Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors
PurposeModern techniques for improved tumor visualization have the aim to maximize the extent of resection during brain tumor surgery and thus improve patient prognosis. Optical imaging of autofluorescence is a powerful and non-invasive tool to monitor metabolic changes and transformation in brain t...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1105648/full |
| _version_ | 1797902859268784128 |
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| author | David Reichert David Reichert Lisa I. Wadiura Mikael T. Erkkilae Johanna Gesperger Johanna Gesperger Alexandra Lang Thomas Roetzer-Pejrimovsky Jessica Makolli Adelheid Woehrer Marco Wilzbach Christoph Hauger Barbara Kiesel Marco Andreana Angelika Unterhuber Wolfgang Drexler Georg Widhalm Rainer A. Leitgeb Rainer A. Leitgeb |
| author_facet | David Reichert David Reichert Lisa I. Wadiura Mikael T. Erkkilae Johanna Gesperger Johanna Gesperger Alexandra Lang Thomas Roetzer-Pejrimovsky Jessica Makolli Adelheid Woehrer Marco Wilzbach Christoph Hauger Barbara Kiesel Marco Andreana Angelika Unterhuber Wolfgang Drexler Georg Widhalm Rainer A. Leitgeb Rainer A. Leitgeb |
| author_sort | David Reichert |
| collection | DOAJ |
| description | PurposeModern techniques for improved tumor visualization have the aim to maximize the extent of resection during brain tumor surgery and thus improve patient prognosis. Optical imaging of autofluorescence is a powerful and non-invasive tool to monitor metabolic changes and transformation in brain tumors. Cellular redox ratios can be retrieved from fluorescence emitted by the coenzymes reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD). Recent studies point out that the influence of flavin mononucleotide (FMN) has been underestimated.Experimental designFluorescence lifetime imaging and fluorescence spectroscopy were performed through a modified surgical microscope. We acquired 361 flavin fluorescence lifetime (500-580 nm) and fluorescence spectra (430-740 nm) data points on freshly excised different brain tumors: low-grade gliomas (N=17), high-grade gliomas (N=42), meningiomas (N=23), metastases (N=26) and specimens from the non-tumorous brain (N=3).ResultsProtein-bound FMN fluorescence in brain tumors did increase with a shift toward a more glycolytic metabolism (R=-0.87). This increased the average flavin fluorescence lifetime in tumor entities with respect to the non-tumorous brain. Further, these metrics were characteristic for the different tumor entities and showed promise for machine learning based brain tumor classification.ConclusionsOur results shed light on FMN fluorescence in metabolic imaging and outline the potential for supporting the neurosurgeon in visualizing and classifying brain tumor tissue during surgery. |
| first_indexed | 2024-04-10T09:24:44Z |
| format | Article |
| id | doaj.art-c2c599ad28d84f66a89f23773e63cad4 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-10T09:24:44Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-c2c599ad28d84f66a89f23773e63cad42023-02-20T07:24:23ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-02-011310.3389/fonc.2023.11056481105648Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumorsDavid Reichert0David Reichert1Lisa I. Wadiura2Mikael T. Erkkilae3Johanna Gesperger4Johanna Gesperger5Alexandra Lang6Thomas Roetzer-Pejrimovsky7Jessica Makolli8Adelheid Woehrer9Marco Wilzbach10Christoph Hauger11Barbara Kiesel12Marco Andreana13Angelika Unterhuber14Wolfgang Drexler15Georg Widhalm16Rainer A. Leitgeb17Rainer A. Leitgeb18Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, AustriaChristian Doppler Laboratory for Innovative Optical Imaging and its Translation to Medicine (OPTRAMED), Medical University of Vienna, Vienna, AustriaDepartment of Neurosurgery, General Hospital and Medical University of Vienna, Vienna, AustriaCenter for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, AustriaCenter for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, AustriaDivision of Neuropathology and Neurochemistry, Department of Neurology, Medical University of Vienna, Vienna, AustriaDepartment of Neurosurgery, General Hospital and Medical University of Vienna, Vienna, AustriaDivision of Neuropathology and Neurochemistry, Department of Neurology, Medical University of Vienna, Vienna, AustriaDepartment of Neurosurgery, General Hospital and Medical University of Vienna, Vienna, AustriaDivision of Neuropathology and Neurochemistry, Department of Neurology, Medical University of Vienna, Vienna, AustriaAdvanced Development Microsurgery, Carl Zeiss Meditec AG, Oberkochen, GermanyAdvanced Development Microsurgery, Carl Zeiss Meditec AG, Oberkochen, GermanyDepartment of Neurosurgery, General Hospital and Medical University of Vienna, Vienna, AustriaCenter for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, AustriaCenter for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, AustriaCenter for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, AustriaDepartment of Neurosurgery, General Hospital and Medical University of Vienna, Vienna, AustriaCenter for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, AustriaChristian Doppler Laboratory for Innovative Optical Imaging and its Translation to Medicine (OPTRAMED), Medical University of Vienna, Vienna, AustriaPurposeModern techniques for improved tumor visualization have the aim to maximize the extent of resection during brain tumor surgery and thus improve patient prognosis. Optical imaging of autofluorescence is a powerful and non-invasive tool to monitor metabolic changes and transformation in brain tumors. Cellular redox ratios can be retrieved from fluorescence emitted by the coenzymes reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD). Recent studies point out that the influence of flavin mononucleotide (FMN) has been underestimated.Experimental designFluorescence lifetime imaging and fluorescence spectroscopy were performed through a modified surgical microscope. We acquired 361 flavin fluorescence lifetime (500-580 nm) and fluorescence spectra (430-740 nm) data points on freshly excised different brain tumors: low-grade gliomas (N=17), high-grade gliomas (N=42), meningiomas (N=23), metastases (N=26) and specimens from the non-tumorous brain (N=3).ResultsProtein-bound FMN fluorescence in brain tumors did increase with a shift toward a more glycolytic metabolism (R=-0.87). This increased the average flavin fluorescence lifetime in tumor entities with respect to the non-tumorous brain. Further, these metrics were characteristic for the different tumor entities and showed promise for machine learning based brain tumor classification.ConclusionsOur results shed light on FMN fluorescence in metabolic imaging and outline the potential for supporting the neurosurgeon in visualizing and classifying brain tumor tissue during surgery.https://www.frontiersin.org/articles/10.3389/fonc.2023.1105648/fullfluorescence guided surgeryfluorescence lifetime imagingfluorescence spectroscopyoptical redox ratioflavin mononucleotide |
| spellingShingle | David Reichert David Reichert Lisa I. Wadiura Mikael T. Erkkilae Johanna Gesperger Johanna Gesperger Alexandra Lang Thomas Roetzer-Pejrimovsky Jessica Makolli Adelheid Woehrer Marco Wilzbach Christoph Hauger Barbara Kiesel Marco Andreana Angelika Unterhuber Wolfgang Drexler Georg Widhalm Rainer A. Leitgeb Rainer A. Leitgeb Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors Frontiers in Oncology fluorescence guided surgery fluorescence lifetime imaging fluorescence spectroscopy optical redox ratio flavin mononucleotide |
| title | Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors |
| title_full | Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors |
| title_fullStr | Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors |
| title_full_unstemmed | Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors |
| title_short | Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors |
| title_sort | flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors |
| topic | fluorescence guided surgery fluorescence lifetime imaging fluorescence spectroscopy optical redox ratio flavin mononucleotide |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1105648/full |
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