Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach
Abstract Background Cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. The aim of this study was to investigate the presence of Cyclospora in captive non-human primates. Methods A total of 119 faecal samples from Pan troglodytes, Macaca sylvanus,...
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| Format: | Article |
| Language: | English |
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BMC
2015-05-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-015-0872-8 |
| _version_ | 1797811843111059456 |
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| author | Marianna Marangi Anson V Koehler Sergio A Zanzani Maria T Manfredi Emanuele Brianti Annunziata Giangaspero Robin B Gasser |
| author_facet | Marianna Marangi Anson V Koehler Sergio A Zanzani Maria T Manfredi Emanuele Brianti Annunziata Giangaspero Robin B Gasser |
| author_sort | Marianna Marangi |
| collection | DOAJ |
| description | Abstract Background Cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. The aim of this study was to investigate the presence of Cyclospora in captive non-human primates. Methods A total of 119 faecal samples from Pan troglodytes, Macaca sylvanus, Cercopithecus cephus, Erythrocebus patas, Chlorocebus aethiops and Macaca fascicularis from a wildlife animal rescue center as well as from Macaca fascicularis from an experimental primate research center were tested for the presence of Cyclospora by quantitative real-time PCR (qPCR) and single-strand conformation polymorphism (SSCP) analysis. Results Cyclospora was detected in three Pan troglodytes (13.6%) and nine (9.3%) Macaca fascicularis. Conclusions The present study represents the first record of Cyclospora in captive primates in Europe, suggesting the presence of Cyclospora cayetanensis, which is transmissible to humans. |
| first_indexed | 2024-03-13T07:29:47Z |
| format | Article |
| id | doaj.art-c2add3bba2744100b00a6ba36231fdfc |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:29:47Z |
| publishDate | 2015-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-c2add3bba2744100b00a6ba36231fdfc2023-06-04T11:11:09ZengBMCParasites & Vectors1756-33052015-05-01811510.1186/s13071-015-0872-8Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approachMarianna Marangi0Anson V Koehler1Sergio A Zanzani2Maria T Manfredi3Emanuele Brianti4Annunziata Giangaspero5Robin B Gasser6Department of Science of Agriculture, Food and Environment, University of FoggiaFaculty of Veterinary and Agricultural Sciences, The University of MelbourneDepartment of Animal Pathology, Hygiene and Public Health, University of MilanDepartment of Animal Pathology, Hygiene and Public Health, University of MilanDepartment of Veterinary Sciences, University of MessinaDepartment of Science of Agriculture, Food and Environment, University of FoggiaFaculty of Veterinary and Agricultural Sciences, The University of MelbourneAbstract Background Cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. The aim of this study was to investigate the presence of Cyclospora in captive non-human primates. Methods A total of 119 faecal samples from Pan troglodytes, Macaca sylvanus, Cercopithecus cephus, Erythrocebus patas, Chlorocebus aethiops and Macaca fascicularis from a wildlife animal rescue center as well as from Macaca fascicularis from an experimental primate research center were tested for the presence of Cyclospora by quantitative real-time PCR (qPCR) and single-strand conformation polymorphism (SSCP) analysis. Results Cyclospora was detected in three Pan troglodytes (13.6%) and nine (9.3%) Macaca fascicularis. Conclusions The present study represents the first record of Cyclospora in captive primates in Europe, suggesting the presence of Cyclospora cayetanensis, which is transmissible to humans.https://doi.org/10.1186/s13071-015-0872-8CyclosporaqPCRMutation scanning-based sequencingNon-human primatesZoonosis |
| spellingShingle | Marianna Marangi Anson V Koehler Sergio A Zanzani Maria T Manfredi Emanuele Brianti Annunziata Giangaspero Robin B Gasser Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach Parasites & Vectors Cyclospora qPCR Mutation scanning-based sequencing Non-human primates Zoonosis |
| title | Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach |
| title_full | Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach |
| title_fullStr | Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach |
| title_full_unstemmed | Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach |
| title_short | Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach |
| title_sort | detection of cyclospora in captive chimpanzees and macaques by a quantitative pcr based mutation scanning approach |
| topic | Cyclospora qPCR Mutation scanning-based sequencing Non-human primates Zoonosis |
| url | https://doi.org/10.1186/s13071-015-0872-8 |
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