Effect of heat stress on blood-brain barrier integrity in iPS cell-derived microvascular endothelial cell models.
The incidence of heatstroke has been increasing. Heatstroke has been shown to affect physiological barrier functions. However, there are few studies of the effect of heat stress on the blood-brain barrier (BBB) function. In this study, we investigated the influence of heat stress on brain microvascu...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0222113 |
| _version_ | 1819259605853667328 |
|---|---|
| author | Tomoko Yamaguchi Kentaro Shimizu Yasuhiro Kokubu Misae Nishijima Shuko Takeda Hiroshi Ogura Kenji Kawabata |
| author_facet | Tomoko Yamaguchi Kentaro Shimizu Yasuhiro Kokubu Misae Nishijima Shuko Takeda Hiroshi Ogura Kenji Kawabata |
| author_sort | Tomoko Yamaguchi |
| collection | DOAJ |
| description | The incidence of heatstroke has been increasing. Heatstroke has been shown to affect physiological barrier functions. However, there are few studies of the effect of heat stress on the blood-brain barrier (BBB) function. In this study, we investigated the influence of heat stress on brain microvascular endothelial cells in vivo and in vitro. Heatstroke model mice administered Texas Red-dextran showed leakage outside the brain vessel walls. In addition, trans-endothelial electrical resistance (TEER) value was significantly reduced in induced pluripotent stem (iPS) cell-derived brain microvascular endothelial cells under heat stress by reducing claudin-5 expression. In addition, our results showed that the expression level of P-glycoprotein (P-gp) was increased in iPS cell-derived brain microvascular endothelial cells under heat stress. Furthermore, serum from heatstroke model mice could impair the BBB integrity of iPS cell-derived brain microvascular endothelial cells. These results suggest that BBB integrity was affected by heat stress in vivo and in vitro and provide important insights into the development of new therapeutic strategies for heatstroke patients. |
| first_indexed | 2024-12-23T19:12:40Z |
| format | Article |
| id | doaj.art-c2afb4bc78b943ef8102ad2e2c70a47f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T19:12:40Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c2afb4bc78b943ef8102ad2e2c70a47f2022-12-21T17:34:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01149e022211310.1371/journal.pone.0222113Effect of heat stress on blood-brain barrier integrity in iPS cell-derived microvascular endothelial cell models.Tomoko YamaguchiKentaro ShimizuYasuhiro KokubuMisae NishijimaShuko TakedaHiroshi OguraKenji KawabataThe incidence of heatstroke has been increasing. Heatstroke has been shown to affect physiological barrier functions. However, there are few studies of the effect of heat stress on the blood-brain barrier (BBB) function. In this study, we investigated the influence of heat stress on brain microvascular endothelial cells in vivo and in vitro. Heatstroke model mice administered Texas Red-dextran showed leakage outside the brain vessel walls. In addition, trans-endothelial electrical resistance (TEER) value was significantly reduced in induced pluripotent stem (iPS) cell-derived brain microvascular endothelial cells under heat stress by reducing claudin-5 expression. In addition, our results showed that the expression level of P-glycoprotein (P-gp) was increased in iPS cell-derived brain microvascular endothelial cells under heat stress. Furthermore, serum from heatstroke model mice could impair the BBB integrity of iPS cell-derived brain microvascular endothelial cells. These results suggest that BBB integrity was affected by heat stress in vivo and in vitro and provide important insights into the development of new therapeutic strategies for heatstroke patients.https://doi.org/10.1371/journal.pone.0222113 |
| spellingShingle | Tomoko Yamaguchi Kentaro Shimizu Yasuhiro Kokubu Misae Nishijima Shuko Takeda Hiroshi Ogura Kenji Kawabata Effect of heat stress on blood-brain barrier integrity in iPS cell-derived microvascular endothelial cell models. PLoS ONE |
| title | Effect of heat stress on blood-brain barrier integrity in iPS cell-derived microvascular endothelial cell models. |
| title_full | Effect of heat stress on blood-brain barrier integrity in iPS cell-derived microvascular endothelial cell models. |
| title_fullStr | Effect of heat stress on blood-brain barrier integrity in iPS cell-derived microvascular endothelial cell models. |
| title_full_unstemmed | Effect of heat stress on blood-brain barrier integrity in iPS cell-derived microvascular endothelial cell models. |
| title_short | Effect of heat stress on blood-brain barrier integrity in iPS cell-derived microvascular endothelial cell models. |
| title_sort | effect of heat stress on blood brain barrier integrity in ips cell derived microvascular endothelial cell models |
| url | https://doi.org/10.1371/journal.pone.0222113 |
| work_keys_str_mv | AT tomokoyamaguchi effectofheatstressonbloodbrainbarrierintegrityinipscellderivedmicrovascularendothelialcellmodels AT kentaroshimizu effectofheatstressonbloodbrainbarrierintegrityinipscellderivedmicrovascularendothelialcellmodels AT yasuhirokokubu effectofheatstressonbloodbrainbarrierintegrityinipscellderivedmicrovascularendothelialcellmodels AT misaenishijima effectofheatstressonbloodbrainbarrierintegrityinipscellderivedmicrovascularendothelialcellmodels AT shukotakeda effectofheatstressonbloodbrainbarrierintegrityinipscellderivedmicrovascularendothelialcellmodels AT hiroshiogura effectofheatstressonbloodbrainbarrierintegrityinipscellderivedmicrovascularendothelialcellmodels AT kenjikawabata effectofheatstressonbloodbrainbarrierintegrityinipscellderivedmicrovascularendothelialcellmodels |