Layer Inoculation as a New Technology to Resist Volatile Fatty Acid Inhibition during Solid-State Anaerobic Digestion: Methane Yield Performance and Microbial Responses
Solid-state anaerobic digestion is easily inhibited by high volatile fatty acid induced by high total solids, although it is a promising technology. Previous studies on volatile fatty acid inhibition mainly focused on total solid content, co-digestion substrates, and external additives. The present...
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MDPI AG
2023-05-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/6/535 |
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| author | Xingliang Gao Zhuowu Li Keqiang Zhang Dewang Kong Wenxuan Gao Junfeng Liang Fuyuan Liu Lianzhu Du |
| author_facet | Xingliang Gao Zhuowu Li Keqiang Zhang Dewang Kong Wenxuan Gao Junfeng Liang Fuyuan Liu Lianzhu Du |
| author_sort | Xingliang Gao |
| collection | DOAJ |
| description | Solid-state anaerobic digestion is easily inhibited by high volatile fatty acid induced by high total solids, although it is a promising technology. Previous studies on volatile fatty acid inhibition mainly focused on total solid content, co-digestion substrates, and external additives. The present study proposed a new inoculation method named layer inoculation and compared it to premixing inoculation in the solid-state anaerobic digestion of pig manure and maize straw. The results showed that the cumulative CH<sub>4</sub> yields from layer inoculation (211.5 mL/g-VS) were 5.64 times more than premixing inoculation (37.5 mL/g-VS) under a low inoculation ratio (25%), with the values of total volatile fatty acid being greater than 30.0 mg/g. The concentrations of total VFAs and acetic acid from layer inoculation decreased dramatically during days 18–43. Layer inoculation also showed wider specific methane yield peaks and shorter startup times than premixing inoculation. <i>Methanosphaerula</i> and <i>Methanothrix</i> were the most dominant genera, while the genus <i>Methanosphaerula</i> did not correlate with volatile fatty acids, pH, or total ammonia nitrogen. The hydrogenotrophic methanogen pathway was predominant during solid-state anaerobic digestion; the shift from hydrogenotrophic to acetoclastic occurred in premixing inoculation, and it was stable in layer inoculation (61.20–68.88%). Overall, layer inoculation can effectively enhance methane production under high volatile fatty acid concentrations compared with premixing inoculation. |
| first_indexed | 2024-03-11T02:30:02Z |
| format | Article |
| id | doaj.art-c2a0220ca2944cb4b1f9cc57a6d1a764 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T02:30:02Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-c2a0220ca2944cb4b1f9cc57a6d1a7642023-11-18T10:21:05ZengMDPI AGFermentation2311-56372023-05-019653510.3390/fermentation9060535Layer Inoculation as a New Technology to Resist Volatile Fatty Acid Inhibition during Solid-State Anaerobic Digestion: Methane Yield Performance and Microbial ResponsesXingliang Gao0Zhuowu Li1Keqiang Zhang2Dewang Kong3Wenxuan Gao4Junfeng Liang5Fuyuan Liu6Lianzhu Du7State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural Reclamation Sciences, 221 Wuyi Road, Shihezi 832000, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, 31 Fukang Road, Tianjin 300191, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, 31 Fukang Road, Tianjin 300191, ChinaHangzhou Energy and Environmental Engineering Co., Ltd., 207 East Fengqi Road, Hangzhou 310020, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, 31 Fukang Road, Tianjin 300191, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, 31 Fukang Road, Tianjin 300191, ChinaState Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural Reclamation Sciences, 221 Wuyi Road, Shihezi 832000, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, 31 Fukang Road, Tianjin 300191, ChinaSolid-state anaerobic digestion is easily inhibited by high volatile fatty acid induced by high total solids, although it is a promising technology. Previous studies on volatile fatty acid inhibition mainly focused on total solid content, co-digestion substrates, and external additives. The present study proposed a new inoculation method named layer inoculation and compared it to premixing inoculation in the solid-state anaerobic digestion of pig manure and maize straw. The results showed that the cumulative CH<sub>4</sub> yields from layer inoculation (211.5 mL/g-VS) were 5.64 times more than premixing inoculation (37.5 mL/g-VS) under a low inoculation ratio (25%), with the values of total volatile fatty acid being greater than 30.0 mg/g. The concentrations of total VFAs and acetic acid from layer inoculation decreased dramatically during days 18–43. Layer inoculation also showed wider specific methane yield peaks and shorter startup times than premixing inoculation. <i>Methanosphaerula</i> and <i>Methanothrix</i> were the most dominant genera, while the genus <i>Methanosphaerula</i> did not correlate with volatile fatty acids, pH, or total ammonia nitrogen. The hydrogenotrophic methanogen pathway was predominant during solid-state anaerobic digestion; the shift from hydrogenotrophic to acetoclastic occurred in premixing inoculation, and it was stable in layer inoculation (61.20–68.88%). Overall, layer inoculation can effectively enhance methane production under high volatile fatty acid concentrations compared with premixing inoculation.https://www.mdpi.com/2311-5637/9/6/535solid-state anaerobic digestionlayer inoculationpremixing inoculationmicrobial communityvolatile fatty acid inhibition |
| spellingShingle | Xingliang Gao Zhuowu Li Keqiang Zhang Dewang Kong Wenxuan Gao Junfeng Liang Fuyuan Liu Lianzhu Du Layer Inoculation as a New Technology to Resist Volatile Fatty Acid Inhibition during Solid-State Anaerobic Digestion: Methane Yield Performance and Microbial Responses Fermentation solid-state anaerobic digestion layer inoculation premixing inoculation microbial community volatile fatty acid inhibition |
| title | Layer Inoculation as a New Technology to Resist Volatile Fatty Acid Inhibition during Solid-State Anaerobic Digestion: Methane Yield Performance and Microbial Responses |
| title_full | Layer Inoculation as a New Technology to Resist Volatile Fatty Acid Inhibition during Solid-State Anaerobic Digestion: Methane Yield Performance and Microbial Responses |
| title_fullStr | Layer Inoculation as a New Technology to Resist Volatile Fatty Acid Inhibition during Solid-State Anaerobic Digestion: Methane Yield Performance and Microbial Responses |
| title_full_unstemmed | Layer Inoculation as a New Technology to Resist Volatile Fatty Acid Inhibition during Solid-State Anaerobic Digestion: Methane Yield Performance and Microbial Responses |
| title_short | Layer Inoculation as a New Technology to Resist Volatile Fatty Acid Inhibition during Solid-State Anaerobic Digestion: Methane Yield Performance and Microbial Responses |
| title_sort | layer inoculation as a new technology to resist volatile fatty acid inhibition during solid state anaerobic digestion methane yield performance and microbial responses |
| topic | solid-state anaerobic digestion layer inoculation premixing inoculation microbial community volatile fatty acid inhibition |
| url | https://www.mdpi.com/2311-5637/9/6/535 |
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