was established...
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| Format: | Article |
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The editorial department of Science and Technology of Food Industry
2023-09-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110033 |
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| author | Chongchong TIAN Qi ZHANG Xiaobo BAO Yanshen CHEN |
| author_facet | Chongchong TIAN Qi ZHANG Xiaobo BAO Yanshen CHEN |
| author_sort | Chongchong TIAN |
| collection | DOAJ |
| description | The aim of the present study was to clarify the pathway and mechanism involved in the anti-melanona effect of Scutellarin (SCU). CCK-8 and Transwell assays were employed to evaluate the cell proliferation and migratory abilities of B16 in vitro. Then, a B16 tumor-bearing mouse model was established to explore the effect of SCU on tumor growth in vivo. Immunohistochemistry and flow cytometry were also used to examine the effect of SCU on tumor angiogenesis and the mobilization of bone marrow-derived cells, respectively. The results showed that SCU substantially decreased the proliferation of B16 cells at doses of 160, 320, and 640 μmol/L, respectively (P<0.01). Moreover, the migration and invasion capacity of B16 cells were apparently reduced when treated with 160 and 320 μmol/L SCU. Besides, the growth of B16 cells in vivo was remarably slower after administration of 30 and 60 mg/kg SCU (P<0.01). Further immunohistochemistry semi-quantitative results revealed the microvascular density (MVD) of B16 tumor tissues was significantly decreased (P<0.01) and flow cytometry data shown that SCU could lower the cell counts of vascular endothelial growth factor receptor 2 positive (VEGFR2+) bone marrow-derived cells (BMDCs) and Gr-1+CD11b+BMDCs in the peripheral circulating blood of tumor-bearing mice (P<0.01). In conclusion, SCU could inhibit the growth of B16 tumor cells in vitro and in vivo, which may be directly related to its inhibition of tumor cell function, as well as its inhibition of BMDCs mobilization, thus indirectly reducing tumor angiogenesis. |
| first_indexed | 2024-03-12T13:35:48Z |
| format | Article |
| id | doaj.art-c29599070130448b87356d5032be2a30 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T13:35:48Z |
| publishDate | 2023-09-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-c29599070130448b87356d5032be2a302023-08-24T05:51:58ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-09-01441740641210.13386/j.issn1002-0306.20221100332022110033-17Effect of Scutellarin on Melanoma B16 in Vitro and in Vivo and Its MechanismChongchong TIAN0Qi ZHANG1Xiaobo BAO2Yanshen CHEN3Department of Pharmacy, Jiangsu Vocational College of Medicine, Yancheng 224005, ChinaDepartment of Pharmacy, Jiangsu Vocational College of Medicine, Yancheng 224005, ChinaDepartment of Pharmacy, Jiangsu Vocational College of Medicine, Yancheng 224005, ChinaDepartment of Pharmacy, Jiangsu Vocational College of Medicine, Yancheng 224005, ChinaThe aim of the present study was to clarify the pathway and mechanism involved in the anti-melanona effect of Scutellarin (SCU). CCK-8 and Transwell assays were employed to evaluate the cell proliferation and migratory abilities of B16 in vitro. Then, a B16 tumor-bearing mouse model was established to explore the effect of SCU on tumor growth in vivo. Immunohistochemistry and flow cytometry were also used to examine the effect of SCU on tumor angiogenesis and the mobilization of bone marrow-derived cells, respectively. The results showed that SCU substantially decreased the proliferation of B16 cells at doses of 160, 320, and 640 μmol/L, respectively (P<0.01). Moreover, the migration and invasion capacity of B16 cells were apparently reduced when treated with 160 and 320 μmol/L SCU. Besides, the growth of B16 cells in vivo was remarably slower after administration of 30 and 60 mg/kg SCU (P<0.01). Further immunohistochemistry semi-quantitative results revealed the microvascular density (MVD) of B16 tumor tissues was significantly decreased (P<0.01) and flow cytometry data shown that SCU could lower the cell counts of vascular endothelial growth factor receptor 2 positive (VEGFR2+) bone marrow-derived cells (BMDCs) and Gr-1+CD11b+BMDCs in the peripheral circulating blood of tumor-bearing mice (P<0.01). In conclusion, SCU could inhibit the growth of B16 tumor cells in vitro and in vivo, which may be directly related to its inhibition of tumor cell function, as well as its inhibition of BMDCs mobilization, thus indirectly reducing tumor angiogenesis.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110033scutellarinmelanomamigration and invasionangiogenesis |
| spellingShingle | Chongchong TIAN Qi ZHANG Xiaobo BAO Yanshen CHEN Effect of Scutellarin on Melanoma B16 in Vitro and in Vivo and Its Mechanism Shipin gongye ke-ji scutellarin melanoma migration and invasion angiogenesis |
| title | Effect of Scutellarin on Melanoma B16 in Vitro and in Vivo and Its Mechanism |
| title_full | Effect of Scutellarin on Melanoma B16 in Vitro and in Vivo and Its Mechanism |
| title_fullStr | Effect of Scutellarin on Melanoma B16 in Vitro and in Vivo and Its Mechanism |
| title_full_unstemmed | Effect of Scutellarin on Melanoma B16 in Vitro and in Vivo and Its Mechanism |
| title_short | Effect of Scutellarin on Melanoma B16 in Vitro and in Vivo and Its Mechanism |
| title_sort | effect of scutellarin on melanoma b16 in vitro and in vivo and its mechanism |
| topic | scutellarin melanoma migration and invasion angiogenesis |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022110033 |
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