Optimized CRISPR-Cas9 Genome Editing for <italic toggle="yes">Leishmania</italic> and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms

Optimized CRISPR-Cas9 Genome Editing for <italic toggle="yes">Leishmania</italic> and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms

ABSTRACT CRISPR-Cas9-mediated genome editing has recently been adapted for Leishmania spp. parasites, the causative agents of human leishmaniasis. We have optimized this genome-editing tool by selecting for cells with CRISPR-Cas9 activity through cotargeting the miltefosine transporter gene; mutatio...

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Detalhes bibliográficos
Main Authors: Wen-Wei Zhang, Patrick Lypaczewski, Greg Matlashewski
Formato: Artigo
Idioma:English
Publicado em: American Society for Microbiology 2017-02-01
Colecção:mSphere
Assuntos:
CRISPR-Cas9
chromosomal translocation
co-CRISPR targeting
homology-directed repair
Leishmania
MMEJ
Acesso em linha:https://journals.asm.org/doi/10.1128/mSphere.00340-16

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