Background: Gastric cancer is one of the most common cancers and the second most common cause of cancer-related death worldwide. Identification of specific prognostic indicators might allow a better prognostic stratification and more effective therapy. Aim: To assess the expression and relationship...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2016-09-01
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| Series: | Journal of the Egyptian National Cancer Institute |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110036216300310 |
| _version_ | 1818851935390793728 |
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| author | Nesreen H. Hafez Neveen S. Tahoun |
| author_facet | Nesreen H. Hafez Neveen S. Tahoun |
| author_sort | Nesreen H. Hafez |
| collection | DOAJ |
| description | Background: Gastric cancer is one of the most common cancers and the second most common cause of cancer-related death worldwide. Identification of specific prognostic indicators might allow a better prognostic stratification and more effective therapy.
Aim: To assess the expression and relationship between COX-2 and VEGF protein in gastric adenocarcinoma and whether these markers are useful in predicting clinicopathological prognostic parameters.
Materials and methods: The study included 83 formalin-fixed paraffin embedded tissue samples of excised gastric adenocarcinoma and 20 non tumorous tissue controls. The slides were subjected to COX-2 and VEGF immunohistochemical staining using a streptavidin–biotinperoxidase according to the manufacturer’s protocol. The results were assessed independently by two pathologists. The relationships among COX-2 and VEGF expression and clinicopathological parameters were statistically analyzed.
Results: COX-2 and VEGF expressions were obviously higher in carcinoma tissues compared to normal mucosae (p < 0.001). The expression rate of COX-2 was 54.2% and of VEGF was 68.7%. COX-2 positive tumors were significantly correlated with Lauren classification, tumor depth and Helicobacter pylori infection (p < 0.001, p = 0.008, p = 0.035). VEGF was significantly associated with lymph node metastasis and tumor depth (p < 0.001). There was a positive association between VEGF and COX-2 expression in gastric adenocarcinoma (Kappa value = 0.55).
Conclusion: In gastric adenocarcinoma, COX-2 expression might serve as a powerful indicator for intestinal type carcinoma, locally advanced disease and H. pylori infection, while VEGF was related to loco-regional progression. COX-2 might be involved in the development of angiogenesis in gastric carcinoma through VEGF upregulation. |
| first_indexed | 2024-12-19T07:12:56Z |
| format | Article |
| id | doaj.art-c2a371e0fc514d4a901f6c903a851c68 |
| institution | Directory Open Access Journal |
| issn | 1110-0362 |
| language | English |
| last_indexed | 2024-12-19T07:12:56Z |
| publishDate | 2016-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of the Egyptian National Cancer Institute |
| spelling | doaj.art-c2a371e0fc514d4a901f6c903a851c682022-12-21T20:31:09ZengSpringerOpenJournal of the Egyptian National Cancer Institute1110-03622016-09-0128314915610.1016/j.jnci.2016.05.005Nesreen H. HafezNeveen S. TahounBackground: Gastric cancer is one of the most common cancers and the second most common cause of cancer-related death worldwide. Identification of specific prognostic indicators might allow a better prognostic stratification and more effective therapy. Aim: To assess the expression and relationship between COX-2 and VEGF protein in gastric adenocarcinoma and whether these markers are useful in predicting clinicopathological prognostic parameters. Materials and methods: The study included 83 formalin-fixed paraffin embedded tissue samples of excised gastric adenocarcinoma and 20 non tumorous tissue controls. The slides were subjected to COX-2 and VEGF immunohistochemical staining using a streptavidin–biotinperoxidase according to the manufacturer’s protocol. The results were assessed independently by two pathologists. The relationships among COX-2 and VEGF expression and clinicopathological parameters were statistically analyzed. Results: COX-2 and VEGF expressions were obviously higher in carcinoma tissues compared to normal mucosae (p < 0.001). The expression rate of COX-2 was 54.2% and of VEGF was 68.7%. COX-2 positive tumors were significantly correlated with Lauren classification, tumor depth and Helicobacter pylori infection (p < 0.001, p = 0.008, p = 0.035). VEGF was significantly associated with lymph node metastasis and tumor depth (p < 0.001). There was a positive association between VEGF and COX-2 expression in gastric adenocarcinoma (Kappa value = 0.55). Conclusion: In gastric adenocarcinoma, COX-2 expression might serve as a powerful indicator for intestinal type carcinoma, locally advanced disease and H. pylori infection, while VEGF was related to loco-regional progression. COX-2 might be involved in the development of angiogenesis in gastric carcinoma through VEGF upregulation.http://www.sciencedirect.com/science/article/pii/S1110036216300310Gastric adenocarcinomaCOX-2VEGFIHC |
| spellingShingle | Nesreen H. Hafez Neveen S. Tahoun Journal of the Egyptian National Cancer Institute Gastric adenocarcinoma COX-2 VEGF IHC |
| topic | Gastric adenocarcinoma COX-2 VEGF IHC |
| url | http://www.sciencedirect.com/science/article/pii/S1110036216300310 |