Gender-specific characteristics of hypertrophic response in cardiomyocytes derived from human embryonic stem cells
Introduction: Gender-specific phenotypes of the heart were reported with respect to both physiology and pathology. While most differences were associated with the sex hormones, differential expression of genes received special attention, particularly X-Y chromosomes’ genes. Methods: Here, we compare...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2021-05-01
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| Series: | Journal of Cardiovascular and Thoracic Research |
| Subjects: | |
| Online Access: | https://jcvtr.tbzmed.ac.ir/PDF/jcvtr-13-146.pdf |
| _version_ | 1818849632951730176 |
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| author | Shiva Ahmadvand Ali Osia Anna Meyfour Sara Pahlavan |
| author_facet | Shiva Ahmadvand Ali Osia Anna Meyfour Sara Pahlavan |
| author_sort | Shiva Ahmadvand |
| collection | DOAJ |
| description | Introduction: Gender-specific phenotypes of the heart were reported with respect to both physiology and pathology. While most differences were associated with the sex hormones, differential expression of genes received special attention, particularly X-Y chromosomes’ genes. Methods: Here, we compared cardiogenesis by gene expression analysis of lineage specific markers and X-Y chromosomes’ genes, during in vitro differentiation of XY and XX human embryonic stem cells (hESC), in a hormone-free setup. Results: Downregulation of pluripotency marker (NANOG) and upregulation of cardiac mesoderm and progenitor markers (GATA4, TBX5, NKX2.5, ISL1) was remained temporally similar in differentiating XY and XX hESCs. Isoproterenol treatment of XY and XX hESC-derived cardiomyocytes (hESCCM) induced hypertrophy in a sex-specific manner, with female cardiomyocytes showing response at higher isoproterenol concentration and a later time point of differentiation. Interestingly, KDM5C as an X-linked gene, was markedly upregulated in both hypertrophied male and female cardiomyocytes. Conclusion: Collectively, our results indicated a temporally identical cardiogenesis, but more susceptibility of XY hESC-CM to hypertrophic stimulus in a hormone-free condition. |
| first_indexed | 2024-12-19T06:36:20Z |
| format | Article |
| id | doaj.art-c29d8c6322ac4f5eb950a581fcbf4d6d |
| institution | Directory Open Access Journal |
| issn | 2008-5117 2008-6830 |
| language | English |
| last_indexed | 2024-12-19T06:36:20Z |
| publishDate | 2021-05-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Journal of Cardiovascular and Thoracic Research |
| spelling | doaj.art-c29d8c6322ac4f5eb950a581fcbf4d6d2022-12-21T20:32:14ZengTabriz University of Medical SciencesJournal of Cardiovascular and Thoracic Research2008-51172008-68302021-05-0113214615510.34172/jcvtr.2021.32jcvtr-30194Gender-specific characteristics of hypertrophic response in cardiomyocytes derived from human embryonic stem cellsShiva Ahmadvand0Ali Osia1Anna Meyfour2Sara Pahlavan3Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranCobel Darou, Tehran, IranBasic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranIntroduction: Gender-specific phenotypes of the heart were reported with respect to both physiology and pathology. While most differences were associated with the sex hormones, differential expression of genes received special attention, particularly X-Y chromosomes’ genes. Methods: Here, we compared cardiogenesis by gene expression analysis of lineage specific markers and X-Y chromosomes’ genes, during in vitro differentiation of XY and XX human embryonic stem cells (hESC), in a hormone-free setup. Results: Downregulation of pluripotency marker (NANOG) and upregulation of cardiac mesoderm and progenitor markers (GATA4, TBX5, NKX2.5, ISL1) was remained temporally similar in differentiating XY and XX hESCs. Isoproterenol treatment of XY and XX hESC-derived cardiomyocytes (hESCCM) induced hypertrophy in a sex-specific manner, with female cardiomyocytes showing response at higher isoproterenol concentration and a later time point of differentiation. Interestingly, KDM5C as an X-linked gene, was markedly upregulated in both hypertrophied male and female cardiomyocytes. Conclusion: Collectively, our results indicated a temporally identical cardiogenesis, but more susceptibility of XY hESC-CM to hypertrophic stimulus in a hormone-free condition.https://jcvtr.tbzmed.ac.ir/PDF/jcvtr-13-146.pdfembryonic stem cellscardiomyocyte differentiationsexual dimorphismhypertrophy |
| spellingShingle | Shiva Ahmadvand Ali Osia Anna Meyfour Sara Pahlavan Gender-specific characteristics of hypertrophic response in cardiomyocytes derived from human embryonic stem cells Journal of Cardiovascular and Thoracic Research embryonic stem cells cardiomyocyte differentiation sexual dimorphism hypertrophy |
| title | Gender-specific characteristics of hypertrophic response in cardiomyocytes derived from human embryonic stem cells |
| title_full | Gender-specific characteristics of hypertrophic response in cardiomyocytes derived from human embryonic stem cells |
| title_fullStr | Gender-specific characteristics of hypertrophic response in cardiomyocytes derived from human embryonic stem cells |
| title_full_unstemmed | Gender-specific characteristics of hypertrophic response in cardiomyocytes derived from human embryonic stem cells |
| title_short | Gender-specific characteristics of hypertrophic response in cardiomyocytes derived from human embryonic stem cells |
| title_sort | gender specific characteristics of hypertrophic response in cardiomyocytes derived from human embryonic stem cells |
| topic | embryonic stem cells cardiomyocyte differentiation sexual dimorphism hypertrophy |
| url | https://jcvtr.tbzmed.ac.ir/PDF/jcvtr-13-146.pdf |
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