Bioactive Phytochemicals from <i>Salix pseudolasiogyne</i> Twigs: Anti-Adipogenic Effect of 2′-<i>O</i>-Acetylsalicortin in 3T3-L1 Cells
<i>Salix pseudolasiogyne</i> (Salicaceae) is a willow tree and has been used as a medicinal herb in Korea to treat pain and fever. As a part of an ongoing study to identify bioactive natural products, potential anti-adipogenic compounds were investigated using the ethanol (EtOH) extract...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/19/12006 |
| Summary: | <i>Salix pseudolasiogyne</i> (Salicaceae) is a willow tree and has been used as a medicinal herb in Korea to treat pain and fever. As a part of an ongoing study to identify bioactive natural products, potential anti-adipogenic compounds were investigated using the ethanol (EtOH) extract of <i>S. pseudolasiogyne</i> twigs. Phytochemical investigation of the EtOH extracts using liquid chromatography–mass spectrometry (LC/MS) led to the separation of two compounds, oregonin (<b>1</b>) and 2′-<i>O</i>-acetylsalicortin (<b>2</b>). The structures of the isolates were identified using nuclear magnetic resonance spectroscopy and LC/MS analysis. To the best of our knowledge, it is the first report identifying oregonin (<b>1</b>) in twigs of <i>S. pseudolasiogyne</i>. Here, we found that the isolated compounds, oregonin (<b>1</b>) and 2′-<i>O</i>-acetylsalicortin (<b>2</b>), showed anti-adipogenic effects during 3T3-L1 cell differentiation. Notably, 2′-<i>O</i>-acetylsalicortin (<b>2</b>), at a concentration of 50 µM, significantly suppressed lipid accumulation. Moreover, the mRNA and protein levels of lipogenic and adipogenic transcription factors were reduced in 2′-<i>O</i>-acetylsalicortin (<b>2</b>)-treated 3T3-L1 cells. Taken together, these results indicate that 2′-<i>O</i>-acetylsalicortin (<b>2</b>), isolated from <i>S. pseudolasiogyne</i> twigs, has the potential to be applied as a therapeutic agent to effectively control adipocyte differentiation, a critical stage in the progression of obesity. |
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| ISSN: | 1661-6596 1422-0067 |