Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation
ABSTRACT Fast-growing bamboo may be a source of high-quality cellulose with the potential to contribute to energy sustainability, if an efficient and low-cost solution to bamboo cellulose decomposition can be developed. This study compared the gut microbiomes of rhinoceros beetle (Trypoxylus dichoto...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2022-10-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02147-22 |
| _version_ | 1797989987560456192 |
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| author | Junhao Huang Linyao Weng Xinqi Zhang Kui Long Xiaojiao An Jinliang Bao Hong Wu Xudong Zhou Shouke Zhang |
| author_facet | Junhao Huang Linyao Weng Xinqi Zhang Kui Long Xiaojiao An Jinliang Bao Hong Wu Xudong Zhou Shouke Zhang |
| author_sort | Junhao Huang |
| collection | DOAJ |
| description | ABSTRACT Fast-growing bamboo may be a source of high-quality cellulose with the potential to contribute to energy sustainability, if an efficient and low-cost solution to bamboo cellulose decomposition can be developed. This study compared the gut microbiomes of rhinoceros beetle (Trypoxylus dichotomus) feeding on bamboo and wood fiber. The results revealed that diet has a distinctive effect on microbial composition in the midgut, including its most abundant microorganisms that in the fermentation and chemoheterotroph pathways. After identifying the 13 efficient bacterial isolates, we constructed a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate to catabolize bamboo lignocellulose. The isolate Enterobacter sp. AZA_4_5 and the natural system showed higher degradation efficiency than other single strains or the artificial system. The results can thus serve as important reference for further research and development of a synthetic bacterial consortium to maximize lignocellulolytic ability. IMPORTANCE Bamboo produces a great yield of lignocellulosic biomass due to its high efficiency in carbon fixing. The gut microbiome of Trypoxylus dichotomus differed between bamboo and wood fiber diets. The lignocellulosic pathways were enriched in the gut bacteria of the bamboo diet. The highly efficient bacterial isolates were identified from midgut, whereas the natural bacterial system as well as one isolate showed the higher degradation efficiency of bamboo lignocellulose. The results indicate that the gut bacteria could provide an effective system to utilize the bamboo lignocellulosic biomass. |
| first_indexed | 2024-04-11T08:29:23Z |
| format | Article |
| id | doaj.art-c2afcda928ba42d9af7d552a59ef090a |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-04-11T08:29:23Z |
| publishDate | 2022-10-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-c2afcda928ba42d9af7d552a59ef090a2022-12-22T04:34:37ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972022-10-0110510.1128/spectrum.02147-22Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose DegradationJunhao Huang0Linyao Weng1Xinqi Zhang2Kui Long3Xiaojiao An4Jinliang Bao5Hong Wu6Xudong Zhou7Shouke Zhang8Department of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, ChinaDepartment of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, ChinaDepartment of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, ChinaDepartment of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, ChinaCollege of Chemistry and Materials Engineering, National Engineering & Technology Research Center for the Comprehensive Utilization of Wood-Based Resources, Zhejiang A&F University, Hangzhou, ChinaShanzhizhou Ecological Agriculture Company Limited, Pan’an, ChinaDepartment of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, ChinaDepartment of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, ChinaDepartment of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, ChinaABSTRACT Fast-growing bamboo may be a source of high-quality cellulose with the potential to contribute to energy sustainability, if an efficient and low-cost solution to bamboo cellulose decomposition can be developed. This study compared the gut microbiomes of rhinoceros beetle (Trypoxylus dichotomus) feeding on bamboo and wood fiber. The results revealed that diet has a distinctive effect on microbial composition in the midgut, including its most abundant microorganisms that in the fermentation and chemoheterotroph pathways. After identifying the 13 efficient bacterial isolates, we constructed a natural bacterial system based on the microbial relative abundance and an artificial bacterial system with equal proportions of each isolate to catabolize bamboo lignocellulose. The isolate Enterobacter sp. AZA_4_5 and the natural system showed higher degradation efficiency than other single strains or the artificial system. The results can thus serve as important reference for further research and development of a synthetic bacterial consortium to maximize lignocellulolytic ability. IMPORTANCE Bamboo produces a great yield of lignocellulosic biomass due to its high efficiency in carbon fixing. The gut microbiome of Trypoxylus dichotomus differed between bamboo and wood fiber diets. The lignocellulosic pathways were enriched in the gut bacteria of the bamboo diet. The highly efficient bacterial isolates were identified from midgut, whereas the natural bacterial system as well as one isolate showed the higher degradation efficiency of bamboo lignocellulose. The results indicate that the gut bacteria could provide an effective system to utilize the bamboo lignocellulosic biomass.https://journals.asm.org/doi/10.1128/spectrum.02147-22bamboo fibercelluloytic activitygut bacteriametabolic pathwayrhinoceros beetle |
| spellingShingle | Junhao Huang Linyao Weng Xinqi Zhang Kui Long Xiaojiao An Jinliang Bao Hong Wu Xudong Zhou Shouke Zhang Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation Microbiology Spectrum bamboo fiber celluloytic activity gut bacteria metabolic pathway rhinoceros beetle |
| title | Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation |
| title_full | Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation |
| title_fullStr | Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation |
| title_full_unstemmed | Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation |
| title_short | Trypoxylus dichotomus Gut Bacteria Provides an Effective System for Bamboo Lignocellulose Degradation |
| title_sort | trypoxylus dichotomus gut bacteria provides an effective system for bamboo lignocellulose degradation |
| topic | bamboo fiber celluloytic activity gut bacteria metabolic pathway rhinoceros beetle |
| url | https://journals.asm.org/doi/10.1128/spectrum.02147-22 |
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