Reduced Cytokine Tumour Necrosis Factor by Pharmacological Intervention in a Preclinical Study
Recent preclinical studies in our laboratory have shown that the bile acid profile is altered during diabetes development and such alteration has been linked to the diabetes-associated inflammatory profile. Hence, this study aimed to investigate if the first-line antidiabetic drug metformin will alt...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/12/7/877 |
| _version_ | 1827619177805905920 |
|---|---|
| author | Armin Mooranian Jacqueline Chester Edan Johnston Corina Mihaela Ionescu Daniel Walker Melissa Jones Susbin Raj Wagle Bozica Kovacevic Thomas Foster Momir Mikov Hani Al-Salami |
| author_facet | Armin Mooranian Jacqueline Chester Edan Johnston Corina Mihaela Ionescu Daniel Walker Melissa Jones Susbin Raj Wagle Bozica Kovacevic Thomas Foster Momir Mikov Hani Al-Salami |
| author_sort | Armin Mooranian |
| collection | DOAJ |
| description | Recent preclinical studies in our laboratory have shown that the bile acid profile is altered during diabetes development and such alteration has been linked to the diabetes-associated inflammatory profile. Hence, this study aimed to investigate if the first-line antidiabetic drug metformin will alter the bile acid profile and diabetes-associated inflammation in a murine model of pre-type 2 diabetes. C57 mice were randomly allocated into three equal groups of eight. Group One was given a low-fat diet (LFD), Group Two was given a high-fat diet (HFD), and Group Three was given an HFD and, upon prediabetes confirmation, daily oral metformin for one month. Blood glucose, glycated haemoglobin, drug concentrations in tissues and faeces, and the inflammatory and bile acid profiles were measured. Metformin showed wide tissue distribution and was also present in faeces. The bile acid profile showed significant alteration due to prediabetes, and although metformin did not completely normalize it, it did exert significant effects on both the bile acid and the inflammatory profiles, suggesting a direct and, to some extent, positive impact, particularly on the diabetes-associated inflammatory profile. |
| first_indexed | 2024-03-09T10:22:35Z |
| format | Article |
| id | doaj.art-c2c2d55124b74b4b8101fd8263641d5c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T10:22:35Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-c2c2d55124b74b4b8101fd8263641d5c2023-12-01T21:55:14ZengMDPI AGBiomolecules2218-273X2022-06-0112787710.3390/biom12070877Reduced Cytokine Tumour Necrosis Factor by Pharmacological Intervention in a Preclinical StudyArmin Mooranian0Jacqueline Chester1Edan Johnston2Corina Mihaela Ionescu3Daniel Walker4Melissa Jones5Susbin Raj Wagle6Bozica Kovacevic7Thomas Foster8Momir Mikov9Hani Al-Salami10The Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, WA 6102, AustraliaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, WA 6102, AustraliaDepartment of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21101 Novi Sad, SerbiaThe Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, WA 6102, AustraliaRecent preclinical studies in our laboratory have shown that the bile acid profile is altered during diabetes development and such alteration has been linked to the diabetes-associated inflammatory profile. Hence, this study aimed to investigate if the first-line antidiabetic drug metformin will alter the bile acid profile and diabetes-associated inflammation in a murine model of pre-type 2 diabetes. C57 mice were randomly allocated into three equal groups of eight. Group One was given a low-fat diet (LFD), Group Two was given a high-fat diet (HFD), and Group Three was given an HFD and, upon prediabetes confirmation, daily oral metformin for one month. Blood glucose, glycated haemoglobin, drug concentrations in tissues and faeces, and the inflammatory and bile acid profiles were measured. Metformin showed wide tissue distribution and was also present in faeces. The bile acid profile showed significant alteration due to prediabetes, and although metformin did not completely normalize it, it did exert significant effects on both the bile acid and the inflammatory profiles, suggesting a direct and, to some extent, positive impact, particularly on the diabetes-associated inflammatory profile.https://www.mdpi.com/2218-273X/12/7/877diabetes mellitusbile acidsinflammationcytokinesinterleukins |
| spellingShingle | Armin Mooranian Jacqueline Chester Edan Johnston Corina Mihaela Ionescu Daniel Walker Melissa Jones Susbin Raj Wagle Bozica Kovacevic Thomas Foster Momir Mikov Hani Al-Salami Reduced Cytokine Tumour Necrosis Factor by Pharmacological Intervention in a Preclinical Study Biomolecules diabetes mellitus bile acids inflammation cytokines interleukins |
| title | Reduced Cytokine Tumour Necrosis Factor by Pharmacological Intervention in a Preclinical Study |
| title_full | Reduced Cytokine Tumour Necrosis Factor by Pharmacological Intervention in a Preclinical Study |
| title_fullStr | Reduced Cytokine Tumour Necrosis Factor by Pharmacological Intervention in a Preclinical Study |
| title_full_unstemmed | Reduced Cytokine Tumour Necrosis Factor by Pharmacological Intervention in a Preclinical Study |
| title_short | Reduced Cytokine Tumour Necrosis Factor by Pharmacological Intervention in a Preclinical Study |
| title_sort | reduced cytokine tumour necrosis factor by pharmacological intervention in a preclinical study |
| topic | diabetes mellitus bile acids inflammation cytokines interleukins |
| url | https://www.mdpi.com/2218-273X/12/7/877 |
| work_keys_str_mv | AT arminmooranian reducedcytokinetumournecrosisfactorbypharmacologicalinterventioninapreclinicalstudy AT jacquelinechester reducedcytokinetumournecrosisfactorbypharmacologicalinterventioninapreclinicalstudy AT edanjohnston reducedcytokinetumournecrosisfactorbypharmacologicalinterventioninapreclinicalstudy AT corinamihaelaionescu reducedcytokinetumournecrosisfactorbypharmacologicalinterventioninapreclinicalstudy AT danielwalker reducedcytokinetumournecrosisfactorbypharmacologicalinterventioninapreclinicalstudy AT melissajones reducedcytokinetumournecrosisfactorbypharmacologicalinterventioninapreclinicalstudy AT susbinrajwagle reducedcytokinetumournecrosisfactorbypharmacologicalinterventioninapreclinicalstudy AT bozicakovacevic reducedcytokinetumournecrosisfactorbypharmacologicalinterventioninapreclinicalstudy AT thomasfoster reducedcytokinetumournecrosisfactorbypharmacologicalinterventioninapreclinicalstudy AT momirmikov reducedcytokinetumournecrosisfactorbypharmacologicalinterventioninapreclinicalstudy AT hanialsalami reducedcytokinetumournecrosisfactorbypharmacologicalinterventioninapreclinicalstudy |