Platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury
The effect of platelet-rich plasma on nerve regeneration remains controversial. In this study, we established a rabbit model of sciatic nerve small-gap defects with preserved epineurium and then filled the gaps with platelet-rich plasma. Twenty-eight rabbits were divided into the following groups (7...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2023-01-01
|
| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2023;volume=18;issue=2;spage=375;epage=381;aulast= |
| _version_ | 1811309638168084480 |
|---|---|
| author | Su-Long Wang Xi-Lin Liu Zhi-Chen Kang Yue-Shu Wang |
| author_facet | Su-Long Wang Xi-Lin Liu Zhi-Chen Kang Yue-Shu Wang |
| author_sort | Su-Long Wang |
| collection | DOAJ |
| description | The effect of platelet-rich plasma on nerve regeneration remains controversial. In this study, we established a rabbit model of sciatic nerve small-gap defects with preserved epineurium and then filled the gaps with platelet-rich plasma. Twenty-eight rabbits were divided into the following groups (7 rabbits/group): model, low-concentration PRP (2.5–3.5-fold concentration of whole blood platelets), medium-concentration PRP (4.5–6.5-fold concentration of whole blood platelets), and high-concentration PRP (7.5–8.5-fold concentration of whole blood platelets). Electrophysiological and histomorphometrical assessments and proteomics analysis were used to evaluate regeneration of the sciatic nerve. Our results showed that platelet-rich plasma containing 4.5–6.5- and 7.5–8.5-fold concentrations of whole blood platelets promoted repair of sciatic nerve injury. Proteomics analysis was performed to investigate the possible mechanism by which platelet-rich plasma promoted nerve regeneration. Proteomics analysis showed that after sciatic nerve injury, platelet-rich plasma increased the expression of integrin subunit β-8 (ITGB8), which participates in angiogenesis, and differentially expressed proteins were mainly enriched in focal adhesion pathways. Additionally, two key proteins, ribosomal protein S27a (RSP27a) and ubiquilin 1 (UBQLN1), which were selected after protein-protein interaction analysis, are involved in the regulation of ubiquitin levels in vivo. These data suggest that platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury by affecting angiogenesis and intracellular ubiquitin levels. |
| first_indexed | 2024-04-13T09:45:04Z |
| format | Article |
| id | doaj.art-c2b573265b7d4fe9976aa49152660dec |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-04-13T09:45:04Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-c2b573265b7d4fe9976aa49152660dec2022-12-22T02:51:47ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742023-01-0118237538110.4103/1673-5374.346461Platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injurySu-Long WangXi-Lin LiuZhi-Chen KangYue-Shu WangThe effect of platelet-rich plasma on nerve regeneration remains controversial. In this study, we established a rabbit model of sciatic nerve small-gap defects with preserved epineurium and then filled the gaps with platelet-rich plasma. Twenty-eight rabbits were divided into the following groups (7 rabbits/group): model, low-concentration PRP (2.5–3.5-fold concentration of whole blood platelets), medium-concentration PRP (4.5–6.5-fold concentration of whole blood platelets), and high-concentration PRP (7.5–8.5-fold concentration of whole blood platelets). Electrophysiological and histomorphometrical assessments and proteomics analysis were used to evaluate regeneration of the sciatic nerve. Our results showed that platelet-rich plasma containing 4.5–6.5- and 7.5–8.5-fold concentrations of whole blood platelets promoted repair of sciatic nerve injury. Proteomics analysis was performed to investigate the possible mechanism by which platelet-rich plasma promoted nerve regeneration. Proteomics analysis showed that after sciatic nerve injury, platelet-rich plasma increased the expression of integrin subunit β-8 (ITGB8), which participates in angiogenesis, and differentially expressed proteins were mainly enriched in focal adhesion pathways. Additionally, two key proteins, ribosomal protein S27a (RSP27a) and ubiquilin 1 (UBQLN1), which were selected after protein-protein interaction analysis, are involved in the regulation of ubiquitin levels in vivo. These data suggest that platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury by affecting angiogenesis and intracellular ubiquitin levels.http://www.nrronline.org/article.asp?issn=1673-5374;year=2023;volume=18;issue=2;spage=375;epage=381;aulast=bioinformatic analysis; itgb8; leukocyte-platelet rich plasma; nerve regeneration; peripheral nerve injury; platelet-rich plasma; proteomic analysis; sciatic nerve injury |
| spellingShingle | Su-Long Wang Xi-Lin Liu Zhi-Chen Kang Yue-Shu Wang Platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury Neural Regeneration Research bioinformatic analysis; itgb8; leukocyte-platelet rich plasma; nerve regeneration; peripheral nerve injury; platelet-rich plasma; proteomic analysis; sciatic nerve injury |
| title | Platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury |
| title_full | Platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury |
| title_fullStr | Platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury |
| title_full_unstemmed | Platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury |
| title_short | Platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury |
| title_sort | platelet rich plasma promotes peripheral nerve regeneration after sciatic nerve injury |
| topic | bioinformatic analysis; itgb8; leukocyte-platelet rich plasma; nerve regeneration; peripheral nerve injury; platelet-rich plasma; proteomic analysis; sciatic nerve injury |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2023;volume=18;issue=2;spage=375;epage=381;aulast= |
| work_keys_str_mv | AT sulongwang plateletrichplasmapromotesperipheralnerveregenerationaftersciaticnerveinjury AT xilinliu plateletrichplasmapromotesperipheralnerveregenerationaftersciaticnerveinjury AT zhichenkang plateletrichplasmapromotesperipheralnerveregenerationaftersciaticnerveinjury AT yueshuwang plateletrichplasmapromotesperipheralnerveregenerationaftersciaticnerveinjury |