Immunologic evaluation of extracted intestinal proteins from Angiostrongylus cantonensis adult worms
Background: To determine whether intestinal Angiostrongylus cantonensis antigens can induce protective immunity in rats, gut antigens prepared from female adults (FAGP) and somatic antigens prepared from both male (MA) and female (FA) adult worms were used to immunize rats. Methods: Rats were immuni...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-08-01
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| Series: | Journal of Microbiology, Immunology and Infection |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118217301482 |
| _version_ | 1818248432583704576 |
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| author | Chien-Yu Lee Po-Ching Cheng Chen-Hsun Ho Chia-Kwung Fan David Chao |
| author_facet | Chien-Yu Lee Po-Ching Cheng Chen-Hsun Ho Chia-Kwung Fan David Chao |
| author_sort | Chien-Yu Lee |
| collection | DOAJ |
| description | Background: To determine whether intestinal Angiostrongylus cantonensis antigens can induce protective immunity in rats, gut antigens prepared from female adults (FAGP) and somatic antigens prepared from both male (MA) and female (FA) adult worms were used to immunize rats. Methods: Rats were immunized twice with MA, FA, or FAGP antigens and then challenged with 50 third-stage A. cantonensis larvae, and different readouts were used to monitor protective immunity. Additionally, protein profiles of MA, FA, and FAGP extracts were analyzed and characterized by immunodetection methods. Results: A 15% reduction in fifth-stage larvae from brains and a 14% reduction in adult worms from pulmonary arteries were observed in rats immunized with FAGP compared to controls. However, there was a >50% reduction in rats immunized with MA or FA. The lengths of larvae and adults recovered from FAGP-immunized rats were shorter than those recovered from other groups. The number of first-stage larvae recovered from fecal material in FAGP-immunized rats was significantly reduced. Additionally, FAGP induced the highest splenocyte proliferation. Serum IgG titers were not directly correlated with protective immunity. An 84 kDa gut membrane protein was strongly recognized by anti-FAGP antibodies. Conclusion: We demonstrated that immune responses induced by FAGP reduced the growth, development, and reproduction of A. cantonensis in subsequent infections. While the possibility of using FAGP combining with MA or FA antigens as a multi-function vaccine in immune protection against A. cantonensis needs to be further elucidated, we hope that it provides a novel strategy for this parasite vaccine development. Keywords: Angiostrongylus cantonensis, Intestinal proteins, Protective immunity, Anti-fertility |
| first_indexed | 2024-12-12T15:20:30Z |
| format | Article |
| id | doaj.art-c2a217e92b6c46138155bbde85b7767c |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-12-12T15:20:30Z |
| publishDate | 2019-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-c2a217e92b6c46138155bbde85b7767c2022-12-22T00:20:24ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822019-08-01524620629Immunologic evaluation of extracted intestinal proteins from Angiostrongylus cantonensis adult wormsChien-Yu Lee0Po-Ching Cheng1Chen-Hsun Ho2Chia-Kwung Fan3David Chao4Department of Pediatrics, Tao-Yuan General Hospital, Taoyuan, Taiwan, ROCDepartment of Molecular Parasitology and Tropical Diseases, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, ROC; Center for International Tropical Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, ROC; Corresponding author. Department of Molecular Parasitology and Tropical Diseases, School of Medicine, College of Medicine, Taipei Medical University, No. 250, Wu-Hsing St., Taipei 11031, Taiwan, ROC. Fax: +886 2 22437030.Department of Urology, School of Medicine, Taipei Medical University, Taipei, Taiwan, ROC; Department of Urology, Taipei Medical University–Shuang Ho Hospital, Taipei, Taiwan, ROCDepartment of Molecular Parasitology and Tropical Diseases, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, ROC; Center for International Tropical Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, ROCDepartment of Biological Science, National Sun Yat-sen University, Kaohsiung, Taiwan, ROC; Corresponding author. Department of Biological Science, National Sun Yat-sen University, 70 Lien-hai Rd., Kaohsiung 80424, Taiwan, ROC.Background: To determine whether intestinal Angiostrongylus cantonensis antigens can induce protective immunity in rats, gut antigens prepared from female adults (FAGP) and somatic antigens prepared from both male (MA) and female (FA) adult worms were used to immunize rats. Methods: Rats were immunized twice with MA, FA, or FAGP antigens and then challenged with 50 third-stage A. cantonensis larvae, and different readouts were used to monitor protective immunity. Additionally, protein profiles of MA, FA, and FAGP extracts were analyzed and characterized by immunodetection methods. Results: A 15% reduction in fifth-stage larvae from brains and a 14% reduction in adult worms from pulmonary arteries were observed in rats immunized with FAGP compared to controls. However, there was a >50% reduction in rats immunized with MA or FA. The lengths of larvae and adults recovered from FAGP-immunized rats were shorter than those recovered from other groups. The number of first-stage larvae recovered from fecal material in FAGP-immunized rats was significantly reduced. Additionally, FAGP induced the highest splenocyte proliferation. Serum IgG titers were not directly correlated with protective immunity. An 84 kDa gut membrane protein was strongly recognized by anti-FAGP antibodies. Conclusion: We demonstrated that immune responses induced by FAGP reduced the growth, development, and reproduction of A. cantonensis in subsequent infections. While the possibility of using FAGP combining with MA or FA antigens as a multi-function vaccine in immune protection against A. cantonensis needs to be further elucidated, we hope that it provides a novel strategy for this parasite vaccine development. Keywords: Angiostrongylus cantonensis, Intestinal proteins, Protective immunity, Anti-fertilityhttp://www.sciencedirect.com/science/article/pii/S1684118217301482 |
| spellingShingle | Chien-Yu Lee Po-Ching Cheng Chen-Hsun Ho Chia-Kwung Fan David Chao Immunologic evaluation of extracted intestinal proteins from Angiostrongylus cantonensis adult worms Journal of Microbiology, Immunology and Infection |
| title | Immunologic evaluation of extracted intestinal proteins from Angiostrongylus cantonensis adult worms |
| title_full | Immunologic evaluation of extracted intestinal proteins from Angiostrongylus cantonensis adult worms |
| title_fullStr | Immunologic evaluation of extracted intestinal proteins from Angiostrongylus cantonensis adult worms |
| title_full_unstemmed | Immunologic evaluation of extracted intestinal proteins from Angiostrongylus cantonensis adult worms |
| title_short | Immunologic evaluation of extracted intestinal proteins from Angiostrongylus cantonensis adult worms |
| title_sort | immunologic evaluation of extracted intestinal proteins from angiostrongylus cantonensis adult worms |
| url | http://www.sciencedirect.com/science/article/pii/S1684118217301482 |
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