Characterization of Phosphate Membrane Transport in Saccharomyces cerevisiae CEN.PK113-5D under Low-Phosphate Conditions Using Aerobic Continuous Culture
Two different growth media, namely complex and defined media, were used to examine establishment of steady-state conditions in phosphate-limited culture system of Saccharomyces cerevisiae CEN.PK113-5D strain. Using the defined growth medium, it was possible to obtain steady state condition in the co...
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| Format: | Article |
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Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR
2005-03-01
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| Series: | Iranian Journal of Chemistry & Chemical Engineering |
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| Online Access: | http://www.ijcce.ac.ir/article_8131_47d18f7cdf6bd1d5c9f8819f9f1cc623.pdf |
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| author | Soheila Shokrollahzadeh Farzaneh Vahabzadeh Babak Bonakdarpour Mehri Sanati Bengt L Persson |
| author_facet | Soheila Shokrollahzadeh Farzaneh Vahabzadeh Babak Bonakdarpour Mehri Sanati Bengt L Persson |
| author_sort | Soheila Shokrollahzadeh |
| collection | DOAJ |
| description | Two different growth media, namely complex and defined media, were used to examine establishment of steady-state conditions in phosphate-limited culture system of Saccharomyces cerevisiae CEN.PK113-5D strain. Using the defined growth medium, it was possible to obtain steady state condition in the continuous culture. The effect of phosphate concentration on the growth of S. cerevisiae in phosphate-limited chemostat was studied at dilution rates between 0.08-0.45 h–1. The cells' growth followed Monod kinetics only over low dilution rates (0.08-0.22 h–1) in which the saturation constant (KS ) and maximum growth rate (μm ) were determined as 10 µM and 0.25 h–1, respectively. By increasing the dilution rates above 0.22 h–1, a significant change in the growth pattern was occurred, possibly due to intracellular accumulation of phosphate and/or extracellular accumulation of ethanol and also increased fermentative activity of the yeast cells. Phosphate transport of the yeast cells via plasma membrane transporters was kinetically characterized in a phosphate-limited chemostat culture. The rate of phosphate transport was measured using 32[P]-labeled orthophosphate in the concentration range of 0.4-2000 µM. High-affinity phosphate transport kinetics was observed over the entire range of dilution rates tested in this study. The corresponding Km values for phosphate were found to be in the range of 1.7 to 36 µM. Dilution rate of 0.22 h–1 showed biphasic pattern for phosphate uptake kinetics while the estimated Km values for this behavior were 1.7 and 284 µM. |
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| id | doaj.art-c2a627e2efe241e19c20222ad9388ca2 |
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| issn | 1021-9986 1021-9986 |
| language | English |
| last_indexed | 2024-04-14T04:12:05Z |
| publishDate | 2005-03-01 |
| publisher | Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR |
| record_format | Article |
| series | Iranian Journal of Chemistry & Chemical Engineering |
| spelling | doaj.art-c2a627e2efe241e19c20222ad9388ca22022-12-22T02:13:07ZengIranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECRIranian Journal of Chemistry & Chemical Engineering1021-99861021-99862005-03-0124141518131Characterization of Phosphate Membrane Transport in Saccharomyces cerevisiae CEN.PK113-5D under Low-Phosphate Conditions Using Aerobic Continuous CultureSoheila Shokrollahzadeh0Farzaneh Vahabzadeh1Babak Bonakdarpour2Mehri Sanati3Bengt L Persson4Food Engineering and Biotechnology Group, Department of Chemical Engineering, Amirkabir University of Technology, P.O.Box 15875-4413, Tehran, I.R. IRANFood Engineering and Biotechnology Group, Department of Chemical Engineering, Amirkabir University of Technology, P.O.Box 15875-4413, Tehran, I.R. IRANFood Engineering and Biotechnology Group, Department of Chemical Engineering, Amirkabir University of Technology, P.O.Box 15875-4413, Tehran, I.R. IRANSchool of Technology and Design, Växjö University, SwedenDepartment of Biochemistry and Biophysics, Wallenberg Laboratory, Stockholm University, SwedenTwo different growth media, namely complex and defined media, were used to examine establishment of steady-state conditions in phosphate-limited culture system of Saccharomyces cerevisiae CEN.PK113-5D strain. Using the defined growth medium, it was possible to obtain steady state condition in the continuous culture. The effect of phosphate concentration on the growth of S. cerevisiae in phosphate-limited chemostat was studied at dilution rates between 0.08-0.45 h–1. The cells' growth followed Monod kinetics only over low dilution rates (0.08-0.22 h–1) in which the saturation constant (KS ) and maximum growth rate (μm ) were determined as 10 µM and 0.25 h–1, respectively. By increasing the dilution rates above 0.22 h–1, a significant change in the growth pattern was occurred, possibly due to intracellular accumulation of phosphate and/or extracellular accumulation of ethanol and also increased fermentative activity of the yeast cells. Phosphate transport of the yeast cells via plasma membrane transporters was kinetically characterized in a phosphate-limited chemostat culture. The rate of phosphate transport was measured using 32[P]-labeled orthophosphate in the concentration range of 0.4-2000 µM. High-affinity phosphate transport kinetics was observed over the entire range of dilution rates tested in this study. The corresponding Km values for phosphate were found to be in the range of 1.7 to 36 µM. Dilution rate of 0.22 h–1 showed biphasic pattern for phosphate uptake kinetics while the estimated Km values for this behavior were 1.7 and 284 µM.http://www.ijcce.ac.ir/article_8131_47d18f7cdf6bd1d5c9f8819f9f1cc623.pdfcell membrane transportkinetics of phosphate transportsaccharomyces cerevisiaechemostat cultureyeast's growth kinetics |
| spellingShingle | Soheila Shokrollahzadeh Farzaneh Vahabzadeh Babak Bonakdarpour Mehri Sanati Bengt L Persson Characterization of Phosphate Membrane Transport in Saccharomyces cerevisiae CEN.PK113-5D under Low-Phosphate Conditions Using Aerobic Continuous Culture Iranian Journal of Chemistry & Chemical Engineering cell membrane transport kinetics of phosphate transport saccharomyces cerevisiae chemostat culture yeast's growth kinetics |
| title | Characterization of Phosphate Membrane Transport in Saccharomyces cerevisiae CEN.PK113-5D under Low-Phosphate Conditions Using Aerobic Continuous Culture |
| title_full | Characterization of Phosphate Membrane Transport in Saccharomyces cerevisiae CEN.PK113-5D under Low-Phosphate Conditions Using Aerobic Continuous Culture |
| title_fullStr | Characterization of Phosphate Membrane Transport in Saccharomyces cerevisiae CEN.PK113-5D under Low-Phosphate Conditions Using Aerobic Continuous Culture |
| title_full_unstemmed | Characterization of Phosphate Membrane Transport in Saccharomyces cerevisiae CEN.PK113-5D under Low-Phosphate Conditions Using Aerobic Continuous Culture |
| title_short | Characterization of Phosphate Membrane Transport in Saccharomyces cerevisiae CEN.PK113-5D under Low-Phosphate Conditions Using Aerobic Continuous Culture |
| title_sort | characterization of phosphate membrane transport in saccharomyces cerevisiae cen pk113 5d under low phosphate conditions using aerobic continuous culture |
| topic | cell membrane transport kinetics of phosphate transport saccharomyces cerevisiae chemostat culture yeast's growth kinetics |
| url | http://www.ijcce.ac.ir/article_8131_47d18f7cdf6bd1d5c9f8819f9f1cc623.pdf |
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