Effect of heat stress on growth performance, carcase characteristics, meat quality and rumen-muscle axis of Hu sheep
This study aimed to investigate the effects of heat stress (HS) on growth performance, carcase characteristics and rumen-muscle axis of Hu sheep and to explore the underlying molecular mechanisms of the actions by establishing a heat-stressed Hu sheep. The results showed that HS significantly decrea...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/1828051X.2023.2284886 |
| _version_ | 1797366087534247936 |
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| author | Sanbao Zhang Yu Zhang Yirong Wei Jianwei Zou Bao Yang Qian Wang Jun Lu Junzhi Lu Zihua Zheng Yanna Huang Qinyang Jiang |
| author_facet | Sanbao Zhang Yu Zhang Yirong Wei Jianwei Zou Bao Yang Qian Wang Jun Lu Junzhi Lu Zihua Zheng Yanna Huang Qinyang Jiang |
| author_sort | Sanbao Zhang |
| collection | DOAJ |
| description | This study aimed to investigate the effects of heat stress (HS) on growth performance, carcase characteristics and rumen-muscle axis of Hu sheep and to explore the underlying molecular mechanisms of the actions by establishing a heat-stressed Hu sheep. The results showed that HS significantly decreased growth performance and carcase characteristics, altered metabolic enzyme activities, and induced a shift in muscle energy metabolism towards a more glycolytic and less oxidative fibre type in the longissimus dorsi (LD) muscle (p < 0.05), thereby affecting the meat quality of Hu sheep. Furthermore, RNA-seq analysis of LD muscle found that HS down-regulated the expression of genes (ADIPOQ, ADRA2A, COL6A3, MYOC, PPARGC1A, etc.) involved in fat deposition and muscle development. 16S rDNA analyses of ruminal microbiome revealed that resist adverse environments-related Firmicutes, fatty acid synthesis-related Ruminococcaceae, Prevotellaceae, etc. were increased in the heat-stressed Hu sheep, and negatively correlated with genes involved in muscle development and lipid metabolism. However, energy metabolism-related Bacteroidetes and Tenericutes, and protein and carbohydrate cleavage-related Prevotella and Mollicutes were decreased in the heat-stressed Hu sheep and positively correlated with genes involved in muscle development and lipid metabolism. All together, these findings indicated that the rumen microbiota and gene expression profiles of muscle underwent responsive changes to HS, which impaired growth performance, carcase characteristics and meat quality in Hu sheep. Our findings provide valuable insights into the mechanisms underlying heat stress-induced effects in sheep, suggesting potential strategies for the prevention and control of HS as well as the development of new heat-resistant varieties in Hu sheep using molecular design breeding. |
| first_indexed | 2024-03-08T16:59:14Z |
| format | Article |
| id | doaj.art-c2a4b3e711b44c11aca3240fc1661078 |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-03-08T16:59:14Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-c2a4b3e711b44c11aca3240fc16610782024-01-04T15:59:08ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2024-12-012318710010.1080/1828051X.2023.22848862284886Effect of heat stress on growth performance, carcase characteristics, meat quality and rumen-muscle axis of Hu sheepSanbao Zhang0Yu Zhang1Yirong Wei2Jianwei Zou3Bao Yang4Qian Wang5Jun Lu6Junzhi Lu7Zihua Zheng8Yanna Huang9Qinyang Jiang10College of Animal Science and Technology, Guangxi UniversityCollege of Animal Science and Technology, Guangxi UniversityCollege of Animal Science and Technology, Guangxi UniversityCollege of Animal Science and Technology, Guangxi UniversityCollege of Animal Science and Technology, Guangxi UniversityCollege of Animal Science and Technology, Guangxi UniversityCollege of Animal Science and Technology, Guangxi UniversityInstitute for New Rural Development, Guangxi UniversityInstitute for New Rural Development, Guangxi UniversityCollege of Animal Science and Technology, Guangxi UniversityCollege of Animal Science and Technology, Guangxi UniversityThis study aimed to investigate the effects of heat stress (HS) on growth performance, carcase characteristics and rumen-muscle axis of Hu sheep and to explore the underlying molecular mechanisms of the actions by establishing a heat-stressed Hu sheep. The results showed that HS significantly decreased growth performance and carcase characteristics, altered metabolic enzyme activities, and induced a shift in muscle energy metabolism towards a more glycolytic and less oxidative fibre type in the longissimus dorsi (LD) muscle (p < 0.05), thereby affecting the meat quality of Hu sheep. Furthermore, RNA-seq analysis of LD muscle found that HS down-regulated the expression of genes (ADIPOQ, ADRA2A, COL6A3, MYOC, PPARGC1A, etc.) involved in fat deposition and muscle development. 16S rDNA analyses of ruminal microbiome revealed that resist adverse environments-related Firmicutes, fatty acid synthesis-related Ruminococcaceae, Prevotellaceae, etc. were increased in the heat-stressed Hu sheep, and negatively correlated with genes involved in muscle development and lipid metabolism. However, energy metabolism-related Bacteroidetes and Tenericutes, and protein and carbohydrate cleavage-related Prevotella and Mollicutes were decreased in the heat-stressed Hu sheep and positively correlated with genes involved in muscle development and lipid metabolism. All together, these findings indicated that the rumen microbiota and gene expression profiles of muscle underwent responsive changes to HS, which impaired growth performance, carcase characteristics and meat quality in Hu sheep. Our findings provide valuable insights into the mechanisms underlying heat stress-induced effects in sheep, suggesting potential strategies for the prevention and control of HS as well as the development of new heat-resistant varieties in Hu sheep using molecular design breeding.http://dx.doi.org/10.1080/1828051X.2023.2284886heat stressgrowth performancecarcase characteristics and meat qualityrumen-muscle axishu sheep |
| spellingShingle | Sanbao Zhang Yu Zhang Yirong Wei Jianwei Zou Bao Yang Qian Wang Jun Lu Junzhi Lu Zihua Zheng Yanna Huang Qinyang Jiang Effect of heat stress on growth performance, carcase characteristics, meat quality and rumen-muscle axis of Hu sheep Italian Journal of Animal Science heat stress growth performance carcase characteristics and meat quality rumen-muscle axis hu sheep |
| title | Effect of heat stress on growth performance, carcase characteristics, meat quality and rumen-muscle axis of Hu sheep |
| title_full | Effect of heat stress on growth performance, carcase characteristics, meat quality and rumen-muscle axis of Hu sheep |
| title_fullStr | Effect of heat stress on growth performance, carcase characteristics, meat quality and rumen-muscle axis of Hu sheep |
| title_full_unstemmed | Effect of heat stress on growth performance, carcase characteristics, meat quality and rumen-muscle axis of Hu sheep |
| title_short | Effect of heat stress on growth performance, carcase characteristics, meat quality and rumen-muscle axis of Hu sheep |
| title_sort | effect of heat stress on growth performance carcase characteristics meat quality and rumen muscle axis of hu sheep |
| topic | heat stress growth performance carcase characteristics and meat quality rumen-muscle axis hu sheep |
| url | http://dx.doi.org/10.1080/1828051X.2023.2284886 |
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