Mycoremediation of Soils Polluted with Trichloroethylene: First Evidence of <i>Pleurotus</i> Genus Effectiveness
Trichloroethylene (TCE) is a proven carcinogenic chlorinated organic compound widely used as a solvent in industrial cleaning solutions; it is easily found in the soil, air, and water and is a hazardous environmental pollutant. Most studies have attempted to remove TCE from air and water using diffe...
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MDPI AG
2021-02-01
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| Online Access: | https://www.mdpi.com/2076-3417/11/4/1354 |
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| author | Begoña Mayans Raquel Camacho-Arévalo Carlos García-Delgado Cynthia Alcántara Norbert Nägele Rafael Antón-Herrero Consuelo Escolástico Enrique Eymar |
| author_facet | Begoña Mayans Raquel Camacho-Arévalo Carlos García-Delgado Cynthia Alcántara Norbert Nägele Rafael Antón-Herrero Consuelo Escolástico Enrique Eymar |
| author_sort | Begoña Mayans |
| collection | DOAJ |
| description | Trichloroethylene (TCE) is a proven carcinogenic chlorinated organic compound widely used as a solvent in industrial cleaning solutions; it is easily found in the soil, air, and water and is a hazardous environmental pollutant. Most studies have attempted to remove TCE from air and water using different anaerobic bacteria species. In addition, a few have used white-rot fungi, although there are hardly any in soil. The objective of the present work is to assess TCE removal efficiency using two species of the genus <i>Pleurotus</i> that have not been tested before: <i>Pleurotus ostreatus</i> and <i>Pleurotus eryngii</i>, growing on a sandy loam soil. These fungi presented different intra- and extracellular enzymatic systems (chytochrome P450 (CYP450), laccase, Mn peroxidase (MnP)) capable of aerobically degrading TCE to less harmful compounds. The potential toxicity of TCE to <i>P. ostreatus</i> and <i>P. eryngii</i> was firstly tested in a TCE-spiked liquid broth (70 mg L<sup>−1</sup> and 140 mg L<sup>−1</sup>) for 14 days. Then, both fungi were assessed for their ability to degrade the pollutant in sandy loam soil spiked with 140 mg kg<sup>−1</sup> of TCE. <i>P. ostreatus</i> and <i>P. eryngii</i> improved the natural dissipation of TCE from soil by 44%. Extracellular enzymes were poorly expressed, but mainly in the presence of the contaminant, in accordance with the hypothesis of the involvement of CYP450. |
| first_indexed | 2024-03-09T06:00:32Z |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T06:00:32Z |
| publishDate | 2021-02-01 |
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| series | Applied Sciences |
| spelling | doaj.art-c29b706776e540cd88e7f89310dc07682023-12-03T12:10:05ZengMDPI AGApplied Sciences2076-34172021-02-01114135410.3390/app11041354Mycoremediation of Soils Polluted with Trichloroethylene: First Evidence of <i>Pleurotus</i> Genus EffectivenessBegoña Mayans0Raquel Camacho-Arévalo1Carlos García-Delgado2Cynthia Alcántara3Norbert Nägele4Rafael Antón-Herrero5Consuelo Escolástico6Enrique Eymar7Department of Agricultural Chemistry and Food Sciences, Autonomous University of Madrid, 28049 Madrid, SpainDepartment of Agricultural Chemistry and Food Sciences, Autonomous University of Madrid, 28049 Madrid, SpainDepartment of Geology and Geochemistry, Autonomous University of Madrid, 28049 Madrid, SpainKepler Ingeniería y Ecogestión, S.L, 09001 Burgos, SpainKepler Ingeniería y Ecogestión, S.L, 09001 Burgos, SpainDepartment of Agricultural Chemistry and Food Sciences, Autonomous University of Madrid, 28049 Madrid, SpainDepartment of Organic and Bio-organic Chemistry, Faculty of Sciences, Universidad Nacional de Educación a Distancia, 28040 Madrid, SpainDepartment of Agricultural Chemistry and Food Sciences, Autonomous University of Madrid, 28049 Madrid, SpainTrichloroethylene (TCE) is a proven carcinogenic chlorinated organic compound widely used as a solvent in industrial cleaning solutions; it is easily found in the soil, air, and water and is a hazardous environmental pollutant. Most studies have attempted to remove TCE from air and water using different anaerobic bacteria species. In addition, a few have used white-rot fungi, although there are hardly any in soil. The objective of the present work is to assess TCE removal efficiency using two species of the genus <i>Pleurotus</i> that have not been tested before: <i>Pleurotus ostreatus</i> and <i>Pleurotus eryngii</i>, growing on a sandy loam soil. These fungi presented different intra- and extracellular enzymatic systems (chytochrome P450 (CYP450), laccase, Mn peroxidase (MnP)) capable of aerobically degrading TCE to less harmful compounds. The potential toxicity of TCE to <i>P. ostreatus</i> and <i>P. eryngii</i> was firstly tested in a TCE-spiked liquid broth (70 mg L<sup>−1</sup> and 140 mg L<sup>−1</sup>) for 14 days. Then, both fungi were assessed for their ability to degrade the pollutant in sandy loam soil spiked with 140 mg kg<sup>−1</sup> of TCE. <i>P. ostreatus</i> and <i>P. eryngii</i> improved the natural dissipation of TCE from soil by 44%. Extracellular enzymes were poorly expressed, but mainly in the presence of the contaminant, in accordance with the hypothesis of the involvement of CYP450.https://www.mdpi.com/2076-3417/11/4/1354trichloroethylenemycoremediation<i>Pleurotus</i>bioremediationbiodegradationpollution |
| spellingShingle | Begoña Mayans Raquel Camacho-Arévalo Carlos García-Delgado Cynthia Alcántara Norbert Nägele Rafael Antón-Herrero Consuelo Escolástico Enrique Eymar Mycoremediation of Soils Polluted with Trichloroethylene: First Evidence of <i>Pleurotus</i> Genus Effectiveness Applied Sciences trichloroethylene mycoremediation <i>Pleurotus</i> bioremediation biodegradation pollution |
| title | Mycoremediation of Soils Polluted with Trichloroethylene: First Evidence of <i>Pleurotus</i> Genus Effectiveness |
| title_full | Mycoremediation of Soils Polluted with Trichloroethylene: First Evidence of <i>Pleurotus</i> Genus Effectiveness |
| title_fullStr | Mycoremediation of Soils Polluted with Trichloroethylene: First Evidence of <i>Pleurotus</i> Genus Effectiveness |
| title_full_unstemmed | Mycoremediation of Soils Polluted with Trichloroethylene: First Evidence of <i>Pleurotus</i> Genus Effectiveness |
| title_short | Mycoremediation of Soils Polluted with Trichloroethylene: First Evidence of <i>Pleurotus</i> Genus Effectiveness |
| title_sort | mycoremediation of soils polluted with trichloroethylene first evidence of i pleurotus i genus effectiveness |
| topic | trichloroethylene mycoremediation <i>Pleurotus</i> bioremediation biodegradation pollution |
| url | https://www.mdpi.com/2076-3417/11/4/1354 |
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