Growth in Hyper-Concentrated Sweet Whey Triggers Multi Stress Tolerance and Spray Drying Survival in Lactobacillus casei BL23: From the Molecular Basis to New Perspectives for Sustainable Probiotic Production
Lactobacillus casei BL23 has a recognized probiotic potential, which includes immune modulation, protection toward induced colitis, toward induced colon cancer and toward dissemination of pathogens. In L. casei, as well as in other probiotics, both probiotic and technological abilities are highly de...
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| Format: | Article |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02548/full |
| _version_ | 1819228149086420992 |
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| author | Song Huang Song Huang Floriane Gaucher Floriane Gaucher Chantal Cauty Julien Jardin Yves Le Loir Romain Jeantet Romain Jeantet Xiao Dong Chen Gwénaël Jan |
| author_facet | Song Huang Song Huang Floriane Gaucher Floriane Gaucher Chantal Cauty Julien Jardin Yves Le Loir Romain Jeantet Romain Jeantet Xiao Dong Chen Gwénaël Jan |
| author_sort | Song Huang |
| collection | DOAJ |
| description | Lactobacillus casei BL23 has a recognized probiotic potential, which includes immune modulation, protection toward induced colitis, toward induced colon cancer and toward dissemination of pathogens. In L. casei, as well as in other probiotics, both probiotic and technological abilities are highly dependent (1) on the substrate used to grow bacteria and (2) on the process used to dry and store this biomass. Production and storage of probiotics, at a reasonable financial and environmental cost, becomes a crucial challenge. Food-grade media must be used, and minimal process is preferred. In this context, we have developed a “2-in-1” medium used both to grow and to dry L. casei BL23, considered a fragile probiotic strain. This medium consists in hyper-concentrated sweet whey (HCSW). L. casei BL23 grows in HCSW up to 30% dry matter, which is 6 times-concentrated sweet whey. Compared to isotonic sweet whey (5% dry matter), these growth conditions enhanced tolerance of L. casei BL23 toward heat, acid and bile salts stress. HCSW also triggered intracellular accumulation of polyphosphate, of glycogen and of trehalose. A gel-free global proteomic differential analysis further evidenced overexpression of proteins involved in pathways known to participate in stress adaptation, including environmental signal transduction, oxidative and metal defense, DNA repair, protein turnover and repair, carbohydrate, phosphate and amino acid metabolism, and in osmoadaptation. Accordingly, HCSW cultures of L. casei BL23 exhibited enhanced survival upon spray drying, a process known to drastically affect bacterial viability. This work opens new perspectives for sustainable production of dried probiotic lactobacilli, using food industry by-products and lowering energy costs. |
| first_indexed | 2024-12-23T10:52:41Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T10:52:41Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c2aded0042ad42c2a43a17a5ea8c39a52022-12-21T17:49:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-10-01910.3389/fmicb.2018.02548387519Growth in Hyper-Concentrated Sweet Whey Triggers Multi Stress Tolerance and Spray Drying Survival in Lactobacillus casei BL23: From the Molecular Basis to New Perspectives for Sustainable Probiotic ProductionSong Huang0Song Huang1Floriane Gaucher2Floriane Gaucher3Chantal Cauty4Julien Jardin5Yves Le Loir6Romain Jeantet7Romain Jeantet8Xiao Dong Chen9Gwénaël Jan10Suzhou Key Laboratory of Green Chemical Engineering, School of Chemical and Environmental Engineering, College of Chemistry, Chemical Engineering and Material Science, Soochow University, Jiangsu, ChinaUMR1253 STLO, Agrocampus Ouest, INRA, Rennes, FranceUMR1253 STLO, Agrocampus Ouest, INRA, Rennes, FranceBioprox, Levallois-Perret, FranceUMR1253 STLO, Agrocampus Ouest, INRA, Rennes, FranceUMR1253 STLO, Agrocampus Ouest, INRA, Rennes, FranceUMR1253 STLO, Agrocampus Ouest, INRA, Rennes, FranceSuzhou Key Laboratory of Green Chemical Engineering, School of Chemical and Environmental Engineering, College of Chemistry, Chemical Engineering and Material Science, Soochow University, Jiangsu, ChinaUMR1253 STLO, Agrocampus Ouest, INRA, Rennes, FranceSuzhou Key Laboratory of Green Chemical Engineering, School of Chemical and Environmental Engineering, College of Chemistry, Chemical Engineering and Material Science, Soochow University, Jiangsu, ChinaUMR1253 STLO, Agrocampus Ouest, INRA, Rennes, FranceLactobacillus casei BL23 has a recognized probiotic potential, which includes immune modulation, protection toward induced colitis, toward induced colon cancer and toward dissemination of pathogens. In L. casei, as well as in other probiotics, both probiotic and technological abilities are highly dependent (1) on the substrate used to grow bacteria and (2) on the process used to dry and store this biomass. Production and storage of probiotics, at a reasonable financial and environmental cost, becomes a crucial challenge. Food-grade media must be used, and minimal process is preferred. In this context, we have developed a “2-in-1” medium used both to grow and to dry L. casei BL23, considered a fragile probiotic strain. This medium consists in hyper-concentrated sweet whey (HCSW). L. casei BL23 grows in HCSW up to 30% dry matter, which is 6 times-concentrated sweet whey. Compared to isotonic sweet whey (5% dry matter), these growth conditions enhanced tolerance of L. casei BL23 toward heat, acid and bile salts stress. HCSW also triggered intracellular accumulation of polyphosphate, of glycogen and of trehalose. A gel-free global proteomic differential analysis further evidenced overexpression of proteins involved in pathways known to participate in stress adaptation, including environmental signal transduction, oxidative and metal defense, DNA repair, protein turnover and repair, carbohydrate, phosphate and amino acid metabolism, and in osmoadaptation. Accordingly, HCSW cultures of L. casei BL23 exhibited enhanced survival upon spray drying, a process known to drastically affect bacterial viability. This work opens new perspectives for sustainable production of dried probiotic lactobacilli, using food industry by-products and lowering energy costs.https://www.frontiersin.org/article/10.3389/fmicb.2018.02548/fullosmoregulationprobioticslactic acid bacteriastress responsephysiologylabel-free proteomics |
| spellingShingle | Song Huang Song Huang Floriane Gaucher Floriane Gaucher Chantal Cauty Julien Jardin Yves Le Loir Romain Jeantet Romain Jeantet Xiao Dong Chen Gwénaël Jan Growth in Hyper-Concentrated Sweet Whey Triggers Multi Stress Tolerance and Spray Drying Survival in Lactobacillus casei BL23: From the Molecular Basis to New Perspectives for Sustainable Probiotic Production Frontiers in Microbiology osmoregulation probiotics lactic acid bacteria stress response physiology label-free proteomics |
| title | Growth in Hyper-Concentrated Sweet Whey Triggers Multi Stress Tolerance and Spray Drying Survival in Lactobacillus casei BL23: From the Molecular Basis to New Perspectives for Sustainable Probiotic Production |
| title_full | Growth in Hyper-Concentrated Sweet Whey Triggers Multi Stress Tolerance and Spray Drying Survival in Lactobacillus casei BL23: From the Molecular Basis to New Perspectives for Sustainable Probiotic Production |
| title_fullStr | Growth in Hyper-Concentrated Sweet Whey Triggers Multi Stress Tolerance and Spray Drying Survival in Lactobacillus casei BL23: From the Molecular Basis to New Perspectives for Sustainable Probiotic Production |
| title_full_unstemmed | Growth in Hyper-Concentrated Sweet Whey Triggers Multi Stress Tolerance and Spray Drying Survival in Lactobacillus casei BL23: From the Molecular Basis to New Perspectives for Sustainable Probiotic Production |
| title_short | Growth in Hyper-Concentrated Sweet Whey Triggers Multi Stress Tolerance and Spray Drying Survival in Lactobacillus casei BL23: From the Molecular Basis to New Perspectives for Sustainable Probiotic Production |
| title_sort | growth in hyper concentrated sweet whey triggers multi stress tolerance and spray drying survival in lactobacillus casei bl23 from the molecular basis to new perspectives for sustainable probiotic production |
| topic | osmoregulation probiotics lactic acid bacteria stress response physiology label-free proteomics |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02548/full |
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