Genome-Wide Identification of Sucrose Transporter Genes and Functional Analysis of <i>RsSUC1b</i> in Radish (<i>Raphanus sativus</i> L.)
In most higher plants, sucrose is the significant form of carbohydrate for long-distance transportation. Sucrose transporters/sucrose carriers (SUTs/SUCs) are involved in the loading and unloading of sucrose in phloem and play an important role in the growth and development of plants. In this study,...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/8/11/1058 |
| _version_ | 1827646528521502720 |
|---|---|
| author | Xiaofeng Zhu Xiaoli Zhang Yang Cao Ruixian Xin Yinbo Ma Lun Wang Liang Xu Yan Wang Rui Liu Liwang Liu |
| author_facet | Xiaofeng Zhu Xiaoli Zhang Yang Cao Ruixian Xin Yinbo Ma Lun Wang Liang Xu Yan Wang Rui Liu Liwang Liu |
| author_sort | Xiaofeng Zhu |
| collection | DOAJ |
| description | In most higher plants, sucrose is the significant form of carbohydrate for long-distance transportation. Sucrose transporters/sucrose carriers (SUTs/SUCs) are involved in the loading and unloading of sucrose in phloem and play an important role in the growth and development of plants. In this study, 12 <i>RsSUC</i> genes were first identified from the radish genome, and their phylogenetic relationships, gene structure, and conserved motifs were further analyzed. RT-qPCR results indicated that <i>RsSUC</i> genes exhibited various expression patterns in different tissues and development stages of the radish. Overexpression of <i>RsSUC1b</i> in <i>Arabidopsis</i> significantly improved the uptake efficiency of exogenous sucrose, and promoted leaves and lateral root growth. In addition, the transgenic plants flowered significantly earlier than wild-type (WT) plants, and the soluble sugar contents (SSCs) including sucrose, glucose, and fructose in the mature leaves and pods were increased. It could be inferred that RsSUC1b is a plasma membrane sucrose transporter and plays a vital role in sucrose transportation and sugar accumulation during plant growth and development. These findings provided novel insights into the biological function of <i>RsSUC</i> genes and facilitate dissecting the molecular mechanism underlying sugar transport during radish development. |
| first_indexed | 2024-03-09T19:02:55Z |
| format | Article |
| id | doaj.art-c2a9f82b5d79488382e1e9179688f1fa |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T19:02:55Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-c2a9f82b5d79488382e1e9179688f1fa2023-11-24T04:53:31ZengMDPI AGHorticulturae2311-75242022-11-01811105810.3390/horticulturae8111058Genome-Wide Identification of Sucrose Transporter Genes and Functional Analysis of <i>RsSUC1b</i> in Radish (<i>Raphanus sativus</i> L.)Xiaofeng Zhu0Xiaoli Zhang1Yang Cao2Ruixian Xin3Yinbo Ma4Lun Wang5Liang Xu6Yan Wang7Rui Liu8Liwang Liu9National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China), Ministry of Agriculture and Rural Affairs of P.R.C., College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China), Ministry of Agriculture and Rural Affairs of P.R.C., College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China), Ministry of Agriculture and Rural Affairs of P.R.C., College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China), Ministry of Agriculture and Rural Affairs of P.R.C., College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China), Ministry of Agriculture and Rural Affairs of P.R.C., College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China), Ministry of Agriculture and Rural Affairs of P.R.C., College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China), Ministry of Agriculture and Rural Affairs of P.R.C., College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaNational Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China), Ministry of Agriculture and Rural Affairs of P.R.C., College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaIn most higher plants, sucrose is the significant form of carbohydrate for long-distance transportation. Sucrose transporters/sucrose carriers (SUTs/SUCs) are involved in the loading and unloading of sucrose in phloem and play an important role in the growth and development of plants. In this study, 12 <i>RsSUC</i> genes were first identified from the radish genome, and their phylogenetic relationships, gene structure, and conserved motifs were further analyzed. RT-qPCR results indicated that <i>RsSUC</i> genes exhibited various expression patterns in different tissues and development stages of the radish. Overexpression of <i>RsSUC1b</i> in <i>Arabidopsis</i> significantly improved the uptake efficiency of exogenous sucrose, and promoted leaves and lateral root growth. In addition, the transgenic plants flowered significantly earlier than wild-type (WT) plants, and the soluble sugar contents (SSCs) including sucrose, glucose, and fructose in the mature leaves and pods were increased. It could be inferred that RsSUC1b is a plasma membrane sucrose transporter and plays a vital role in sucrose transportation and sugar accumulation during plant growth and development. These findings provided novel insights into the biological function of <i>RsSUC</i> genes and facilitate dissecting the molecular mechanism underlying sugar transport during radish development.https://www.mdpi.com/2311-7524/8/11/1058radish<i>SUC</i> gene familyexpression analysis<i>RsSUC1b</i>functional analysis |
| spellingShingle | Xiaofeng Zhu Xiaoli Zhang Yang Cao Ruixian Xin Yinbo Ma Lun Wang Liang Xu Yan Wang Rui Liu Liwang Liu Genome-Wide Identification of Sucrose Transporter Genes and Functional Analysis of <i>RsSUC1b</i> in Radish (<i>Raphanus sativus</i> L.) Horticulturae radish <i>SUC</i> gene family expression analysis <i>RsSUC1b</i> functional analysis |
| title | Genome-Wide Identification of Sucrose Transporter Genes and Functional Analysis of <i>RsSUC1b</i> in Radish (<i>Raphanus sativus</i> L.) |
| title_full | Genome-Wide Identification of Sucrose Transporter Genes and Functional Analysis of <i>RsSUC1b</i> in Radish (<i>Raphanus sativus</i> L.) |
| title_fullStr | Genome-Wide Identification of Sucrose Transporter Genes and Functional Analysis of <i>RsSUC1b</i> in Radish (<i>Raphanus sativus</i> L.) |
| title_full_unstemmed | Genome-Wide Identification of Sucrose Transporter Genes and Functional Analysis of <i>RsSUC1b</i> in Radish (<i>Raphanus sativus</i> L.) |
| title_short | Genome-Wide Identification of Sucrose Transporter Genes and Functional Analysis of <i>RsSUC1b</i> in Radish (<i>Raphanus sativus</i> L.) |
| title_sort | genome wide identification of sucrose transporter genes and functional analysis of i rssuc1b i in radish i raphanus sativus i l |
| topic | radish <i>SUC</i> gene family expression analysis <i>RsSUC1b</i> functional analysis |
| url | https://www.mdpi.com/2311-7524/8/11/1058 |
| work_keys_str_mv | AT xiaofengzhu genomewideidentificationofsucrosetransportergenesandfunctionalanalysisofirssuc1biinradishiraphanussativusil AT xiaolizhang genomewideidentificationofsucrosetransportergenesandfunctionalanalysisofirssuc1biinradishiraphanussativusil AT yangcao genomewideidentificationofsucrosetransportergenesandfunctionalanalysisofirssuc1biinradishiraphanussativusil AT ruixianxin genomewideidentificationofsucrosetransportergenesandfunctionalanalysisofirssuc1biinradishiraphanussativusil AT yinboma genomewideidentificationofsucrosetransportergenesandfunctionalanalysisofirssuc1biinradishiraphanussativusil AT lunwang genomewideidentificationofsucrosetransportergenesandfunctionalanalysisofirssuc1biinradishiraphanussativusil AT liangxu genomewideidentificationofsucrosetransportergenesandfunctionalanalysisofirssuc1biinradishiraphanussativusil AT yanwang genomewideidentificationofsucrosetransportergenesandfunctionalanalysisofirssuc1biinradishiraphanussativusil AT ruiliu genomewideidentificationofsucrosetransportergenesandfunctionalanalysisofirssuc1biinradishiraphanussativusil AT liwangliu genomewideidentificationofsucrosetransportergenesandfunctionalanalysisofirssuc1biinradishiraphanussativusil |