Pan-cancer analysis of IFN-γ with possible immunotherapeutic significance: a verification of single-cell sequencing and bulk omics research
BackgroundInterferon-gamma (IFN-γ), commonly referred to as type II interferon, is a crucial cytokine that coordinates the tumor immune process and has received considerable attention in tumor immunotherapy research. Previous studies have discussed the role and mechanisms associated with IFN-γ in sp...
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1202150/full |
| _version_ | 1797743402359455744 |
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| author | Xiaoying Wei Hanyi Ruan Yan Zhang Tianyu Qin Yujie Zhang Yan Qin Wei Li |
| author_facet | Xiaoying Wei Hanyi Ruan Yan Zhang Tianyu Qin Yujie Zhang Yan Qin Wei Li |
| author_sort | Xiaoying Wei |
| collection | DOAJ |
| description | BackgroundInterferon-gamma (IFN-γ), commonly referred to as type II interferon, is a crucial cytokine that coordinates the tumor immune process and has received considerable attention in tumor immunotherapy research. Previous studies have discussed the role and mechanisms associated with IFN-γ in specific tumors or diseases, but the relevant role of IFN-γ in pan-cancer remains uncertain.MethodsTCGA and GTEx RNA expression data and clinical data were downloaded. Additionally, we analyzed the role of IFN-γ on tumors by using a bioinformatic approach, which included the analysis of the correlation between IFN-γ in different tumors and expression, prognosis, functional status, TMB, MSI, immune cell infiltration, and TIDE. We also developed a PPI network for topological analysis of the network, identifying hub genes as those having a degree greater than IFN-γ levels.ResultIFN-γ was differentially expressed and predicted different survival statuses in a majority of tumor types in TCGA. Additionally, IFN-γ expression was strongly linked to factors like infiltration of T cells, immune checkpoints, immune-activating genes, immunosuppressive genes, chemokines, and chemokine receptors, as well as tumor purity, functional statuses, and prognostic value. Also, prognosis, CNV, and treatment response were all substantially correlated with IFN-γ-related gene expression. Particularly, the IFN-γ-related gene STAT1 exhibited the greatest percentage of SNVs and the largest percentage of SNPs in UCEC. Elevated expression levels of IFN-γ-related genes were found in a wide variety of tumor types, and this was shown to be positively linked to drug sensitivity for 20 different types of drugs.ConclusionIFN-γ is a good indicator of response to tumor immunotherapy and is likely to limit tumor progression, offering a novel approach for immunotherapy’s future development. |
| first_indexed | 2024-03-12T14:54:59Z |
| format | Article |
| id | doaj.art-c2af13341e54482d9a3e5f0ddc2fb683 |
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| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-12T14:54:59Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-c2af13341e54482d9a3e5f0ddc2fb6832023-08-15T04:13:29ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-08-011410.3389/fimmu.2023.12021501202150Pan-cancer analysis of IFN-γ with possible immunotherapeutic significance: a verification of single-cell sequencing and bulk omics researchXiaoying Wei0Hanyi Ruan1Yan Zhang2Tianyu Qin3Yujie Zhang4Yan Qin5Wei Li6Department of Health Management, The People’s Hospital of Guangxi Zhuang Autonomous Region and Research Center of Health Management, Guangxi Academy of Medical Sciences, Nanning, ChinaDepartment of Oncology, Guangxi Medical University Cancer Hospital, Nanning, ChinaDepartment of Oncology, Guangxi Medical University Cancer Hospital, Nanning, ChinaDepartment of Oncology, Guangxi Medical University Cancer Hospital, Nanning, ChinaDepartment of Oncology, Guangxi Medical University Cancer Hospital, Nanning, ChinaDepartment of Health Management, The People’s Hospital of Guangxi Zhuang Autonomous Region and Research Center of Health Management, Guangxi Academy of Medical Sciences, Nanning, ChinaDepartment of Health Management, The People’s Hospital of Guangxi Zhuang Autonomous Region and Research Center of Health Management, Guangxi Academy of Medical Sciences, Nanning, ChinaBackgroundInterferon-gamma (IFN-γ), commonly referred to as type II interferon, is a crucial cytokine that coordinates the tumor immune process and has received considerable attention in tumor immunotherapy research. Previous studies have discussed the role and mechanisms associated with IFN-γ in specific tumors or diseases, but the relevant role of IFN-γ in pan-cancer remains uncertain.MethodsTCGA and GTEx RNA expression data and clinical data were downloaded. Additionally, we analyzed the role of IFN-γ on tumors by using a bioinformatic approach, which included the analysis of the correlation between IFN-γ in different tumors and expression, prognosis, functional status, TMB, MSI, immune cell infiltration, and TIDE. We also developed a PPI network for topological analysis of the network, identifying hub genes as those having a degree greater than IFN-γ levels.ResultIFN-γ was differentially expressed and predicted different survival statuses in a majority of tumor types in TCGA. Additionally, IFN-γ expression was strongly linked to factors like infiltration of T cells, immune checkpoints, immune-activating genes, immunosuppressive genes, chemokines, and chemokine receptors, as well as tumor purity, functional statuses, and prognostic value. Also, prognosis, CNV, and treatment response were all substantially correlated with IFN-γ-related gene expression. Particularly, the IFN-γ-related gene STAT1 exhibited the greatest percentage of SNVs and the largest percentage of SNPs in UCEC. Elevated expression levels of IFN-γ-related genes were found in a wide variety of tumor types, and this was shown to be positively linked to drug sensitivity for 20 different types of drugs.ConclusionIFN-γ is a good indicator of response to tumor immunotherapy and is likely to limit tumor progression, offering a novel approach for immunotherapy’s future development.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1202150/fullIFN-γtumor microenvironmentimmunotherapypan-cancersingle-cell transcriptome sequencing |
| spellingShingle | Xiaoying Wei Hanyi Ruan Yan Zhang Tianyu Qin Yujie Zhang Yan Qin Wei Li Pan-cancer analysis of IFN-γ with possible immunotherapeutic significance: a verification of single-cell sequencing and bulk omics research Frontiers in Immunology IFN-γ tumor microenvironment immunotherapy pan-cancer single-cell transcriptome sequencing |
| title | Pan-cancer analysis of IFN-γ with possible immunotherapeutic significance: a verification of single-cell sequencing and bulk omics research |
| title_full | Pan-cancer analysis of IFN-γ with possible immunotherapeutic significance: a verification of single-cell sequencing and bulk omics research |
| title_fullStr | Pan-cancer analysis of IFN-γ with possible immunotherapeutic significance: a verification of single-cell sequencing and bulk omics research |
| title_full_unstemmed | Pan-cancer analysis of IFN-γ with possible immunotherapeutic significance: a verification of single-cell sequencing and bulk omics research |
| title_short | Pan-cancer analysis of IFN-γ with possible immunotherapeutic significance: a verification of single-cell sequencing and bulk omics research |
| title_sort | pan cancer analysis of ifn γ with possible immunotherapeutic significance a verification of single cell sequencing and bulk omics research |
| topic | IFN-γ tumor microenvironment immunotherapy pan-cancer single-cell transcriptome sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1202150/full |
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