Development of microsatellite markers for the invasive mosquito Aedes koreicus
Abstract Background Aedes koreicus is a mosquito species native to East Asia which has recently invaded several countries in Europe. In Italy, this mosquito was first detected in the North-East in 2011 and is now widely distributed in the entire northern part of the country. The development of speci...
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-023-05823-z |
| _version_ | 1797453766095536128 |
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| author | Laura Soresinetti Irene Arnoldi Agata Negri Giovanni Naro Alice Michelutti Fabrizio Montarsi Andrea Mosca Claudio Bandi Paolo Gabrieli Sara Epis |
| author_facet | Laura Soresinetti Irene Arnoldi Agata Negri Giovanni Naro Alice Michelutti Fabrizio Montarsi Andrea Mosca Claudio Bandi Paolo Gabrieli Sara Epis |
| author_sort | Laura Soresinetti |
| collection | DOAJ |
| description | Abstract Background Aedes koreicus is a mosquito species native to East Asia which has recently invaded several countries in Europe. In Italy, this mosquito was first detected in the North-East in 2011 and is now widely distributed in the entire northern part of the country. The development of specific genetic markers, such as microsatellites, is necessary to uncover the dispersal routes of this mosquito from its native areas and, eventually, to plan future control interventions. Methods Available raw sequences of genomic DNA of Ae. koreicus were screened in silico using BLASTn to identify possible microsatellite-containing sequences. Specific primer pairs were then designed, and their efficiency was determined through polymerase chain reaction (PCR) on 32 individuals of Ae. koreicus collected in Italy. PCR conditions were optimised in three multiplex reactions. Genotyping of individual mosquitoes was performed on both single and multiplex PCR reactions. Finally, analysis of intra-population variation was performed to assess the level of polymorphism of the markers. Results Mosquito genotyping provided consistent results in both single and multiplex reactions. Out of the 31 microsatellite markers identified in the Ae. koreicus genome raw sequences, 11 were polymorphic in the examined mosquito samples. Conclusions The results show that the 11 microsatellite markers developed here hold potential for investigating the genetic structure of Ae. koreicus populations. These markers could thus represent a novel and useful tool to infer the routes of invasion of this mosquito species into Europe and other non-native areas. Graphical Abstract |
| first_indexed | 2024-03-09T15:27:36Z |
| format | Article |
| id | doaj.art-c2a4af6a626145139e22374030b6d2fe |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-09T15:27:36Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-c2a4af6a626145139e22374030b6d2fe2023-11-26T12:28:57ZengBMCParasites & Vectors1756-33052023-07-011611710.1186/s13071-023-05823-zDevelopment of microsatellite markers for the invasive mosquito Aedes koreicusLaura Soresinetti0Irene Arnoldi1Agata Negri2Giovanni Naro3Alice Michelutti4Fabrizio Montarsi5Andrea Mosca6Claudio Bandi7Paolo Gabrieli8Sara Epis9Department of Biosciences and Pediatric Clinical Research Center “Romeo Ed Enrica Invernizzi”, University of MilanDepartment of Biosciences and Pediatric Clinical Research Center “Romeo Ed Enrica Invernizzi”, University of MilanDepartment of Biosciences and Pediatric Clinical Research Center “Romeo Ed Enrica Invernizzi”, University of MilanDepartment of Biosciences and Pediatric Clinical Research Center “Romeo Ed Enrica Invernizzi”, University of MilanIstituto Zooprofilattico Sperimentale Delle VenezieIstituto Zooprofilattico Sperimentale Delle VenezieIstituto Per Le Piante da Legno E L ’Ambiente, I.P.L.A. S.P.ADepartment of Biosciences and Pediatric Clinical Research Center “Romeo Ed Enrica Invernizzi”, University of MilanDepartment of Biosciences and Pediatric Clinical Research Center “Romeo Ed Enrica Invernizzi”, University of MilanDepartment of Biosciences and Pediatric Clinical Research Center “Romeo Ed Enrica Invernizzi”, University of MilanAbstract Background Aedes koreicus is a mosquito species native to East Asia which has recently invaded several countries in Europe. In Italy, this mosquito was first detected in the North-East in 2011 and is now widely distributed in the entire northern part of the country. The development of specific genetic markers, such as microsatellites, is necessary to uncover the dispersal routes of this mosquito from its native areas and, eventually, to plan future control interventions. Methods Available raw sequences of genomic DNA of Ae. koreicus were screened in silico using BLASTn to identify possible microsatellite-containing sequences. Specific primer pairs were then designed, and their efficiency was determined through polymerase chain reaction (PCR) on 32 individuals of Ae. koreicus collected in Italy. PCR conditions were optimised in three multiplex reactions. Genotyping of individual mosquitoes was performed on both single and multiplex PCR reactions. Finally, analysis of intra-population variation was performed to assess the level of polymorphism of the markers. Results Mosquito genotyping provided consistent results in both single and multiplex reactions. Out of the 31 microsatellite markers identified in the Ae. koreicus genome raw sequences, 11 were polymorphic in the examined mosquito samples. Conclusions The results show that the 11 microsatellite markers developed here hold potential for investigating the genetic structure of Ae. koreicus populations. These markers could thus represent a novel and useful tool to infer the routes of invasion of this mosquito species into Europe and other non-native areas. Graphical Abstracthttps://doi.org/10.1186/s13071-023-05823-zInvasive mosquitoesAedes mosquitoesSimple sequence repeats (SSRs)Population geneticsMonitoring |
| spellingShingle | Laura Soresinetti Irene Arnoldi Agata Negri Giovanni Naro Alice Michelutti Fabrizio Montarsi Andrea Mosca Claudio Bandi Paolo Gabrieli Sara Epis Development of microsatellite markers for the invasive mosquito Aedes koreicus Parasites & Vectors Invasive mosquitoes Aedes mosquitoes Simple sequence repeats (SSRs) Population genetics Monitoring |
| title | Development of microsatellite markers for the invasive mosquito Aedes koreicus |
| title_full | Development of microsatellite markers for the invasive mosquito Aedes koreicus |
| title_fullStr | Development of microsatellite markers for the invasive mosquito Aedes koreicus |
| title_full_unstemmed | Development of microsatellite markers for the invasive mosquito Aedes koreicus |
| title_short | Development of microsatellite markers for the invasive mosquito Aedes koreicus |
| title_sort | development of microsatellite markers for the invasive mosquito aedes koreicus |
| topic | Invasive mosquitoes Aedes mosquitoes Simple sequence repeats (SSRs) Population genetics Monitoring |
| url | https://doi.org/10.1186/s13071-023-05823-z |
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