The protective effects of peptides from Chinese baijiu on AAPH-induced oxidative stress in HepG2 cells via Nrf2-mediated signaling pathway
Antioxidant peptides have been widely reported. However, only a few reports have been published examining the antioxidant peptides derived from Chinese baijiu. In this study, 6 novel peptides derived from Chinese baijiu were identified successfully using high-performance liquid chromatography-quadru...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2022-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022000933 |
| _version_ | 1797719527367114752 |
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| author | Jiaying Huo Yuezhang Ming Huifeng Li Anjun Li Jiwen Zhao Mingquan Huang Weizheng Sun Jihong Wu Jinglin Zhang |
| author_facet | Jiaying Huo Yuezhang Ming Huifeng Li Anjun Li Jiwen Zhao Mingquan Huang Weizheng Sun Jihong Wu Jinglin Zhang |
| author_sort | Jiaying Huo |
| collection | DOAJ |
| description | Antioxidant peptides have been widely reported. However, only a few reports have been published examining the antioxidant peptides derived from Chinese baijiu. In this study, 6 novel peptides derived from Chinese baijiu were identified successfully using high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (HPLC-QTOF-MS) with a concentration of 0.835–24.540 µg/L. The underlying molecular mechanisms were investigated, and their cytoprotective effects were examined against 2,2’-azobis (2-methylpropanimidamidine) dihydrochloride (AAPH)-induced oxidative stress in HepG2 cells. The results showed that these peptides exerted protective effects by suppressing reactive oxygen species (ROS) generation, preventing malondialdehyde (MDA) formation, and upregulating cellular antioxidant enzyme activities (SOD, CAT, and GSH-Px) in a dose-dependent manner. Further experiments proved that these peptides exerted antioxidant effects via Nrf2/ARE-mediated signaling pathway by promoting Nrf2 nuclear translocation, inhibiting ubiquitination, and enhancing transcription capacity of Nrf2 in HepG2 cells. These findings provide the molecular basis for the effects of antioxidant peptides derived from Chinese baijiu, which is important for a deeper understanding of the relationship between human health and moderate drinking. |
| first_indexed | 2024-03-12T09:07:14Z |
| format | Article |
| id | doaj.art-c2abf27b2cbb4104a5b241394d055251 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T09:07:14Z |
| publishDate | 2022-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-c2abf27b2cbb4104a5b241394d0552512023-09-02T15:16:05ZengTsinghua University PressFood Science and Human Wellness2213-45302022-11-0111615271538The protective effects of peptides from Chinese baijiu on AAPH-induced oxidative stress in HepG2 cells via Nrf2-mediated signaling pathwayJiaying Huo0Yuezhang Ming1Huifeng Li2Anjun Li3Jiwen Zhao4Mingquan Huang5Weizheng Sun6Jihong Wu7Jinglin Zhang8Key Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing 100048, China; School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaInner Mongolia Taibusiqi Grassland King Wine Co., Ltd., Inner Mongolia 027000, ChinaInner Mongolia Taibusiqi Grassland King Wine Co., Ltd., Inner Mongolia 027000, ChinaAnhui Gujing Distillery Co., Ltd., Anhui 236000, ChinaShandong Bandaojing Co., Ltd., Shandong 256300, ChinaKey Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing 100048, China; Corresponding authors.School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaKey Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing 100048, China; Corresponding authors.Key Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing 100048, ChinaAntioxidant peptides have been widely reported. However, only a few reports have been published examining the antioxidant peptides derived from Chinese baijiu. In this study, 6 novel peptides derived from Chinese baijiu were identified successfully using high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (HPLC-QTOF-MS) with a concentration of 0.835–24.540 µg/L. The underlying molecular mechanisms were investigated, and their cytoprotective effects were examined against 2,2’-azobis (2-methylpropanimidamidine) dihydrochloride (AAPH)-induced oxidative stress in HepG2 cells. The results showed that these peptides exerted protective effects by suppressing reactive oxygen species (ROS) generation, preventing malondialdehyde (MDA) formation, and upregulating cellular antioxidant enzyme activities (SOD, CAT, and GSH-Px) in a dose-dependent manner. Further experiments proved that these peptides exerted antioxidant effects via Nrf2/ARE-mediated signaling pathway by promoting Nrf2 nuclear translocation, inhibiting ubiquitination, and enhancing transcription capacity of Nrf2 in HepG2 cells. These findings provide the molecular basis for the effects of antioxidant peptides derived from Chinese baijiu, which is important for a deeper understanding of the relationship between human health and moderate drinking.http://www.sciencedirect.com/science/article/pii/S2213453022000933Chinese baijiuHigh-performance liquid chromatography-quadrupole-time-of-flight mass spectrometryAntioxidant peptidesHepG2 cellsNrf2 signaling pathway |
| spellingShingle | Jiaying Huo Yuezhang Ming Huifeng Li Anjun Li Jiwen Zhao Mingquan Huang Weizheng Sun Jihong Wu Jinglin Zhang The protective effects of peptides from Chinese baijiu on AAPH-induced oxidative stress in HepG2 cells via Nrf2-mediated signaling pathway Food Science and Human Wellness Chinese baijiu High-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry Antioxidant peptides HepG2 cells Nrf2 signaling pathway |
| title | The protective effects of peptides from Chinese baijiu on AAPH-induced oxidative stress in HepG2 cells via Nrf2-mediated signaling pathway |
| title_full | The protective effects of peptides from Chinese baijiu on AAPH-induced oxidative stress in HepG2 cells via Nrf2-mediated signaling pathway |
| title_fullStr | The protective effects of peptides from Chinese baijiu on AAPH-induced oxidative stress in HepG2 cells via Nrf2-mediated signaling pathway |
| title_full_unstemmed | The protective effects of peptides from Chinese baijiu on AAPH-induced oxidative stress in HepG2 cells via Nrf2-mediated signaling pathway |
| title_short | The protective effects of peptides from Chinese baijiu on AAPH-induced oxidative stress in HepG2 cells via Nrf2-mediated signaling pathway |
| title_sort | protective effects of peptides from chinese baijiu on aaph induced oxidative stress in hepg2 cells via nrf2 mediated signaling pathway |
| topic | Chinese baijiu High-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry Antioxidant peptides HepG2 cells Nrf2 signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022000933 |
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