Effect of Diet Consistency on Rat Mandibular Growth: A Geometric Morphometric and Linear Cephalometric Study
Background: Our study intended to investigate the null hypothesis that there is no effect of diet consistency on rat mandibular growth. Methods: A total sample of 24 female wistar rats, 30 days old, was used in this study. In the first group, the rats were fed soft diet and in the second group, they...
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MDPI AG
2022-06-01
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| author | Ioannis A. Tsolakis Christos Verikokos Despoina Perrea Konstantina Alexiou Sotiria Gizani Apostolos I. Tsolakis |
| author_facet | Ioannis A. Tsolakis Christos Verikokos Despoina Perrea Konstantina Alexiou Sotiria Gizani Apostolos I. Tsolakis |
| author_sort | Ioannis A. Tsolakis |
| collection | DOAJ |
| description | Background: Our study intended to investigate the null hypothesis that there is no effect of diet consistency on rat mandibular growth. Methods: A total sample of 24 female wistar rats, 30 days old, was used in this study. In the first group, the rats were fed soft diet and in the second group, they were fed hard diet for 60 days. On the 60th day, the rats were sedated and lateral cephalometric X-rays were taken. Lateral cephalometric X-rays were digitized with 7 craniofacial landmarks for the linear measurements, as well as with 12 curves and 90 landmarks, of which 74 were semilandmarks and 16 were fixed landmarks for morphometric analysis. These landmarks were exposed to Procrustes superimposition and Principal Component Analysis (PCA) to describe the shape variability of the mandible. Results: Means measurements of the soft diet group compared to those of the hard diet group were significantly different in linear and morphometric analysis measurements. The soft diet group of wistar rats revealed significant changes on the condyle (smaller), the angle of the mandible, and on the body of the mandible. Conclusions: Diet consistency affects the craniofacial growth of rats. Soft diet could be responsible for less mandibular growth. |
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| format | Article |
| id | doaj.art-c2be467e33ed4d60b5f21653b74625a1 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T00:23:07Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-c2be467e33ed4d60b5f21653b74625a12023-11-23T15:40:14ZengMDPI AGBiology2079-77372022-06-0111690110.3390/biology11060901Effect of Diet Consistency on Rat Mandibular Growth: A Geometric Morphometric and Linear Cephalometric StudyIoannis A. Tsolakis0Christos Verikokos1Despoina Perrea2Konstantina Alexiou3Sotiria Gizani4Apostolos I. Tsolakis5Department of Orthodontics, School of Dentistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceSecond Department of Surgery, “Laikon Hospital”, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, GreeceLaboratory of Experimental Surgery and Surgical Research “N.S. Christeas”, School of Medicine, National and Kapodistrian University of Athens, 15772 Athens, GreeceDepartment of Oral and Maxillofacial Radiology, School of Dentistry, National and Kapodistrian University of Athens, 10679 Athens, GreeceDepartment of Paediatric Dentistry, Dental School, National and Kapodistrian University of Athens, 11527 Athens, GreeceDepartment of Orthodontics, School of Dentistry, National and Kapodistrian University of Athens, 11527 Athens, GreeceBackground: Our study intended to investigate the null hypothesis that there is no effect of diet consistency on rat mandibular growth. Methods: A total sample of 24 female wistar rats, 30 days old, was used in this study. In the first group, the rats were fed soft diet and in the second group, they were fed hard diet for 60 days. On the 60th day, the rats were sedated and lateral cephalometric X-rays were taken. Lateral cephalometric X-rays were digitized with 7 craniofacial landmarks for the linear measurements, as well as with 12 curves and 90 landmarks, of which 74 were semilandmarks and 16 were fixed landmarks for morphometric analysis. These landmarks were exposed to Procrustes superimposition and Principal Component Analysis (PCA) to describe the shape variability of the mandible. Results: Means measurements of the soft diet group compared to those of the hard diet group were significantly different in linear and morphometric analysis measurements. The soft diet group of wistar rats revealed significant changes on the condyle (smaller), the angle of the mandible, and on the body of the mandible. Conclusions: Diet consistency affects the craniofacial growth of rats. Soft diet could be responsible for less mandibular growth.https://www.mdpi.com/2079-7737/11/6/901growthsoft diethard dietmandible |
| spellingShingle | Ioannis A. Tsolakis Christos Verikokos Despoina Perrea Konstantina Alexiou Sotiria Gizani Apostolos I. Tsolakis Effect of Diet Consistency on Rat Mandibular Growth: A Geometric Morphometric and Linear Cephalometric Study Biology growth soft diet hard diet mandible |
| title | Effect of Diet Consistency on Rat Mandibular Growth: A Geometric Morphometric and Linear Cephalometric Study |
| title_full | Effect of Diet Consistency on Rat Mandibular Growth: A Geometric Morphometric and Linear Cephalometric Study |
| title_fullStr | Effect of Diet Consistency on Rat Mandibular Growth: A Geometric Morphometric and Linear Cephalometric Study |
| title_full_unstemmed | Effect of Diet Consistency on Rat Mandibular Growth: A Geometric Morphometric and Linear Cephalometric Study |
| title_short | Effect of Diet Consistency on Rat Mandibular Growth: A Geometric Morphometric and Linear Cephalometric Study |
| title_sort | effect of diet consistency on rat mandibular growth a geometric morphometric and linear cephalometric study |
| topic | growth soft diet hard diet mandible |
| url | https://www.mdpi.com/2079-7737/11/6/901 |
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